identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
C82A87F6D445F32BFE23FD92378FFBC8.text	C82A87F6D445F32BFE23FD92378FFBC8.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Saccharomyces cerevisiae	<div><p>2.6. Functional characterization of Aa7DR 1 in  S. cerevisiae</p><p>Yeast lacks the 7DR gene, and Δ 5,7 -eposterol can be used as a substrate for the heterologous 7DR enzyme (Fig. 1). The two Aa7DRs were heterologously expressed in  S. cerevisiae for functional characterization. Aa7DR1 and Aa7DR2 were cloned into the expression vectors pESC-HIS-Aa7DR1 and pESC-HIS-Aa7DR2, then were transformed into  S. cerevisiae strain Cen.pk2-1D. Compared with the empty-vector-transferred control, the yeast strain expressing Aa7DR1 yielded two prominent products (peaks 1 and 2) at comparable levels (Fig. 6A). While there were no significant differences between pESC-His-Aa7DR2 and the control. To determine the structural features of Aa7DR1 products a large-scale fermentation (7 L) was used to accumulate enough products. The products were isolated and purified using open silica gel column and semi-preparative reversed-phase high performance liquid chromatography. The structures of p1 were confirmed as ergosta-5,22-dien-3β- ol by 1 H and 13 C-NMR data (Supplemental Figures S6 and S 7; Supplemental Table S6). Product p2 was identified as campesterol by 1 H-NMR and comparison with the standard. We determined Aa7DR1 possessed 7- dehydrocholesterol reductive function to reduce Δ 5,7 -eposterol and ergosterol at Δ 7 position. The indirect product campesterol (p2) was further catalyzed by endogenous gene erg4 on ostreasterol (Fig. 5).</p></div>	https://treatment.plazi.org/id/C82A87F6D445F32BFE23FD92378FFBC8	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Wang, Yunpeng;Xiong, Wenbo;Chen, Yidu;Zhu, Min;Liang, Jincai;Li, Yu;Huang, Jia;Huang, Liufang;Liu, Zhongqiu;Ji, Aijia;Duan, Lixin	Wang, Yunpeng, Xiong, Wenbo, Chen, Yidu, Zhu, Min, Liang, Jincai, Li, Yu, Huang, Jia, Huang, Liufang, Liu, Zhongqiu, Ji, Aijia, Duan, Lixin (2021): Transcriptomic investigation of the biochemical function of 7 - dehydro- cholesterol reductase 1 from the traditional Chinese medicinal plant Anemarrhena asphodeloides Bunge. Phytochemistry (112954) 192: 1-9, DOI: 10.1016/j.phytochem.2021.112954, URL: http://dx.doi.org/10.1016/j.phytochem.2021.112954
