taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
03AACF58FFAB1F2CFBAB1039FB449741.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184496/files/figure.png	https://doi.org/10.5281/zenodo.7184496	Figure 1. Map showing distribution of the sampled Gint species. Bottom right – G. amoudensis.	Figure 1. Map showing distribution of the sampled Gint species. Bottom right – G. amoudensis.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFAB1F2CFBAB1039FB449741.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184498/files/figure.png	https://doi.org/10.5281/zenodo.7184498	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFAA1F2EFED315A9FA929689.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184496/files/figure.png	https://doi.org/10.5281/zenodo.7184496	Figure 1. Map showing distribution of the sampled Gint species. Bottom right – G. amoudensis.	Figure 1. Map showing distribution of the sampled Gint species. Bottom right – G. amoudensis.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFAA1F2EFED315A9FA929689.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184498/files/figure.png	https://doi.org/10.5281/zenodo.7184498	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFA91F2FFBA315D2FDAE956B.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184498/files/figure.png	https://doi.org/10.5281/zenodo.7184498	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFA81F2FFED016B0FD4596BD.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184498/files/figure.png	https://doi.org/10.5281/zenodo.7184498	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFA81F20FEC415FDFD1593C0.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184498/files/figure.png	https://doi.org/10.5281/zenodo.7184498	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFA81F20FEC415FDFD1593C0.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184502/files/figure.png	https://doi.org/10.5281/zenodo.7184502	Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFA71F22FEF41709FECF94C9.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184498/files/figure.png	https://doi.org/10.5281/zenodo.7184498	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFA71F22FEF41709FECF94C9.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184502/files/figure.png	https://doi.org/10.5281/zenodo.7184502	Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFA71F22FEF41709FECF94C9.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184506/files/figure.png	https://doi.org/10.5281/zenodo.7184506	Figure 5. Bayesian tree of the Gint species based on the concatenated data set (16S and COI), complemented with summarized cytogenetic data of the species studied. Numbers above branches correspond to values for highly supported nodes as follows: Bayesian posterior probabilities (PP)> 0.95/maximum likelihood bootstrap> 70%. Specimen IDs depicted in bold indicate individuals based on chromosome counts determined for the corresponding Gint species in previous studies (see: Kovařík et al., 2013; Kovařík & Mazuch, 2015; Kovařík et al., 2018). Abbreviations: cyt, cytotype; 2n, diploid number of chromosomes; II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent.	Figure 5. Bayesian tree of the Gint species based on the concatenated data set (16S and COI), complemented with summarized cytogenetic data of the species studied. Numbers above branches correspond to values for highly supported nodes as follows: Bayesian posterior probabilities (PP)> 0.95/maximum likelihood bootstrap> 70%. Specimen IDs depicted in bold indicate individuals based on chromosome counts determined for the corresponding Gint species in previous studies (see: Kovařík et al., 2013; Kovařík & Mazuch, 2015; Kovařík et al., 2018). Abbreviations: cyt, cytotype; 2n, diploid number of chromosomes; II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
03AACF58FFA71F22FEF41709FECF94C9.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/7184508/files/figure.png	https://doi.org/10.5281/zenodo.7184508	Figure 6. Ancestral state reconstruction analyses based on the Bayesian tree from this study, depicting: A, number of chromosomes; B, position of 18S rDNA. Results of maximum parsimony analysis reconstructing ancestral states for both cytogenetic characters are visualized by colours of the circles. Arrowheads indicate hypothetical inversion on 18S rDNAbearing chromosomes.	Figure 6. Ancestral state reconstruction analyses based on the Bayesian tree from this study, depicting: A, number of chromosomes; B, position of 18S rDNA. Results of maximum parsimony analysis reconstructing ancestral states for both cytogenetic characters are visualized by colours of the circles. Arrowheads indicate hypothetical inversion on 18S rDNAbearing chromosomes.	2022-10-01	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana		Zenodo	biologists	Just, Pavel;Šťáhlavský, František;Kovařík, František;Štundlová, Jana			
