taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
4305878FFFBFFF87FF1CFF0117D3FC14.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/6770801/files/figure.png	http://doi.org/10.5281/zenodo.6770801	FIGURE 2. Polydora tunicola sp. nov. Light micrographs showing the morphology of preserved specimens (holotype: NSMT- Pol H-855). A: entire body. B: anterior end, dorsal view. C: anterior end, lateral view. D: posterior end, lateral view. E: posterior end, dorsal view. F: posterior end, ventral view. Scale bars: (A) = 5 mm; (B–C) = 1 mm; (D–F) = 500 μm.	FIGURE 2. Polydora tunicola sp. nov. Light micrographs showing the morphology of preserved specimens (holotype: NSMT- Pol H-855). A: entire body. B: anterior end, dorsal view. C: anterior end, lateral view. D: posterior end, lateral view. E: posterior end, dorsal view. F: posterior end, ventral view. Scale bars: (A) = 5 mm; (B–C) = 1 mm; (D–F) = 500 μm.	2022-06-28	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka		Zenodo	biologists	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka			
4305878FFFBFFF87FF1CFF0117D3FC14.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/6770803/files/figure.png	http://doi.org/10.5281/zenodo.6770803	FIGURE 3. Polydora tunicola sp. nov. Light micrographs showing the morphology of living (A–D) and fixed (E–I) specimens. A–D, F–I: paratypes (NSMT-Pol P-856), E: non-type larvae. A: anterior end, dorsal view. B: anterior end, lateral view. C: 2–11 chaetigers, lateral view. D: glandular pouches in the 7th to 14th chaetigers. E: larvae from a maternal tube. F: notopodial falcate spines and companion chaetae of the 5th chaetiger. G: neuropodial hooded hooks of 7th chaetiger. H: hooded hooks from neurochaetae of 20th chaetiger. I: hooded hooks from neurochaetae of posterior (60th) chaetiger. br—branchia; ca—caruncle; cc—companion chaetae; gp—glandular pouch; hh—hooded hook; lt—lateral tooth; ma—median antenna; fs—falcate spine; nec—neuropodial capillaries; nel—neuropodial lamella; noc—notopodial capillaries; nol—notopodial lamella; pa—palp; pr— prostomium; sh—shelf; vc—ventral capillaries. Scale bars: (A–D) = 1 mm; (E) = 300 μm; (F–I) = 50 μm.	FIGURE 3. Polydora tunicola sp. nov. Light micrographs showing the morphology of living (A–D) and fixed (E–I) specimens. A–D, F–I: paratypes (NSMT-Pol P-856), E: non-type larvae. A: anterior end, dorsal view. B: anterior end, lateral view. C: 2–11 chaetigers, lateral view. D: glandular pouches in the 7th to 14th chaetigers. E: larvae from a maternal tube. F: notopodial falcate spines and companion chaetae of the 5th chaetiger. G: neuropodial hooded hooks of 7th chaetiger. H: hooded hooks from neurochaetae of 20th chaetiger. I: hooded hooks from neurochaetae of posterior (60th) chaetiger. br—branchia; ca—caruncle; cc—companion chaetae; gp—glandular pouch; hh—hooded hook; lt—lateral tooth; ma—median antenna; fs—falcate spine; nec—neuropodial capillaries; nel—neuropodial lamella; noc—notopodial capillaries; nol—notopodial lamella; pa—palp; pr— prostomium; sh—shelf; vc—ventral capillaries. Scale bars: (A–D) = 1 mm; (E) = 300 μm; (F–I) = 50 μm.	2022-06-28	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka		Zenodo	biologists	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka			
4305878FFFBFFF87FF1CFF0117D3FC14.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/6770805/files/figure.png	http://doi.org/10.5281/zenodo.6770805	FIGURE 4. Polydora tunicola sp. nov. A: palps and mud tubes of Polydora tunicola sp. nov. protruding from their burrows in the tunics of the sessile ascidian Polycarpa cf. cryptocarpa kroboja. B: closeup of palps and mud tubes of Polydora tunicola sp. nov. C, D: tubes of Polydora tunicola sp. nov. concentrated on near siphons of the ascidians. E: juvenile of Polydora tunicola sp. nov. (arrowhead) constructing a mucous tube on the siphons of the ascidians but not yet bore into the tunic. F: cross-section of a host ascidian showing many pairs of boreholes filled with mud tubes (arrowheads) of Polydora tunicola sp. nov. in the host tunic. Photo by O. Hoshino (A–E) and H. Abe (F).	FIGURE 4. Polydora tunicola sp. nov. A: palps and mud tubes of Polydora tunicola sp. nov. protruding from their burrows in the tunics of the sessile ascidian Polycarpa cf. cryptocarpa kroboja. B: closeup of palps and mud tubes of Polydora tunicola sp. nov. C, D: tubes of Polydora tunicola sp. nov. concentrated on near siphons of the ascidians. E: juvenile of Polydora tunicola sp. nov. (arrowhead) constructing a mucous tube on the siphons of the ascidians but not yet bore into the tunic. F: cross-section of a host ascidian showing many pairs of boreholes filled with mud tubes (arrowheads) of Polydora tunicola sp. nov. in the host tunic. Photo by O. Hoshino (A–E) and H. Abe (F).	2022-06-28	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka		Zenodo	biologists	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka			
4305878FFFBFFF87FF1CFF0117D3FC14.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/6770815/files/figure.png	http://doi.org/10.5281/zenodo.6770815	FIGURE 5. Micro-CT images of the host ascidian. A: three-dimensional (3D) reconstructed image of three U-shaped burrows of Polydora tunicola sp. nov. B: two-dimensional reconstructed images of three U-shaped burrows of Polydora tunicola sp. nov. extracted from tomographic data. Arrowheads of figures A and B indicate the apertures of the burrows. C–E: Cross-sectional image of the host. Arrowheads indicate the boreholes of Polydora tunicola sp. nov. in the ascidian tunic. Scale bars = 10 mm.	FIGURE 5. Micro-CT images of the host ascidian. A: three-dimensional (3D) reconstructed image of three U-shaped burrows of Polydora tunicola sp. nov. B: two-dimensional reconstructed images of three U-shaped burrows of Polydora tunicola sp. nov. extracted from tomographic data. Arrowheads of figures A and B indicate the apertures of the burrows. C–E: Cross-sectional image of the host. Arrowheads indicate the boreholes of Polydora tunicola sp. nov. in the ascidian tunic. Scale bars = 10 mm.	2022-06-28	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka		Zenodo	biologists	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka			
4305878FFFBFFF87FF1CFF0117D3FC14.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/6770819/files/figure.png	http://doi.org/10.5281/zenodo.6770819	FIGURE 6. Maximum likelihood tree inferred from concatenated sequences of nuclear 18S and 28S and mitochondrial 16S rRNA gene sequences of Polydora species obtained in this study and from DDBJ/EMBL/GenBank database (Table 1). The gene sequences obtained in this study are highlighted in boldface. SH-aLRT/approximate Bayes support/ultrafast bootstrap support values of ≥80%/≥0.95/≥95%, respectively, are given beside the respective nodes. Nodes with red circles indicate triple high sup- port values of SH-aLRT ≥ 80, approximate Bayes support ≥ 0.95, and ultrafast bootstrap support ≥ 95. The scale bar represents the number of substitutions per site. Sequences of Dipolydora species are used for outgroup rooting. The symbols at the left of the species names indicate the lifestyle based on the available information (Table 1).	FIGURE 6. Maximum likelihood tree inferred from concatenated sequences of nuclear 18S and 28S and mitochondrial 16S rRNA gene sequences of Polydora species obtained in this study and from DDBJ/EMBL/GenBank database (Table 1). The gene sequences obtained in this study are highlighted in boldface. SH-aLRT/approximate Bayes support/ultrafast bootstrap support values of ≥80%/≥0.95/≥95%, respectively, are given beside the respective nodes. Nodes with red circles indicate triple high sup- port values of SH-aLRT ≥ 80, approximate Bayes support ≥ 0.95, and ultrafast bootstrap support ≥ 95. The scale bar represents the number of substitutions per site. Sequences of Dipolydora species are used for outgroup rooting. The symbols at the left of the species names indicate the lifestyle based on the available information (Table 1).	2022-06-28	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka		Zenodo	biologists	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka			
4305878FFFBFFF87FF1CFF0117D3FC14.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/6770823/files/figure.png	http://doi.org/10.5281/zenodo.6770823	FIGURE 7. Results of plate assay (A) and zymographic analysis (B) for cellulase activity of Polydora tunicola sp. nov. Haloes (A: destained areas) and destained bands (B: arrowheads) indicate the cellulose decomposition (exhibition of cellulase activity). PC = positive control (crystalline style of a brackish water clam Corbicula japonica), NC = negative control.	FIGURE 7. Results of plate assay (A) and zymographic analysis (B) for cellulase activity of Polydora tunicola sp. nov. Haloes (A: destained areas) and destained bands (B: arrowheads) indicate the cellulose decomposition (exhibition of cellulase activity). PC = positive control (crystalline style of a brackish water clam Corbicula japonica), NC = negative control.	2022-06-28	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka		Zenodo	biologists	Abe, Hirokazu;Hoshino, Osamu;Yamada, Kazuyuki;Ogino, Tetsuya;Kawaida, Shun;Sato-Okoshi, Waka			
