occurrenceID	taxonID	catalogNumber	collectionCode	institutionCode	typeStatus	verbatimLabel	sex	individualCount	eventDate	recordedBy	recordNumber	decimalLatitude	decimalLongitude	minimumElevationInMeters	maximumElevationInMeters	minimumDepthInMeters	maximumDepthInMeters	country	stateProvince	municipality	locality	references	associatedOccurrences	associatedReferences	basisOfRecord
03A6281BFFB5FFF0FC21F9670253FBC8.mc.3B679350FFB5FFFFFC9FF82401D4F985	03A6281BFFB5FFF0FC21F9670253FBC8.taxon	CFBH 46043		CFBH	holotype	Holotype. — CFBH 46043 (Fig. 2), an adult male collected in Brazil: State of Espírito Santo, Municipality of Castelo, Parque Estadual do Forno Grande, at Poços Amarelos (20831 0 1.21 " S, 4185 0 19.85 " W; 1,312 m) on 25 November 2021 by P. Marinho, T. R. Carvalho, and M. T. T. Santos	adult	2	2021-11-25	P. Marinho & T. R. Carvalho & M. T. T. Santos				1312				Brazil	Espirito Santo	Castelo	Parque Estadual do Forno Grande	https://treatment.plazi.org/id/03A6281BFFB5FFF0FC21F9670253FBC8#3B679350FFB5FFFFFC9FF82401D4F985			MaterialCitation
03A6281BFFB5FFF0FC21F9670253FBC8.mc.3B679350FFB2FFFFFE4FF90E01B7F945	03A6281BFFB5FFF0FC21F9670253FBC8.taxon	CFBH 46037		CFBH	paratype	CFBH 46037 – 42, collected with the holotype by the same collectors		1	2021-11-25	P. Marinho & T. R. Carvalho & M. T. T. Santos				1312				Brazil	Espirito Santo	Castelo	Parque Estadual do Forno Grande	https://treatment.plazi.org/id/03A6281BFFB5FFF0FC21F9670253FBC8#3B679350FFB2FFFFFE4FF90E01B7F945			MaterialCitation
03A6281BFFB5FFF0FC21F9670253FBC8.mc.3B679350FFB2FFFFFE2DF92F01B8F907	03A6281BFFB5FFF0FC21F9670253FBC8.taxon	AAG-UFU 6772		CFBH	paratype	AAG-UFU 6772 – 76 collected at the same locality of the holotype on 28 November 2019 by D. L. Bang, I. Vidigal, and P. Marinho		1	2019-11-28	D. L. Bang & I. Vidigal & P. Marinho				1312				Brazil	Espirito Santo	Castelo	Parque Estadual do Forno Grande	https://treatment.plazi.org/id/03A6281BFFB5FFF0FC21F9670253FBC8#3B679350FFB2FFFFFE2DF92F01B8F907			MaterialCitation
03A6281BFFB5FFF0FC21F9670253FBC8.mc.3B679350FFB2FFFFFE2BF9600273F8A2	03A6281BFFB5FFF0FC21F9670253FBC8.taxon	AAG-UFU 6798			paratype	AAG-UFU 6798 – 00 collected in the surroundings of Parque Estadual do Forno Grande (20831 0 0.46 " S, 4184 0 59.73 " W; 1,115 m), on 28 November 2019 by D. L. Bang, I. Vidigal, and P. Marinho. Diagnosis. — Boana guarinimirim sp. nov. can be distinguished from most of its congeners of the B. pulchella group by the following combination of characters: 1) dorsal pattern composed of longitudinal lines and stripes; 2) a small SVL of 24.1 – 28.1 mm (26.0 6 1.1; Table 3) in males; 3) no spots or vertical bars on hidden surfaces of legs, groin, and flanks; 4) presence of calcar appendage; 5) presence of supracloacal crest (characters 4, 5 in Fig. 3); 6) relatively conspicuous discs on fingers and toes; 7) HW 30 – 34 % (32 6 1) of SVL; 8) vocal repertoire composed of two different types of calls: the ‘‘ A' ' call (a short and multipulsed note) and the ‘‘ B' ' call (a trilled note); 9) call dominant frequency (both call types) ranging from 4.5 to 6.6 kHz (5.8 6 0.5). Morphological Comparisons. — Boana guarinimirim sp. nov. can be distinguished from all species in the B. pulchella group, except from those in the B. polytaenia clade, by the combination of the following morphological traits: the presence of dorsal pattern composed of longitudinal lines and stripes (absent in B. aguilari, B. balzani, B. caipora, B. callipleura, B. cambui, B. cordobae, B. curupi B. cymbalum, B. ericae, B. freicanecae, B. gladiator, B. joaquini, B. marginata, B. marianitae, B. melanopleura, B. palaestes, B. poaju, B. prasina, B. pulchella, B. riojana, B. semiguttata, and B. stellae); the smaller male SVL of 24.1 – 28.1 mm (B. aguilari [33.7 – 43.8 mm], B. balzani [33.3 – 49.9 mm], B. bischoffi [36.0 – 47.0 mm], B. callipleura [37.2 – 43.3 mm], B. cordobae [39.0 – 50.0 mm], B. cymbalum [44.8 – 46.2 mm], B. freicanecae [37.3 – 42.2 mm], B. gladiator [35.3 – 49.4 mm], B. goiana [29.0 – 33.0 mm], B. guentheri [33.0 – 40.0 mm], B. joaquini [40.3 – 56.4 mm], B. marginata [37.5 – 53.6 mm], B. marianitae [36.5 – 56.8 mm], B. melanopleura [43.6 – 50.0 mm], B. palaestes [36.2 – 50.4 mm], B. poaju [33.5 – 42.7 mm], B. prasina [41.0 – 47.0 mm], B. pulchella [37.0 – 49.0 mm], B. riojana [48.0 – 56.0 mm], B. semiguttata [36.1 – 45.2 mm], and B. stellae [40.7 – 49.9 mm]); and the absence of vertical bars or spots on hidden parts of thighs, flanks, or groin (present in B. aguilari, B. balzani, B. bischoffi, B. caingua, B. caipora, B. callipleura, B. cordobae, B. curupi, B. cymbalum, B. ericae, B. gladiator, B. guentheri, B. joaquini, B. marianitae, B. melanopleura, B. poaju, B. prasina, B. pulchella, B. riojana, B. semiguttata, and B. stellae; Boulenger, 1912; Bokermann, 1963; Barrio, 1965; Lutz, 1973; Heyer et al., 1990; Duellman et al., 1997; Cruz and Caramaschi, 1998, Garcia et al., 2001, 2003, 2007, 2008; Caramaschi and Cruz, 2004; Kwet, 2008; Köhler et al., 2010; Lehr et al., 2010; Pinheiro et al., 2016)., FIG. 2. Holotype of Boana guarinimirim sp. nov. (CFBH 46043) from Castelo, Parque Estadual do Forno Grande (Espírito Santo, Brazil): (A) dorsal and (B) ventral views (SVL = 24.1 mm), (C) hand and (D) foot. TABLE 3. Morphometric traits (in mm) of males of the species of the Boana polytaenia clade. Values are given as minimum – maximum (mean 6 SD). Sample sizes are in parentheses. Sources: present work, Caramaschi et al. (2009), Caramaschi and Cruz (1999), Cruz and Caramaschi (1998), Braun and Braun (1977), and Caramaschi et al. (2010). SVL HL HW ED END IND EW IOD Boana guarinimirim (15) 24.1 – 28.1 (26.0 6 1.1) 8.1 – 9.1 (8.5 6 0.3) 7.7 – 8.7 (8.2 6 0.3) 2.7 – 3.4 (3.1 6 0.2) 1.3 – 2.4 (1.8 6 0.3) 1.6 – 2.3 (1.8 6 0.2) 2.1 – 2.7 (2.3 6 0.1) 2.6 – 3.1 (2.8 6 0.1) Boana sp. 4 (5) 23.0 – 26.6 (24.5 6 1.4) 7.1 – 7.6 (7.4 6 0.2) 7.6 – 8.4 (7.9 6 0.4) 2.7 – 3.1 (2.9 6 0.2) 1.2 – 1.6 (1.5 6 0.2) 1.7 – 2.0 (1.8 6 0.1) 2.3 – 2.5 (2.4 6 0.0) 2.5 – 2.9 (2.7 6 0.2) B. polytaenia (53) 23.2 – 34.7 (27.9 6 2.3) 7.3 – 11.5 (8.9 6 0.8) 7.4 – 11.0 (8.8 6 0.7) 2.5 – 3.6 (3.0 6 0.2) 1.4 – 2.3 (1.8 6 0.2) 1.3 – 2.6 (1.9 6 0.3) 1.9 – 3.2 (2.4 6 0.2) 2.4 – 3.6 (2.8 6 0.2) B. botumirim (20) 25.9 – 31.8 (29.2 6 1.4) 8.1 – 9.9 (9.3 6 0.6) 6.4 – 9.1 (8.2 6 0.6) 2.9 – 3.5 (3.1 6 0.2) 1.7 – 2.3 (1.9 6 0.1) 2.9 – 3.5 (3.1 6 0.2) 2.2 – 2.8 (2.6 6 0.2) 2.8 – 3.3 (3.0 6 0.1) B. buriti (20) 28.2 – 31.9 (30.0 6 1.9) 8.1 – 9.0 (8.5 6 0.5) 7.5 – 8.0 (7.7 6 035) 2.6 – 3.0 (2.8 6 0.2) 1.9 – 2.0 (2.0 6 0.1) 2.4 – 2.6 (2.5 6 0.1) 1.9 – 2.0 (2.0 6 0.1) 2.7 – 3.1 (2.9 6 0.2) B. cipoensis 25.6 – 31.5 — — — — — — — B. leptolineata 27.2 – 31.6 8.7 8.6 3.2 2.0 2.6 2.0 — B. jaguariaioensis (4) 23.9 – 28.8 (26.6 6 2.0) 7.2 – 8.5 (8.1 6 0.6) 7.0 – 8.2 (7.8 6 0.5) 2.6 – 3.0 (2.9 6 0.2) 1.8 – 2.1 (2.0 6 0.1) 2.1 – 2.4 (2.2 6 0.1) 1.9 – 2.2 (2.0 6 0.1) 2.4 – 3.1 (2.8 6 0.3) B. stenocephala (17) 26.0 – 30.4 (28.3 6 1.1) 7.9 – 10.0 (8.6 6 0.7) 7.4 – 8.2 (7.7 6 0.2) 2.5 – 2.9 (2.7 6 0.1) 1.7 – 2.0 (1.8 6 0.1) 2.2 – 2.7 (2.3 6 0.1) 1.6 – 1.9 (1.7 6 0.1) 2.6 – 3.2 (2.9 6 0.2) TD TL TAL FL SL THL 3 FD 4 TD Boana guarinimirim (15) 1.0 – 1.5 (1.3 6 0.1) 12.7 – 16.2 (14.3 6 0.9) 7.6 – 9.8 (8.7 6 0.6) 10.4 – 12.9 (11.4 6 0.7) 2.5 – 3.7 (3.1 6 0.4) 10.1 – 15.3 (13.3 6 1.1) 0.9 – 1.3 (1.1 6 0.1) 0.9 – 1.2 (1.0 6 0.1) Boana sp. 4 (5) 1.3 – 1.5 (1.4 6 0.1) 12.1 – 13.7 (13.0 6 0.7) 7.6 – 8.6 (7.9 6 0.6) 9.5 – 10.8 (10.2 6 0.7) 2.1 – 2.7 (2.4 6 0.2) 11.8 – 13.3 (12.6 6 0.7) 0.8 – 1.2 (1.0 6 0.2) 1.0 – 1.2 (1.1 6 0.1) B. polytaenia (53) 1.1 – 2.1 (1.5 6 0.2) 12.3 – 17.4 (14.8 6 1.1) 7.4 – 10.5 (8.9 6 0.7) 9.5 – 15.1 (11.9 6 1.2) 2.5 – 3.8 (3.0 6 0.3) 11.8 – 17.8 (14.4 6 1.2) 0.9 – 1.5 (1.2 6 0.1) 0.9 – 1.5 (1.1 6 0.1) B. botumirim (20) 1.0 – 2.0 (1.4 6 0.3) 14.7 – 17.3 (15.7 6 0.7) — — — 13.6 – 17.2 (15.3 6 0.9) — — B. buriti (20) — 14.2 – 16.4 (15.1 6 10.2) — — — 15.0 – 16.5 (15.6 6 0.8) — — B. cipoensis — — — — — — — — B. leptolineata 1.3 15.2 — — — — — — B. jaguariaioensis (4) 1.2 – 1.7 (1.5 6 0.2) 11.5 – 14.3 (13.1 6 1.2) — — — 11.9 – 14.2 (13.2 6 1.0) 0.7 – 0.8 (0.8 6 0.0) 0.5 – 0.9 (0.7 6 0.1) B. stenocephala (17) — 13.0 – 15.0 (14.0 6 00.6) — — — 13.5 – 16.0 (14.7 6 0.6) — — From species of the B. polytaenia clade, B. guarinimirim sp. nov. can be distinguished by the presence of a calcar appendage and a distinct supracloacal crest (Fig. 3, absent in B. botumirim, B. buriti, B. cipoensis, B. jaguariaioensis, B. leptolineata, and B. stenocephala); relatively more conspicuous discs on fingers and toes (less conspicuous in B. botumirim, B. jaguariaioensis, and B. stenocephala), and the HW of 30 – 34 % of SVL (mean of 27 % in B. botumirim, 26 % in B. buriti, 29 % in B. jaguariaioensis, and 27 % in B. stenocephala). The male SVL of 24.1 to 28.1 mm of B. guarinimirim sp. nov. is smaller than B. buriti (28.2 – 31.9 mm; Lutz, 1968; Braun and Braun, 1977; Cruz and Caramaschi, 1998; Caramaschi and Cruz, 1999; Caramaschi et al., 2009; Caramaschi et al., 2010). B. guarinimirim cannot be distinguished from B. polytaenia and Boana sp. 4 in any of the analyzed morphological traits Vocal Repertoire. — The vocal repertoire of B. guarinimirim sp. nov. (Fig. 4 A-B; Table 4) is composed of two types of calls, referred to as the ‘‘ A' ' and ‘‘ B' ' calls. The ‘‘ A' ' call is formed by a single and short multipulsed note and is commonly emitted alone or followed by a ‘‘ B' ' call but can also be emitted in pairs or in a series of consecutive ‘‘ A' ' calls. It lasts 18 – 84 ms (48 6 14) and reaches the amplitude peak at 1 % to 76 % (20 6 17) of its total duration. Pulses can be either well defined (with complete amplitude modulation, n = 27 calls) or poorly defined (concatenated pulses, n = 117 calls) along the call. In calls with well-defined pulses, the pulse rate ranges from 178 to 310 pulses per second (243 6 28), with 5 – 14 pulses per call. The dominant frequency always corresponds to the second harmonic and ranges from 4.6 to 6.6 kHz (6.2 6 0.6). The minimum frequency ranges from 4.4 to 6.2 kHz (5.4 6 0.5) and the maximum frequency from 5.0 to 7.2 kHz (6.3 6 0.6). The ‘‘ B' ' call is less common, and is usually preceded by an ‘‘ A' ' call, but can be emitted alone or followed by an ‘‘ A' ' call. The ‘‘ B' ' call lasts 381 – 915 ms (574 6 136) and is composed of 5 – 11 pulses, which are emitted at irregular intervals of 2 – 284 ms (74 6 36). Pulses have durations of 2 – 16 ms (8 6 3). The ‘‘ B' ' call reaches its amplitude peak at 1 – 97 % (48 6 35) of the total call duration. The dominant frequency corresponds to the second visible harmonic and ranges from 4.5 to 6.3 kHz (5.4 6 0.5). The minimum frequency ranges from 3.5 to 5.9 kHz (5.0 6 0.6) and the maximum frequency from 4.7 to 6.8 kHz (5.8 6 0.6).. FIG. 3. Comparison of (A) Boana guarinimirim sp. nov. with other species in the B. polytaenia clade: (B) B. cipoensis (AAG-UFU 0027), (C) B. buriti (AAG-UFU 0433), and (D) B. stenocephala (AAG-UFU 4827). Arrows in (A) indicate the supracloacal crest and the calcar appendage, absent in B – D. Specimens are not to scale. FIG. 4. Advertisement calls (spectrograms and corresponding oscillograms) of (A – B) B. guarinimirim sp. nov. (sound files: Boana _ guarinimirimFornoGrandeES 2 aPM _ AAGm 671 and Boana _ guarinimirimFornoGrandeES 3 aPM _ AAGm 671, respectively); (C – D) Boana sp. 4 (sound file: Boana _ sp. 4 BJAmparoMG 3 aAAGm 671); and (E – F) B. polytaenia (sound file: Boana _ polytaeniaNovFriburRJ 1 bLBM _ AAGmt). See Appendix 2 for additional information on sound files. Acoustic Comparisons. — The call of Boana guarinimirim sp. nov. can be distinguished from the call of B. caingua, B. callipleura, B. cambui, B. cordobae, B. freicanecae, B. goiana, B. melanopleura, B. pulchella, and B. riojana by being pulsed (nonpulsed in those species; Baraquet et al., 2007, 2014; Köhler et al., 2010; Lehr et al., 2010; Pinheiro et al., 2012; Batista et al., 2015; Furtado et al., 2016; Pinheiro et al., 2016; Marinho et al., 2020). Species of the B. pulchella group with pulsed calls, except those in the B. polytaenia clade, are B. aguilari, B. balzani, B. bischoffi, B. caipora, B. callipleura, B. curupi, B. ericae, B. gladiator, B. guentheri, B. joaquini, B. marginata, B. marianitae, B. palaestes, B. poaju, B. prasina, B. semiguttata, and B. stellae. The advertisement call of B. guarinimirim can be distinguished from those species, except from B. bischoffi and B. ericae, by being composed of two types of calls (a single and pulsed call, or a series of similar calls in those species; Duellman et al., 1997; Garcia et al., 2001, 2003, 2007, 2008; Antunes et al., 2008; Garcia and Haddad, 2008; Kwet, 2008; Köhler et al., 2010; Lehr et al., 2010; Pombal, 2010; Delgado and Haddad, 2015; Forti et al., 2019). From B. bischoffi and B. ericae, the calls of B. guarinimirim can be distinguished by its higher dominant frequency of 4.5 – 6.6 kHz (1.4 – 2.1 kHz in calls of B. bischoffi and 2.0 – 3.6 kHz in calls of B. ericae; Garcia and Haddad, 2008; Pombal, 2010). Additionally, the vocal repertoire of B. ericae is composed of three types of calls: calls ‘‘ A, '' ‘‘ B, '' and ‘‘ C. '' The ‘‘ C' ' call consists of a trill of pulses similar to the ‘‘ B' ' call of B. guarinimirim sp. nov. and can be distinguished from it by the longer duration of 381 – 915 ms in the ‘‘ B' ' call of B. guarinimirim sp. nov. (‘‘ C' ' call duration of 104 – 560 ms in B. ericae). Among species within the Boana polytaenia clade, the calls of B. buriti and B. cipoensis remains undescribed. The calls of B. guarinimirim sp. nov. can be distinguished from the calls of B. botumirim and B. stenocephala by the higher dominant frequency of 4.5 – 6.6 kHz (dominant frequency of 3.3 – 4.1 kHz in ‘‘ A' ' calls of B. botumirim and B. stenocephala; Caramaschi et al., 2009; Martins et al., 2016) and by being composed of two distinct call types (‘‘ B' ' call unknown in B. botumirim). Also, the call of B. guarinimirim can be distinguished from the calls of B. jaguariaioensis and B. stenenocephala by the ‘‘ A' ' call being composed of only one note (‘‘ A' ' call composed by a sequence of short notes in B. jaguariaioensis and B. stenocephala; Martins et al., 2016; Guerra et al., 2017). The described calls of B. leptolineata (Forti et al., 2019) cannot be distinguished from the calls of B. guarinimirim based on any of the analyzed traits; however, these species are easily distinguished by morphological features and are not closely related (Faivovich et al., 2021). Although the DAPC analysis pointed to a discrimination between B. guarinimirim and its distant taxon B. polytaenia (Fig. 1 B), we could not find any acoustic trait that did not overlap in our analysis (Table 4). Additionally, B. guarinimirim could not be distinguished from Boana sp. 4 based on acoustic traits.		1	2019-11-28	D. L. Bang & I. Vidigal & P. Marinho				1115							Parque Estadual do Forno Grande	https://treatment.plazi.org/id/03A6281BFFB5FFF0FC21F9670253FBC8#3B679350FFB2FFFFFE2BF9600273F8A2			MaterialCitation
