identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
F224EC3C5E2A56E8A6E1C292EAA59EA2.text	F224EC3C5E2A56E8A6E1C292EAA59EA2.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Spongiosarcinopsis qinghaiensis Q. YAN & G. LIU 2022	<html xmlns:mods="http://www.loc.gov/mods/v3">
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            <p> Spongiosarcinopsis qinghaiensis Q. YAN &amp; G. LIU sp. nov.</p>
            <p>Description.</p>
            <p> Found on the soil surface around brackish lakes. Unicellular, spherical (Figs 1, 2), and cell diameter of 6-15  μm . Mature cells often divide into two cells by means of diads (Fig. 3). Cells contain one net-like chloroplast (Fig. 6), lateral, almost filling the entire cell at maturity (Figs 2, 8), one nucleus, and one pyrenoid covered by starch envelope, although not obvious (Figs 7, 8). </p>
            <p>The vegetative cell wall is rough and there is an unidentified gelatinous layer outside the cell wall (Fig. 9). The pyrenoid is covered with a segmented starch envelope and penetrated by straight thylakoids (Fig. 10).</p>
            <p>Asexual reproduction is achieved through diads or release of aplanospores (Figs 3-5). Aplanosporangia contain eight to 16 spherical aplanospores, and the release process can be observed (Figs 4, 5). No sexual reproduction was observed.</p>
            <p>Etymology.</p>
            <p>The species epithet refers to the Holotype locality (Qinghai Province).</p>
            <p>Type locality.</p>
            <p>Qinghai Lake National Nature Reserve (37°02'N, 100°44'E), Qinghai Province, China; on soil surface.</p>
            <p>Iconotype.</p>
            <p>Fig. 2.</p>
            <p>Holotype.</p>
            <p>QH2015 (HBI), collected by Qiu-Feng Yan and Huan Zhu, 22 September 2020; deposited in the Freshwater Algal Herbarium (HBI), Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China.</p>
            <p>Distribution.</p>
            <p>At present, the algal isolate is only found in China. It grows on loose and moist soil surfaces around brackish lakes.</p>
            <p>Authentic culture.</p>
            <p> Culture strain FACHB-3451 is deposited in the Freshwater  Algae Specimen Station, Institute of Hydrobiology, Chinese Academy of Sciences (http://algae.ihb.ac.cn/). </p>
            <p> Therefore,  Spongiosarcinopsis qinghaiensis was found to be different from  Spongiosarcinopsis terrestris , with respect to young cell shape, habitat, size of vegetative cells, and number of pyrenoids. Registration. http://phycobank.org/103211. </p>
            <p>Phylogenetic analyses</p>
            <p> The 18S rDNA alignment applied 45 sequences (including 15  Protosiphonaceae sequences), which consisted of 1686 sites, out of which 300 (17.8%) and 208 (12.3%) were variable and parsimony-informative sites, respectively. Nineteen ITS sequences were used for alignment and 546 sites, out of which 327 (59.9%) and 232 (42.5%) were variable and parsimony-informative sites, respectively. Table 1 presents detailed information about the alignment and nucleotide substitution in 18S rDNA and ITS concatenated phylogenies for molecular phylogenetic analysis. </p>
            <p> The phylogenetic tree was constructed using the Bayesian approach based on 18S rDNA and ITS alignments (Figs 11, 12), with the Bayesian posterior probabilities and ML bootstrap support reported. The topology of our 18S rDNA phylogeny (Fig. 11) is essentially consistent with that reported in previous studies (Temraleeva et al. 2017). The tree based on 18S rDNA showed a strong  Spongiosarcinopsis clade, comprising the algal strains isolated in the present study; hence, the algal isolates were classified into the genus  Spongiosarcinopsis (Fig. 11). The tree based on ITS sequences prsesented the interspecific relationships within the genus  Spongiosarcinopsis , and the algal strain isolated in the present study formed a distinct branch separate from  Spongiosarcinopsis terrestris (Fig. 12). </p>
            <p>ITS2 secondary structure</p>
            <p> The ITS2 secondary structure was annotated, and two  Spongiosarcinopsis strains were detected (Fig. 13), namely,  Spongiosarcinopsis terrestris (MF687232) and  Spongiosarcinopsis qinghaiensis (OM333733). The differences between the two strains of algae were compared. Three CBCs and four hemi-compensatory base changes (h-CBCs) were detected between the two strains. </p>
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	https://treatment.plazi.org/id/F224EC3C5E2A56E8A6E1C292EAA59EA2	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Yan, Qiufeng;Zhu, Huan;Fang, Jiao;Liu, Benwen;Liu, Guoxiang	Yan, Qiufeng, Zhu, Huan, Fang, Jiao, Liu, Benwen, Liu, Guoxiang (2022): Taxonomic study of a novel terrestrial alga, Spongiosarcinopsis qinghaiensis sp. nov. (Protosiphonaceae, Chlorophyta), from the Qinghai-Tibet Plateau. PhytoKeys 204: 83-95, DOI: http://dx.doi.org/10.3897/phytokeys.204.84886, URL: http://dx.doi.org/10.3897/phytokeys.204.84886
