taxonID	type	description	language	source
714487C0FFCAFFFB02E7FB5DD797FA1B.taxon	discussion	The adult males of this subgroup are characterized by the following features. Antenna with eight flagellomeres and reduced setae of plume. Head with separate, weak frontal protrusions. Eyes hairy; frontal setae few or absent. Wings sometimes reduced, with rounded, often small anal lobe. Hypopygium with three-lobed segment IX, lateral lobes (laterosternites) often extending beyond posterior margin of the middle part (tergite IX). Tergite bands usually U or Y-shaped. Transverse sternapodeme usually triangular, often with a spire-shaped apex. Anal point bent downwards, sometimes strongly reduced. Gonocoxite with a knob-like superior volsella covered with microtrichia, sometimes with short setae. Gonostylus with 3 – 5 apical teeth and megaseta appearing as a wide terminal spine.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCAFFFE02E7F9C1D265FC13.taxon	description	(Figs. 1 – 7)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCAFFFE02E7F9C1D265FC13.taxon	materials_examined	Material examined. CANADA: 1 adult male, Jasper-Banff Area, Rocky Mountains, 13. VI. 1957, leg. L. Brundin; U. S. A.: 3 adult males, Alaska, Hebert River, about 0.5 mi. downstream of glacier, 20. II. 2010, leg. J. Hudson; RUSSIA: 1 adult male, Kamchatka, Avacha River, 11. VI. 1970, leg. V. Levanidov; 1 adult male, the same data, except, Korf Bay, unnamed stream near Tilichiki Village, 8. VIII. 1972, leg. E. Nikolaeva; 5 adult males, the same data, except, Pravaya Kamchatka River, 7 – 8. VII. 1996, leg. E. Makarchenko; 2 adult males, Chukotka Autonomous Okrug, Anadyrskyi District, unnamed stream of Velikaya River basin, 1. VIII. 1980, leg. E. Makarchenko; 3 adult males, Kurile Islands, Kunashir Island, Sernovodsk Village, Tiurino River, 24. IV. 1978, leg. E. Makarchenko; 3 adult males, the same data, except Onekotan Island, Rezvyi Stream, 7. VIII. 1996, leg. V. Teslenko; 1 adult male, the same data, except Shiashkatan Island, 12. VIII. 1996, leg. V. Teslenko; 3 adult males, Magadan Region, Olskyi District, 137 km of Kolymskaya Road, Ola River, 13. V. 2017, 60.412194 N, 151.514564 E, leg. Е. Khamenkova; 2 adult males, the same data, except, mouth of Ola River, 03. V. 2019, 59.580514 N, 151.272686 E, leg. Е. Khamenkova; 3 adult males, Khabarovsk Territory, Solnechnyi District, Gornyi Village, Silinka River, 22. VII. 1985, leg. E. Makarchenko.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCAFFFE02E7F9C1D265FC13.taxon	description	Description Adult male (n = 10, except when otherwise stated). Total length 2.6 – 3.3 mm. Total length / wing length 0.95 – 1.15. Coloration. Dark brown to black. Wing greyish, with brownish veins. Head. Eyes hairy, reniform. Temporal setae including 2 – 3 frontals and 8 – 10 verticals. Clypeus with 2 – 4 setae. Antenna with 8 flagellomeres and reduced plume of setae (Fig. 4); terminal flagellomere with 1 – 2 subapical setae, 16 – 28 μm long. Length of 1 – 8 flagellomeres (μm): 104 – 120, 34 – 50, 32 – 42, 27 – 38, 29 – 34, 24 – 36, 34 – 48, 104 – 140; AR 0.33 – 0.42. Palpomere length (μm): 36 – 40, 56 – 72, 88 – 92, 80 – 92, 100 – 136. Palpomere 3 in distal part with sensilla capitata with diameter 16 – 20 μm. Head width / palpal length 1.27 – 1.39. Antennal length / palpal length 1.0 – 1.37. Thorax. Antepronotum with 8 – 13 ventrolateral setae. Dorsocentrals 7 – 11, prealars 2 – 5, scutellars 24 – 26. Wing. Length 2.60 – 3.60 mm, width 0.76 – 0.98 mm. Costal extension absent. Anal lobe rounded, sometimes slightly reduced. Squama with 9 – 21 setae. R and R 1 with 6 – 20 setae, R 4 + 5 with 6 – 15 setae. RM / MCu 2.4 – 2.8. Legs. Spur of front tibia 26 – 42 µm long. Spurs of mid tibia 38 – 40 µm and 36 – 48 µm long. Spurs of hind tibia 50 – 76 µm and 29 – 42 µm long. Hind tibial comb with 15 – 24 setae. Length (μm) and proportions of leg segments for males from Kurile Island, Amur River basin, Chukotka, Kamchatka and North America are as in Table 1. Most long legs and different proportions of leg segments have specimens from Magadan Region and for them the data are given in Table 2. Hypopygium (Figs. 1 – 3, 5 – 7). Laterosternites not protruding beyond the posterior margin of tergite IX in East Palaearctic specimens (Figs. 2 – 3) or slightly (60 – 68 µm) extending beyond posterior margin of tergite IX in Nearctic adult males (Figs. 6 – 7). Posterior margin of tergite IX partly straight to slightly rounded, with some weak setae along, 9 – 10 µm long. Laterosternites with 9 – 12 weak setae, 9 – 15 µm long. Tergite bands broad and distinctly U-shaped (Figs. 2 – 3, 6 – 7). Anal point often visible in dorsal view, angled downwards (Fig. 1), 25.2 – 50.4 µm long; ratio of anal point length to gonostylus length 0.15 – 0.24. Transverse sternapodeme (TSA) triangular, usually with a spire-shaped apex (Figs. 5 – 6), 72 – 160 µm high, 180 – 224 µm wide at the base; TSA height / TSA width 0.40 – 0.80. Gonocoxite 360 – 400 µm long; superior volsella rounded, with microtrichia and sometimes short setae (Fig. 5). Gonostylus 192 – 208 µm long, weakly curved, gradually narrowing towards distal end, with 3 – 4 apical teeth of approximately the same size, with strong setae between them and megaseta which in form of wide terminal spine (Figs. 2 – 3, 6 – 7), 8 – 12 μm long; HR 1.75 – 2.20. Pupa was described by Tokunaga (1936). Larva unknown.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCAFFFE02E7F9C1D265FC13.taxon	discussion	Remarks. As noted above, the revision revealed that males from the Eastern Palaearctic are characterized by hypopygium with laterosternites that do not extend beyond the posterior margin of tergite IX, while in males from North America (Alaska and Alberta), the laterosternites extend beyond the posterior margin of tergite IX. Therefore, it is necessary to change the diagnosis of the species given by Willassen (1986) in which he indicated that the laterosternites extend beyond the posterior margin of tergite IX. This error is due to the fact that Willassen analyzed the material only from Alaska and ignored the figure of a male hypopygium from Kamchatka (Makarchenko 1980, Fig. 3 A), in which laterosternites do not extend beyond the posterior margin of tergite IX. It is possible that different species inhabit the Eastern Palaearctic and Nearctic, but to confirm this a comparison with the support of DNA barcoding is recommended. Also noteworthy are adult males from the Magadan Region (Ola River basin) possessing the longest legs and leg’s indexes differing from other populations, namely LR 1 0.58, BV 1 4.60 – 4.79, SV 1 3.53 – 3.64 in specimens from Magadan Region and LR 1 0.62 – 0.67, BV 1 3.98 – 4.38, SV 1 3.04 – 3.27 in specimens from other regions.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCAFFFE02E7F9C1D265FC13.taxon	distribution	Distribution. Known from Japan, Kurile Islands, Kamchatka, Magadan Region, U. S. A. (Alaska) and Canada (Alberta).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCFFFFF02E7FBC9D78EF9FC.taxon	description	(Figs. 8 – 11)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCFFFFF02E7FBC9D78EF9FC.taxon	materials_examined	Material examined. RUSSIA: 15 adult males, Magadan Region, Tenkinsky District, neighborhood of Sibit-Tyellakh Village, spurs of the peak Vlastny, Olen’ Stream (Kolyma River basin), alt. 1300 – 1400 m a. s. l., 27 – 30. VII. 1977, leg. Е. Makarchenko; 10 adult males, Chukotka, Wrangel Island, upper stream of Somnitelnaya River, 22. VII. 1978, leg. E. Makarchenko; 33 adult males, the same data, except Krasnyi Flag River, middle stream, 4. VII. 1979, leg. E. Makarchenko and M. Makarchenko; 138 adult males, 19 pupae and 65 larvae, the same data, except unnamed stream in upper part of Somnitelnaya River, 14. VII – 2. VIII. 1979, leg. E. Makarchenko and M. Makarchenko; 1 adult male, Severnaya Zemlya Archipelago, Bolshevik Island, Mikoyan Bay, Chernaya River, 1. IX. 2019, 79.20768 N, 102.31090 E, leg. A. Krasheninnikov.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCFFFFF02E7FBC9D78EF9FC.taxon	description	Description Adult male (n = 16). Total length 2.4 – 4.2 mm. Total length / wing length 0.92 – 1.23. Coloration. Dark brown to black; wing grayish, veins yellowish brown. Head. Eyes hairy, microtrichia visible along lateral eye margin when head is viewed from front. Temporal setae including 3 frontals and 8 verticals. Clypeus with 2 – 5 setae. Antenna with 8 flagellomeres and reduced plume of setae, 24 – 48 μm long; number of setae in flagellomeres 1 – 7, respectively 2 – 3: 2: 1 – 2: 1 – 2: 2: 2: 4; terminal flagellomere with 3 subapical setae, 12 – 16 μm long and with 3 setae basally, 32 – 36 μm long; pedicel with 3 setae. Flagellomeres 1 – 8 length (μm): 38 – 76 – 109, 36 – 55, 28 – 50, 24 – 42, 24 – 38, 24 – 38, 24 – 46, 96 – 134; AR 0.30 – 0.46. Palpomeres lengths (in μm): 32 – 40; 40 – 80; 72 – 109; 60 – 109; 78 – 143. Palpomere 3 in distal part with sensilla capitata with diameter 16 μm. Antennal length / palpal length 1.18 – 1.45. Thorax. Antepronotum with 3 – 15 ventrolateral setae. Dorsocentrals 9 – 12, prealars 4 – 10, scutellars 7 – 22. Wing. Length 2.24 – 3.79 mm, width 0.96 – 1.25 mm. R and R 1 with 6 – 26 setae; R 4 + 5 with 5 – 9 setae in distal part. Costa extension 49 μm long. RM / MCu 2.0 – 4.6. Anal lobe well developed, rounded. Squama with 13 – 27 setae. VR 0.93. Legs. Spur of front tibia 31 – 46 μm long; spurs of mid tibia 20 – 46 μm long; of hind tibia 44 – 80 μm and 30 – 46 μm long. Hind tibial comb with 9 – 21 setae. Lengths and proportions of leg segments as in Table 3. We did not include in this table the data on the male from the Severnaya Zemlya Archipelago which are given in the article by Krasheninnikov et al. (2020). Hypopygium (Figs. 8 – 11). Laterosternites very extend beyond posterior margin of tergite IX, evenly sclerotized and with relatively dense 5 – 9 setae, 16 – 20 μm long. Posterior margin of tergite IX gently rounded, with 4 – 5 setae (from one side), 8 – 12 μm long. Tergite bands weak, widely U-shaped (semi-circular) (Figs. 8 – 9). Anal point visible in dorsal view, relatively well developed and blunt-tipped, angled downwards, without microtrichia (Fig. 10), 52 – 143 μm long; ratio of anal point length to gonostylus length 0.36 – 0.64. Transverse sternapodeme 68 μm high, triangular, with rounded apex (Figs. 8 – 9). Gonocoxite 360 – 400 µm long; superior volsella rounded, fairly well developed, slightly produced disto-medially, with numerous microtrichia. Gonostylus very strongly curved, broadest in basal 0.4 – 0.5, then abruptly narrowing, with 3 apical teeth of approximately the same size, with strong 3 - 4 setae between them and megaseta which in form of wide terminal spine (Fig. 11), 8 – 12 μm long; HR 1.60 - 2.27. Pupa and larva are described by Makarchenko (1981).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCFFFFF02E7FBC9D78EF9FC.taxon	discussion	Remarks. According to their main features, specimens from North America and Eurasia are close together, only slightly different is the male from Severnaya Zemlya, with total length 2.4 mm, wing length 2.24 mm, length of the anal point 52 μm, while males from North America, Wrangel Island and Kolyma River basin are 2.8 – 4.2 mm long, with wing length 2.52 – 3.79, anal point length 84 – 143 μm. We noted earlier that this species is closely related to D. alpina Tokunaga and D. davisi Edwards (Makarchenko 1980) as well as to D. saetheri Willassen and D. serratosioi Willassen (Makarchenko 1985). The barcoding data (see below) confirm a close similarity of D. amplexivirilia with the last two species.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCFFFFF02E7FBC9D78EF9FC.taxon	distribution	Distribution. Holarctic arcto-alpine species. Known from Canada: Alberta, British Columbia, Northwest Territories, U. S. A.: Montana, Washington, Alaska (Hansen & Cook 1976; Namayandeh 2022) and Russia: Severnaya Zemlya Archipelago (Krasheninnikov et al. 2020), upper streams of Kolyma River, Vrangel Island.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCEFFF202E7F9B1D248FE77.taxon	description	(Figs. 12 – 15)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCEFFF202E7F9B1D248FE77.taxon	materials_examined	Material examined. RUSSIA: 5 adult males, Chukotka Peninsula, The Gilmimliveem River in the area of thermal mineral springs of the Ioni Lake basin., 4. VIII. 1973, leg. I. Levanidova; 5 adult males, the same data, except for the surroundings of the Uelen Village, Nevidimka Stream, 12. VIII. 1973, leg. I. Levanidova; 5 adult males, Chukotka, Wrangel Island, Somnitelnaya River, 23. VII. 1978, leg. E. Makarchenko; 5 adult males, Magadan Region, Tenkinsky District, neighborhood of Sibit-Tyellakh Village, spurs of the peak Vlastny, Olen’ Stream (Kolyma River basin), alt. 1300 – 1400 m a. s. l., 12 – 17. VI. 1978, leg. Е. Makarchenko.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCEFFF202E7F9B1D248FE77.taxon	description	Description Adult male (n = 20). Total length 2.60 – 3.25 mm. Total length / wing length 1.04 – 1.49. Coloration. Dark brown to black; wing grayish, veins yellowish brown. Head. Eyes hairy. Temporal setae including 0 – 1 orbitals, 9 – 15 verticals and 2 – 6 postorbitals. Clypeus with 4 – 8 setae. Antenna with 8 flagellomeres and reduced plume of setae 20 – 50 μm long. Flagellomeres 1 – 8 length (μm): 88 – 126, 34 – 50, 29 – 46, 29 – 36, 25 – 38, 25 – 34, 34 – 42, 88 – 126; terminal flagellomere with 1 – 2 subapical setae, 12 – 20 μm long and with 3 – 4 setae basally, 36 – 40 μm long; AR 0.28 – 0.38. Palpomeres lengths (in μm): 32 – 40; 46 – 76; 80 – 109; 71 – 101; 113 – 143. Palpomere 3 in distal part with sensilla capitata with diameter 14 – 16 μm. Antennal length / palpal length 1.12 – 1.46. Thorax. Antepronotum with 8 – 11 ventrolateral setae. Dorsocentrals 7 – 12, prealars 2 – 6, scutellars 11 – 24. Wing. Length 2.34 – 2.91 mm, width 0.72 – 0.93 mm. R and R 1 with 6 – 16 setae; R 4 + 5 with 2 – 7 setae in distal part. RM / MCu 2.0 – 3.2. Anal lobe rounded, often reduced. Squama with 19 – 21 setae. Legs. Spur of front tibia 20 – 38 μm long; spurs of mid tibia 29 – 50 μm and 27 – 50 μm long; of hind tibia 20 – 63 μm and 51 – 126 μm long. Hind tibial comb with 14 – 21 setae. Lengths and proportions of leg segments as in Table 4. Hypopygium (Figs. 12 – 15). Laterosternites well extended beyond posterior margin of tergite IX. Tergite IX with weak setae along gently rounded posterior margin; tergite bands Y-shaped (Figs. 12 – 13); anal point reduced, not visible in dorsal view, angled downwards, without microtrichia (Fig. 14), 11 – 25 μm long; ratio of anal point length to gonostylus length 0.07 – 0.12. Gonocoxite massive, inner side densely covered with microtrichia and short setae, superior volsella with microtrichia and some short setae. Gonostylus weakly curved with 3 – 5 apical teeth and megaseta which in form of wide terminal spine (Fig. 15). HR 1.77 – 2.50. Pupa and larva were described by Saether (1968).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCEFFF202E7F9B1D248FE77.taxon	discussion	Remarks. Before the revision of the Diamesa davisi group by Willassen (1986) we identified this species as D. davisi Edwards. D. saetheri and D. davisi are very close related morphologically and possibly belong to the same species. To clarify the relationship of these two species, it is necessary in the future to obtain molecular genetic data for D. davisi from the type locality. Before this we adhere to Willassen’s position on taxonomy of these species. Males from Wrangel Island and the Kolyma River basin are slightly larger than those from other regions, but the leg indices and other data are similar for all populations (Table 5). The most interesting specimens are from the highlands of the Kolyma River basin. In two out of five males the wings were noticeably reduced. The ratio of total length to wing length is 1.32 – 1.49, anal lobe reduced, and wing veins are indistinct. A similar reduction was noted earlier for D. steinboecki Goetghebuer (Serra-Tosio 1974) and D. serratosioi Willassen (Willassen 1986).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFCEFFF202E7F9B1D248FE77.taxon	distribution	Distribution. Known from Norway and Russian Far East (Chukotka Peninsula, Wrangel Island and Kolyma River basin).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC3FFF302E7FE2DD2DFFE77.taxon	description	(Figs. 16 – 18)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC3FFF302E7FE2DD2DFFE77.taxon	materials_examined	Material examined. U. S. A.: Paratype, adult male, Alaska, Glacier Bay, Wolf Creek, 12. VIII. 1979, leg. A. M. Milner, Z. M. Bergen Type N 83; 1 adult male, Alaska, Juneau, Hebert River, about 0.5 mi. downstream of glacier, 20. II. 2010, 58.532 N, 134.692 E, leg. J. Hudson; 2 adult males, the same data, except 28. X. 2012, leg. J. Hudson; 1 adult male, Michigan, Washtenaw Co., Huron River, Hudson Mills Metro Park, 23. III. 2013, leg. P. Hudson.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC3FFF302E7FE2DD2DFFE77.taxon	description	Description Adult male (n = 4, except when otherwise stated). Coloration. Dark brown to black. Wings greyish, with brownish veins. Head (Fig. 18). Eyes hairy, reniform. Temporal setae including 1 – 2 frontals, 6 verticals and 9 postorbitals. Clypeus with 2 – 4 setae. Antenna with 8 flagellomeres and reduced plume of setae; terminal flagellomere with 3 – 4 basal setae, 60 – 64 μm long and 2 subapical setae, 16 – 20 μm long. Length of 1 – 8 flagellomeres (μm): 84 – 92, 28 – 48, 32 – 38, 28 – 32, 30 – 32, 24 – 32, 40 – 48, 104 – 116; AR 0.35 – 0.39. Palpomere length (μm): 28 – 40, 48 – 60, 88 – 96, 80 – 88, 96 – 128. Palpomere 3 in distal part with sensilla capitata with diameter 16 – 20 μm. Head width / palpal length 1.15 – 1.21. Antennal length / palpal length 1.04 – 1.14. Thorax. Antepronotum with 7 – 10 ventrolateral setae. Dorsocentrals 5 – 9, prealars 2 – 3, scutellars 10 – 19, epimeron II with 0 – 1 seta. Wing. Length 2.65 – 2.76 mm, width 0.90 – 0.92 mm. Costal extension absent. Anal lobe rounded, sometimes slightly reduced. Squama with 13 – 19 setae. R and R 1 with 10 – 13 setae, R 4 + 5 with 4 – 6. R 2 + 3 visible only in basal part. RM / MCu 2.4 – 2.6. Legs. Spur of front tibia 32 – 36 µm long. Spurs of mid tibia 36 – 40 µm long. Spurs of hind tibia 60 – 72 µm and 36 µm long. Hind tibial comb with 17 – 21 setae. Length (μm) and proportions of leg segments as in Table 6. Hypopygium (Figs. 16 – 17). Laterosternites very extended beyond posterior margin of tergite IX, divided into two parts by curved apodeme, ventro-caudal part more weakly sclerotized than the rest of hypopygium, with weak setae and microtrichia. Tergite IX with posterior margin from gently rounded to partly straight, tergite bands Yshaped, anal point reduced. Transverse sternapodeme (TSA) triangular, usually with a spire-shaped apex (Figs. 16 – 17), 100 – 112 µm high, 180 – 188 µm wide at the base; TSA height / TSA width 0.56 – 0.60. Gonocoxite 280 – 292 µm long; superior volsella with dense microtrichia, without setae. Gonostylus 180 – 200 µm long, curved, narrowed about 0.5 from base, with 3 – 5 apical teeth and megaseta which in form of wide terminal spine, 8 – 10 μm long; HR 1.46 – 1.62. Pupa and larva unknown.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC3FFF302E7FE2DD2DFFE77.taxon	distribution	Distribution. Rare Nearctic species, known only from U. S. A. (Alaska ans Michigan) and Canada (Alberta) (Ashe & O’Connor 2009).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC2FFF302E7FE2DD626FD18.taxon	discussion	Remarks. We do not have additional material to study morphology and to perform DNA barcoding of this species, so we accept Willassen (1986) conclusions.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC2FFF302E7FE2DD626FD18.taxon	distribution	Distribution. Nearctic species, known from Canada, Greenland and U. S. A. (Ashe & O’Connor 2009).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC2FFF102E7FCCED757FBA6.taxon	description	(Figs. 19 – 22)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC2FFF102E7FCCED757FBA6.taxon	materials_examined	Material examined. NORWAY: Paratype adult male, Z. M. Bergen Type N 41, slide NE 61; Ekse, Hoi, Vaksdal, 24 – 30. VI. 1976, leg. T. Andersen. RUSSIA: 3 adult males, Republic of Khakassia, Tashtypsky District, unnamed stream in the basin of the Bolshoy On River, ultraviolet lamp, 51 ° 42 ’ 58.2 ” N 89 ° 51 ’ 25.8 ” E, altitude 1953 m a. s. l., 8 – 9. VIII. 2020, leg. V. Dragan; 2 adult males, Chukotka Autonomous Okrug, Anadyrskyi District, unnamed stream of Velikaya River basin, 1. VIII. 1980, leg. E. Makarchenko; 2 adult males, the same data except Chukotsky District, Chegitun River, 23. VII. 1981, leg. E. Makarchenko; 2 adult males, Khabarovsk Territory, Solnechnyi District, Gornyi Village, Silinka River (Amur River basin), 26. VII. 1983, leg. E. Makarchenko; 6 adult males, Kamchatka, unnamed stream, about 30 km from Esso Village, light trap, 9. VII. 1996, leg. E. Makarchenko; 2 adult males, Magadan Region, Olskyi District, 137 km of Kolymskaya Road, Ola River, 1. V. 2016, 60.412194 N, 151.514564 E, leg. Е. Khamenkova.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC2FFF102E7FCCED757FBA6.taxon	description	Description Adult male (n = 6, except when otherwise stated). Total length 2.3 – 3.0 mm. Total length / wing length 0.82 – 1.0. Coloration. Dark brown to brown. Legs brown, sometimes basal 1 / 3 of femur brownish-yellow. Wings grayish to gray, venation brownish. Head. Eyes hairy, reniform. Temporal setae including 3 – 4 frontals, 5 – 6 verticals. Clypeus with 2 – 7 setae. Antenna with 8 flagellomeres and reduced plume of setae; terminal flagellomere with 2 subapical setae, 24 – 32 μm long. Length of 1 – 8 flagellomeres (μm): 68 – 104, 36 – 48, 36 – 40, 28 – 32, 28 – 32, 24 – 28, 28 – 36, 68 – 116; AR 0.25 – 0.42. Palpomere length (μm): 28 – 48, 40 – 48, 76 – 116, 64 – 100, 108 – 156. Palpomere 3 in distal part with sensilla capitata with diameter 12 – 16 μm. Head width / palpal length 1.05 – 1.22. Antennal length / palpal length 0.90 – 1.08. Thorax. Antepronotum with 6 – 12 ventrolateral setae. Dorsocentrals 7 – 12, prealars 3 – 5, scutellars 22 – 35. Wing. Length 2.44 – 3.0 mm, width 0.72 – 1.1 mm. Anal lobe rounded angular. Squama with 15 – 25 setae. R and R 1 with 17 – 22 setae, R 4 + 5 with 4 – 7 setae. RM / MCu 2.3 – 2.5. Legs. Spur of front tibia 24 – 36 µm long. Spurs of mid tibia 36 – 44 µm and 32 – 48 µm long. Spurs of hind tibia 64 – 84 µm and 32 – 44 µm long. Hind tibial comb with 16 – 18 setae. Length (μm) and proportions of leg segments as in Table 7. Hypopygium (Figs. 19 – 22). Laterosternite IX extending beyond posterior margin of tergite IX by 56 – 80 µm, with weak 18 – 20 setae, 40 – 52 µm long (Figs. 20, 22). Tergite IX with 16 – 18 setae, 20 – 32 µm long and with pale hyaline anal point, 26 – 28 µm long, which is not visible from above, in lateral view pointing down (Fig. 21); posterior margin of tergite IX rounded; tergite bands Y-type (Figs. 20, 22). Transverse sternapodeme (TSA) triangular, peaked, 88 – 140 µm high, 160 – 196 µm wide at the base; TSA height / TSA width 0.52 – 0.83. Gonocoxite 312 – 400 µm long, superior volsella covered with microtrichia and sometimes short setae (Fig. 19). Gonostylus 172 – 204 µm long, broad at base, more or less strongly curved, with megaseta which in form of wide terminal spine, 4 – 5 µm long, 3 – 5 apical teeth and 2 subterminal setae. HR 1.76 – 2.15. Pupa and larva unknown.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC2FFF102E7FCCED757FBA6.taxon	discussion	Remarks. D. serratosioi from Norway and Sweden was previously described as D. davisi Edwards by SerraTosio (1971). We recoded this species from Russia for Khakassia Republic (Makarchenko et al. 2022). This is the first information about the location of D. serratosioi in the Russian Far East.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC2FFF102E7FCCED757FBA6.taxon	distribution	Distribution. Known from Finland, Norway, Sweden (Ashe & O’Connor 2009), Khakassia and Russian Far East (Chukotka and Magadan Region, Amur River basin of Khabarovsk Territory).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC0FFF602E7F8DED031FF07.taxon	discussion	Adult males of this subgroup have large and heavily chitinized, often bent over the dorsal side hypopygium; tergite IX with delicate pubescence of microtrichia directed anteriorly, with long and wedge-shaped or short anal point, angled downwards and covered in basal part with numerous microtrichia forward and laterally directed. Gonocoxite long, broad, with numerous short, anteriorly-directed setae and with slight broad ridge antero-dorsally; inferior volsella with rare exceptions absent; superior volsella in form of angular tubercle or collar. Gonostylus slightly curved, with numerous, proximally directed strong setae, in apical part with 1 megaseta and sometimes 1 tooth. Transverse sternapodeme triangular, with a spire-shaped apex.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC0FFF102E7FB5CD622FA69.taxon	discussion	Remarks. The species has been described and known so far only by one male and two females from North America (California), is close related to D. alpina and requires additional revision, including the use of barcoding.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC0FFF102E7FB5CD622FA69.taxon	distribution	Distribution. Nearctic species, known only from Sonora Pass in California (Ashe & O’Connor 2009).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC0FFF102E7FA1FD70EF928.taxon	discussion	Remarks. The species has been described and known so far only by one male from Japan, is close related to D. alpina and requires additional revision, possibly supported by DNA barcoding.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC0FFF102E7FA1FD70EF928.taxon	distribution	Distribution. Known only from Honshu Island of Japan (Ashe & O’Connor 2009).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC7FFF702E7FEBDD627FF07.taxon	description	(Figs. 23 – 27)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC7FFF702E7FEBDD627FF07.taxon	materials_examined	Material examined. JAPAN: 2 adult males, Honshu, Tochigi Prefecture, Okunikko, Nikko City, Nikko National Park, Toyamazawa River, alt. 1450 m a. s. l., 21. III. 1991, leg. R. Ueno. RUSSIA: 23 adult males, Kurile Islands, Kunashir Island, Sernovodsk Village, Tiurino River, 20. IV. 1978, leg. E. Makarchenko.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC7FFF702E7FEBDD627FF07.taxon	description	Description Adult male (n = 14). Total length 3.1 – 4.2 mm. Wing length 2.76 – 3.17 mm. Total length / wing length 0.90 – 1.40. Coloration. Head, thorax, legs and hypopygium dark brown; antenna and palpomeres brown; abdomen light brown to dark brown; wing greyish, veins brownish. Head. Eyes hairy. Temporal setae including 4 – 12 frontals, 6 – 15 verticals and 4 – 8 postorbitals. Clypeus with 2 – 8 setae. Antenna with 8 flagellomeres and reduced plume of setae (Fig. 23); number and length of these setae on 1 – 7 flagellomeres respectively: 2 – 3 (28 – 40 μm), 1 – 2 (28 – 36 μm), 1 – 2 (26 – 40 μm), 0, 0, 0, 0; terminal flagellomere in basal part with 5 – 6 setae, 60 – 80 μm long and with 2 subapical setae, 40 – 52 μm long. Flagellomeres 1 – 8 length (μm): 71 – 88, 34 – 38, 25 – 34, 25 – 30, 25 – 34, 25 – 34, 32 – 38, 193 – 218; AR 0.68 – 0.81. Antennal length / palp length 1.05 – 1.38. Palpomeres lengths (in μm): 32 – 40; 52 – 63; 88 – 105; 76 – 105; 105 – 147. Palpomere 3 in distal part with sensilla capitata with diameter 20 – 24 μm. Palpomeres 1 – 5 length / head width 0.80 – 1.38. Thorax. Antepronotum with 7 – 12 ventrolateral setae. Dorsocentrals 6 – 10, prealars 5 – 8, scutellars 11 – 24. Wing. R and R 1 with 15 – 25 setae; R 4 + 5 with 8 – 12 setae. Costa extension ca 48 μm long. RM length / MCu length 2.0 – 3.6. Anal lobe well developed, rounded. Squama with 18 – 25 setae 28 – 52 μm long. VR 0.96 – 1.0. Legs. Spur of front tibia 34 – 46 μm long; spurs of mid tibia 40 – 55 μm long; of hind tibia 50 – 71 μm and 32 – 50 μm long. Hind tibial comb with 20 – 22 setae. Lengths and proportions of leg segments as in Table 8. Hypopygium (Figs. 24 – 27). Tergite IX with 7 – 11 setae (from one side), 20 – 40 μm long and wedge-shaped anal point 63 – 136 μm long, angled downwards (Fig. 25) and covered in basal 2 / 3 with numerous microtrichia forward and laterally directed (Figs. 24 – 25). Laterosternite IX with 9 – 11 setae, 28 – 32 μm long. Transverse sternapodeme (TSA) triangular, with a spire-shaped apex (Fig. 27), 124 – 128 µm high, 190 – 196 µm wide at the base; TSA height / TSA width 0.65 – 0.70. Gonocoxite 350 – 492 μm long; inferior volsella absent; superior volsella in form of angular tubercle. Gonostylus slightly curved, 220 – 236 μm long, with numerous, proximally directed strong setae (Figs. 26 – 27), 44 – 52 μm long; megaseta 6 – 8 μm long. HR 1.92 – 2.23. Pupa was described by Tokunaga (1936). Larva unknown.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC7FFF702E7FEBDD627FF07.taxon	distribution	Distribution. East Palaearctic island species. Known from Japan and Kunashir Island (Kurile Islands).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC6FFF402E7FEB6D001FEBF.taxon	description	(Fig. 28)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC6FFF402E7FEB6D001FEBF.taxon	materials_examined	Material examined. U. S. A.: 1 adult male, Alaska, Juneau City, Mendenhall Lake, N 58.4217 E 134.5388, 26. III. 2013, leg. K. Frangos and P. Hudson; 1 adult male, the same data except Nugget Falls, N 58.4270 E 134.5373, 17. VII. 2013, leg. P. Hudson.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC6FFF402E7FEB6D001FEBF.taxon	description	Description Adult male (n = 2). Total length 4.7 mm (n = 1). Wing length 3.44 mm (n = 1). Total length / wing length 1.37. Coloration. Head, thorax, legs and hypopygium dark brown; antenna brown; palpomeres light brown; abdomen light brown to brown; wing greyish, veins yellowish brown. Head. Eyes hairy. Frontal tubercles 16 – 44 μm long, covered with microtrichia. Temporal setae including about 8 – 12 frontals, 13 – 15 verticals, 5 postorbitals. Clypeus with 5 – 6 setae. Antenna with 8 flagellomeres and reduced plume of setae; flagellomeres 1 – 7 with 2 – 3 setae, 24 – 40 μm long; terminal flagellomere in basal part with 4 – 5 setae, 60 – 72 μm long and with 2 subapical setae, 16 – 20 μm long. Flagellomeres 1 – 8 length (μm): 88 – 104, 36 – 40, 40, 32, 32, 26 – 32, 32 – 44, 164 – 248; AR 0.41 – 0.78. Antennal length / palp length 1.09 – 1.21. Palpomeres lengths (in μm): 32 – 40; 56 – 64; 108; 84 – 100; 136 – 156. Palpomere 3 in distal part with sensilla capitata with diameter 20 μm. Palpomeres 1 – 5 length / head width 0.92. Thorax. Antepronotum with 9 – 11 ventrolateral setae. Dorsocentrals 5 – 7, prealars 7, scutellars 9 – 11. Wing. R and R 1 with 25 – 34 setae; R 4 + 5 with 18 – 19 setae. Costa extension 52 – 60 μm long. RM / MCu 2.8 – 3.5. Anal lobe slightly reduced, angularly. Squama with 14 – 17 setae, 52 – 68 μm long. VR 0.96 – 1.0. Legs. Spur of front tibia 40 – 44 μm long; spurs of mid tibia 44 μm long; of hind tibia 74 – 80 μm and 44 – 48 μm long. Hind tibial comb with 15 – 16 setae. Lengths and proportions of leg segments as in Table 9. Hypopygium (Fig. 28). Tergite IX with 11 – 13 setae (from one side), 16 – 24 μm long and wedge-shaped anal point 100 – 140 μm long, covered in basal half with numerous microtrichia forward and laterally directed. Laterosternite IX with 10 – 11 short setae. Transverse sternapodeme triangular, 128 μm long. Gonocoxite 400 – 496 μm long. Inferior volsella finger-like, 40 – 48 μm long and 24 μm width, covered with short setae and microtrichia. Superior volsella in form of collar, covered with microtrichia and short setae. Gonostylus slightly curved, 212 – 246 μm long, with numerous, proximally directed strong setae, 48 – 64 μm long; megaseta 6 – 8 μm long. HR 1.65 – 1.93. Pupa and larva unknown.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC6FFF402E7FEB6D001FEBF.taxon	discussion	Remarks. Male of D. leoniella is very closely related to D. japonica and many years ago senior author misidentified this species as D. japonica (Makarchenko 1981). After obtaining comparative material from North America, it turned out that these two species can be well distinguished by the structure of the hypopygium and some other features (Makarchenko 2021). So, the male of D. leoniella has a finger-shaped inferior volsella and a superior volsella in the form of a collar, while D. japonica without inferior volsella and superior volsella in form of angular tubercle.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC6FFF402E7FEB6D001FEBF.taxon	distribution	Distribution. Nearctic species. Known from U. S. A. — California, Montana, Utah, Washington, Wyoming (Hansen & Cook 1976) and Alaska (recorded for the first time).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC4FFEA02E7FF61D786F8B3.taxon	description	(Figs. 29 – 46)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC4FFEA02E7FF61D786F8B3.taxon	materials_examined	Material examined. RUSSIA: 5 adult males, 3 females, Primorye Territory, Khasansk District, Kedrovaya Pad Nature Reserve, Kedrovaya River, 12. II. 1977, leg. E. Nikolayeva; 1 adult male, the same data except 10. II. 1979, leg. Yu. Shibnev; 1 mature pupa, the same data except 8. II. 1980, leg. E. Makarchenko; 2 adult males, the same data except 19. III. 2016, leg. E. Makarchenko; 2 adult females, the same data except 23. II. 2019, leg. E. Makarchenko; 3 adult males, the same data except Ussuryiskyi District, Ussuryisky Nature Reserve, 1. XII. 1972, leg. I. Chereshnev, L. Budnikova; 1 adult male, the same data except Chuguevskyi District, Elovyi Stream, 31. V. 1977, leg. T. Vshivkova; 1 adult male, the same data except Partizanskyi District, Tigrovaya River, 10. V. 2020, leg. E. Gorovaya; 4 adult males, the same data except Khabarovsk Territory, Nanaisky District, Anyuisky National Park, Pihtsa River (tributary of Gassi Lake), Amur River basin, N 48.47.804, E 136.47.027, 22 – 24. V. 2019, leg. N. Yavorskaya; 1 adult male, the same data except Solnechnyi District, Gornyi Village, Silinka River (Amur River basin), 26. VII. 1983, leg. E. Makarchenko; 1 adult male, 1 female, the same data except Magadan Region, Tenkinskyi District, not far from Sibit-Tyellakh Village, Ozernyi Stream (Kolyma River basin), 3. VI. 1978, S. Kocharina; 2 adult males, the same data except Khasynskyi District, Khasyn River, (Arman River basin) not far from Khasyn Village, 30. VI. 2017, leg. I. Zasypkina; 2 adult females, the same data except Olskyi District, Ola River (upper stream), 1. V. 2016, leg. E. Khamenkova; 1 adult male, 1 female, the same data except 3. V. 2019, leg. E. Khamenkova; 3 adult males, the same data except Kamchatka Territory, Avacha River, 14. VI. 1970, leg. V. Levanidov; 4 adult males, the same data except Sakhalin Island, Forest Park of Yuzhno-Sakhalinsk City, Rogatka River, 15. V. 1984, leg. E. Makarchenko; 2 adult males, the same data except Krasnoselskaya River near Novoalekseevka Village, 2. VI. 1984, leg. E. Makarchenko; 10 adult males, the same data except Chukotka Peninsula, Yoni Lake basin, Gilmimliveem River, 4. VIII. 1973, leg. I. Chereshnev; 2 adult males, the same data except Chegitun River, middle stream, 4. VIII. 1981, leg. Makarchenko. KAZAKHSTAN: 2 adult males, East Kazakhstan region, Katon-Karagai District, Sarymsaqty Mountains (Kazakh Mountain Altai), Arasan River, about 0.7 km below of Bolshoe Rakhmanovskoye Lake, altitude 1790 m above sea level, 5. VII. 2018, 49.535983 N, 86.500633 E, leg. D. Palatov.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC4FFEA02E7FF61D786F8B3.taxon	discussion	Comments. The species D. leona, D. pieta and D. caena were described from North America by Roback (1957) from adult males collected in the same area but at different times. The first two species differed from each other mainly in color and D. caena in reduced wings and the “ absence ” of the anal point. Hansen (Hansen & Cook 1976) comparing the type material of these species, came to the conclusion that D. pieta and D. caena should be junior synonyms of D. leona since all specimens have an anal point and wing reduction is not a diagnostic feature (Hansen & Cook 1976). D. breviala Tokunaga from Japan (Tokunaga 1964) and D. renegata Makarchenko from the Russian Far East (Makarchenko 1977) with reduced wings also were described as adults males. As a result of studying additional material from various regions of the Far East, we came to the conclusion that these both species are identical to the North American D. leona, since have similar hypopygium and occur in populations with both brachypterous and macropterous specimens (Makarchenko 1981). We subscribe to the opinion of SerraTosio (1974) and Hansen & Cook (1976) that the brachypterism of the genus Diamesa is not a basis for taxonomic identification. In the meanwhile it was suggested that D. starmachi Kownacki et Kownacka from the Polish Tatras (Kownacki & Kownacka 1970; Giłka et al 2013) may be also a junior synonym of D. leona, since it has a similar structures of the hypopygium. But it became possible to confirm this only now, after obtaining the molecular genetic data of these two species. DNA-based methods significantly complement traditional taxonomic approaches in that they facilitate to differentiate closely related species or reveal the presence of distinct taxa that are morphologically indistinguishable. The results of DNA barcoding made it possible to confirm conspecificity of D. starmachi from Norway (HQ 941609) and D. leona from Kazakhstan and Russia (ON 834735 – ON 834742). Phylogenetic analysis has shown that the identity of D. starmachi can no more be supported and it is here stated that D. starmachi is a junior synonym of D. leona. Since it’s hard to make a general description for the brachypterous and macropterous forms of males from different regions of the Holarctic and to combine all the data available in the literature, we decided to present the main features of these forms in the Table 10 and give a description of hypopygium only.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC4FFEA02E7FF61D786F8B3.taxon	description	Description Adult male. Hypopygium (Figs. 29 – 46). Large, usually darker than body segments, heavily chitinized and curved dorsally. Tergite IX with delicate pubescence of microtrichia directed anteriorly; anal point in brachypterous forms 42 – 80 μm long and 55 – 108 μm long in macropterous forms, directed downwards at an angle (Fig. 35), almost invisible from above; on straightened tergite IX, viewed from above, anal point often with cut top (Figs. 32 – 34, 42 – 42, 45) (more often in brachypterous forms) or rounded top (Figs. 34, 46), sometimes with sharp top and completely covered with microtrichia (Fig. 40), while in most cases the subapical part of the anal point without microtrichia; gonocoxite long, broad, with numerous short, anteriorly-directed setae (Figs. 29, 41 – 44, 46); gonostylus strong, slightly curved, with numerous short, proximally directed setae; medial surface of gonostylus with fine “ pile ” of microtrichia, with large terminal spine at end, subterminal setae absent (Figs. 30, 34, 36 – 39, 41 – 44, 46). Transverse sternapodeme very strong, triangular, produced to a point antero-medially (Figs. 39, 42 – 44). Anal point length / gonostylus length 0.13 – 0.37). HR 1.50 – 2.47. In the process of studying the material from Sakhalin Island several abnormal males were found that lacked the anal point and the posterior margin of tergite IX was concave in the middle (Fig. 31). Pupa (as D. starmachi) was described by Kownacki & Kownacka (1970) and Makarchenko (1981). Larva was described by Rossaro & Lencioni (2015).	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC4FFEA02E7FF61D786F8B3.taxon	biology_ecology	Biology. The biology of D. leona is very interesting because adult males and females can lead an active life under extreme conditions, namely low air temperatures in winter time. Quite a lot has been published about this for specimens living in North America (Hansen & Cook 1976, Herrmann et al. 1987) and the Polish Tatras (Giłka et al. 2013). But there is very little information about this from the Russian Far East. Therefore, below we present some data on the biology of D. leona from this region of the Palaearctic. In the south of the Russian Far East the species is apparently bivoltine, in the Northeast it is univoltine. Males and females of the first generation in Primorye Territory are brachypterous; they hatch from pupae from early December to early March at a water temperature in the river from 0.1 to 1.5 ºC., and air temperature from – 21 ºC to + 5 ºC. The maximum emergence of adults occurs in mid February. Once three brachypterous males were collected in the Ussuriysky Nature Reserve in the snow on December 1, 1972 at an air temperature of — 21 ºC. Adult insects of the first generation are more like spiders than chironomids. Due to the strong development of the legs, they move well on the ice and are often found on the snow. Males and females of the second generation are macropterous; their emergence occurs in the same area from the end of May to the first half of June. We also encountered brachypterous specimens in the mountains of the Upper Kolyma River basin in early June and in South Sakhalin in the first half of May. Macropterous specimens in South Sakhalin were collected from early May to early June at a water temperature in the rivers from 4 to 17 ºC. On the Chukotka Peninsula the emergence of adults was observed from the second half of July to mid August, in Kamchatka — in mid June. In the Chukotka Peninsula and Kamchatka males and females of D. leona were collected only as macropterous. But despite well developed wings they do not fly and like brachypterous form sit mainly under damp stones along the banks of watercourses. This is where mating takes place. The inability to fly in macropterous specimens is apparently associated with the underdevelopment of the flight muscles. Larvae and pupae live in foothill and mountain streams on stones with a fast current. On Sakhalin Island the largest number of pupae and larvae was observed among algae Hydrurus foetidus Kirchn. D. leona reaches its highest altitude (about 800 m) in the Upper Kolyma River basin.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFC4FFEA02E7FF61D786F8B3.taxon	distribution	Distribution. Widespread arcto-alpine Holarctic species. Known in North America from Canada (New Brunswick and Quebec) and U. S. A. (Colorado, Idaho, Vichigan, Minnesota, Montana, Nevada, New Mexico, Utah, Washington and Wisconsin). In the Palaearctic recorded from China, Japan, Mongolia, Poland, Austria, Germany, Italy, Luxemburg, Slovakia, East Siberia, Russian Far East (Ashe & O’Connor 2009) and Norway.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFDEFFEF02E7FE09D04DFAD2.taxon	description	(Figs. 47 – 60)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFDEFFEF02E7FE09D04DFAD2.taxon	materials_examined	Material examined. NEPAL: 1 adult male, Langtang Region, brook near Khyimjung Glacier, alt. 4150 m a. s. l., 4. VIII. 1999, leg. R. Endo. CHINA: 4 adult males, Xinjiang Uygur Autonomous Region, Tien Shan Mountains, Dabancheng District, Urumqi City District, Sangecha River, 43 ° 40.583 ’ N, 88 ° 17.006 ’ E, alt. 2356 m a. s. l., 13. VII. 2017, leg. D. Palatov. RUSSIA: 8 adult males, Irkutsk Region, Baikal State Biosphere Reserve, KhamarDaban Ridge, Osinovka char, 1400 – 1600 m a. s. l., 20. VII. 2009, leg. Yu. Sundukov; 1 adult male, Republic of Khakassia, Tashtypsky District, unnamed stream in the basin of the Bolshoy On River, ultraviolet lamp, 51.716167 N, 89.857167 E, alt. 1953 m a. s. l., 08 – 09. VIII. 2020, leg. S. Dragan. KAZAKHSTAN: 2 adult males, East Kazakhstan region, Katon-Karagai District, Sarymsaqty Mountains (Kazakh Mountain Altai), Eastern border of Katon-Karagai village, Sarymsaqty River Channel, altitude 1113 m a. s. l., 01. VII. 2018, 49.166283 N, 85.633583 E, leg. D. Palatov. TAJIKISTAN: 4 adult males, spurs of the Gissar Range, Khorangon Gorge, Horangon River, 11 km from the Dushanbe City, 29. II. 1987, leg. L. Zhiltsova; 5 adult males, the same data except Gissar Range, Ramit Nature Reserve, Sardan-Miyona River, alt. 1200 - 1300 m a. s. l., 4 – 5. III. 1987, leg. L. Zhiltsova; 5 adult males, the same data except Varzob River, alt. about 1100 m a. s. l., 11 – 14. III. 1987, leg. L. Zhiltsova; 2 adult males, the same data except Takob River, alt. about 1200 m a. s. l., 14. III. 1987, leg. L. Zhiltsova.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFDEFFEF02E7FE09D04DFAD2.taxon	discussion	Comments. Analysis of our imaginal material showed that the D. khumbugelida male from the type locality in Nepal differs from males of other populations. Therefore, we decided to provide separate descriptions for these males. It is possible that in the future, after obtaining barcoding data for this species from Nepal, it will turn out that these are different species. See more detail in the Remarks.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFDEFFEF02E7FF61D7E1FE50.taxon	discussion	Adult males of this subgroup have antenna with 13 flagellomeres and completely or partly reduced setae of plume. Hypopygium with long anal point, slightly tapering to subapical, with strong anteriorly directed microtrichia in basal half and on tergite IX. Inferior volsella in the form of angular naked projection on the dorsal side of the gonocoxite or like small lobe covered with micro- and macrotrichia and adjacent to a protuberance with some long setae. Superior volsella slightly reduced, in form of rounded angular tubercle or collar. Gonostylus slightly curved, tapering to apex, with short megaseta. Transverse sternapodeme triangular or trapezoidal.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFD2FFE102E7FE65D1DDFE0B.taxon	description	(Figs. 61 – 67)	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFD2FFE102E7FE65D1DDFE0B.taxon	materials_examined	Material examined. NEPAL: 1 adult male, Langtang Region, pond near Khyimjung Glacier, alt. 4175 m a. s. l., 4. VIII. 1999, leg. R. Endo. INDIA: 1 adult male Uttarakhand, Chamoli District, Rishi Ganga River, right tributary of the Alaknanda River near the Badrinath City, alt. 3128 m a. s. l., 12. V. 2018, 30.740733 N, 79.48875 E, leg. D. Palatov; 1 adult male, the same data except Alaknanda River below Badrinath City, 13. V. 2018, 30.738128 N, 79.492719 E, leg. D. Palatov.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFD2FFE102E7FE65D1DDFE0B.taxon	description	Description Adult male (n = 3, except when otherwise stated). Total length 3.2 – 3.8 mm. Total length / wing length 1.0 – 1.34. Coloration. Dark brown. Wings grayish, with brownish venation. Head. Eyes hairy, reniform. Temporal setae including 3 – 7 preoculars, 7 – 8 verticals. Clypeus with 6 – 8 setae. Antenna with 13 flagellomeres and partly reduced plume of setae (Figs. 61 – 62); maximal length of plume setae 459 μm. Length of 1 – 13 flagellomeres (n = 1) (μm): 68, 36, 36, 40, 36, 36, 40,40, 44, 36, 48, 44, 316; terminal flagellomere with 2 subapical setae, 20 – 24 μm long; AR 0.63 – 0.72. Palpomeres 1 – 5 length (μm): 36 – 40, 68 – 88, 116 – 124, 92 – 104, 115 – 164. Palpomere 3 in distal part with sensilla capitata with diameter 16 – 20 μm. Head width / palpal length 1.04 – 1.13. Thorax. Antepronotum with 2 – 8 ventrolateral setae. Dorsocentrals 5 – 7, prealars 5 – 6, scutellars 6 – 8. Wing. Length 2.8 – 3.5 mm, width 0.72 – 1.04 mm. Anal lobe round angular. Squama with 24 – 28 setae. R and R 1 with 23 – 26 setae, R 4 + 5 with 4 – 6 setae in distal part. RM / MCu 2.7 – 3.0. Legs. Spur of front tibia 60 – 98 µm long. Spurs of mid tibia 36 – 40 µm long. Spurs of hind tibia 68 – 76 µm and 36 – 40 µm long. Hind tibial comb with 14 – 16 setae. Length (μm) and proportions of leg segments are as in Table 13. Hypopygium (Figs. 63 – 67). Tergite IX with 6 – 12 weak setae, laterosternite IX with 4 – 5 setae. Anal point 188 – 204 µm long, slightly tapering to subapical (Fig. 63), 20 – 40 µm wide at base; 12 – 16 µm wide at apex; with strong anteriorly directed microtrichia in basal half and on tergite IX. Phallapodeme 92 – 112 µm long. Transverse sternapodeme (TSA) triangular, 68 – 92 µm high, 124 – 144 µm wide at the base; TSA height / TSA width 0.55 – 0.64. Gonocoxite 296 – 332 µm long; inferior volsella in the form of small lobe covered with micro- and macrotrichia and adjacent to a protuberance with some long setae (Figs. 64 – 65, 67). Superior volsella in form of small rounded angular tubercle. Gonostylus 132 – 164 µm long, slightly curved, tapering to apex, 204 µm long; megaseta 10 – 12 µm long. HR 2.02 – 2.24. Pupa and larva unknown.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
714487C0FFD2FFE102E7FE65D1DDFE0B.taxon	discussion	Remarks. Lin et al (2021) redescribed of D. loeffleri from Tibet Plateau of China by male which has a common similar structure including the hypopygium but differs of some characters (Table 14). Thus, specimens from China are larger (4.13 – 4.51 mm) than Himalayan ones, total length / wing length 1.38 – 1.43, clypeals 30 – 32, AR 0.70 – 0.82, acrostichals on the mesonotum 9 – 11, dorsocentrals 14, anal point of hypopygium length 240 – 250 µm. For males from our Himalayas material the meanings of these characters are as follows. Total length 3.2 – 3.8 mm, total length / wing length 1.0 – 1.34, clypeals 6 – 8, AR 0.63 – 0.72, acrostichals on the mesonotum absent, dorsocentrals 5 – 7, anal point of hypopygium length 188 – 204 µm. Since the number of compared males of this species from the Himalayas and Tibet Plateau is small, one would assume that this is a geographical variability, but a comparison of the barcoding data showed that these are two different species (see below), that is, the population from China, apparently, should be attributed to a new species.	en	Makarchenko, Eugenyi A., Semenchenko, Alexander A., Palatov, Dmitry M. (2022): Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae) with description and DNA barcoding of known species. II. Subgroups davisi, leona and loeffleri. Zootaxa 5190 (3): 361-392, DOI: https://doi.org/10.11646/zootaxa.5190.3.3
