taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
03EBF734A03016240125F9B0FD84F8AD.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8258214/files/figure.png	https://doi.org/10.5281/zenodo.8258214	Fig. 1. The identification of volatiles emitted from the flowers of V. cruziana. A, the chromatogram of the volatile emission from the flower during the first bloom. The four peaks were identified as methyl hexanoate (peak 1), benzyl alcohol (peak 2), benzyl 2-methylbutanoate (peak 3), and benzyl tiglate (peak 4). IS stands for internal standard, nonyl acetate. B, chromatogram of three authentic compounds. Peak a1: methyl hexanoate; peak a2: benzyl alcohol (peak 2); peak a3: benzyl tiglate. C. mass spectrum of three compounds from flowers (peaks 1, 2 and 4) and their corresponding authentic standard (peaks a1, a2 and a3).	Fig. 1. The identification of volatiles emitted from the flowers of V. cruziana. A, the chromatogram of the volatile emission from the flower during the first bloom. The four peaks were identified as methyl hexanoate (peak 1), benzyl alcohol (peak 2), benzyl 2-methylbutanoate (peak 3), and benzyl tiglate (peak 4). IS stands for internal standard, nonyl acetate. B, chromatogram of three authentic compounds. Peak a1: methyl hexanoate; peak a2: benzyl alcohol (peak 2); peak a3: benzyl tiglate. C. mass spectrum of three compounds from flowers (peaks 1, 2 and 4) and their corresponding authentic standard (peaks a1, a2 and a3).	2021-11-30	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng		Zenodo	biologists	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng			
03EBF734A03016240125F9B0FD84F8AD.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8258216/files/figure.png	https://doi.org/10.5281/zenodo.8258216	Fig. 2. Emission of floral volatiles from different of parts of V. cruziana flowers. Intact fully opened flowers were separated into petals, pistils, sepals and stamen, which were subject to headspace collection and GC-MS analysis. In addition to total volatiles (VOCs), the emissions of methyl hexanoate and benzenoids were analyzed separately.	Fig. 2. Emission of floral volatiles from different of parts of V. cruziana flowers. Intact fully opened flowers were separated into petals, pistils, sepals and stamen, which were subject to headspace collection and GC-MS analysis. In addition to total volatiles (VOCs), the emissions of methyl hexanoate and benzenoids were analyzed separately.	2021-11-30	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng		Zenodo	biologists	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng			
03EBF734A03016240125F9B0FD84F8AD.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8258218/files/figure.png	https://doi.org/10.5281/zenodo.8258218	Fig. 3. Emission dynamic of floral volatiles from V. cruziana flowers during two consecutive days of blooming and closing. A, representative flower at four stages during blooming. B, the emission dynamics of total volatiles. C, the emission dynamcis of benzenoids. D. the emission dynamics of methyl hexanoate. Different letters denote statistically significant differences among the means according to ANOVA analysis (P <0.05).	Fig. 3. Emission dynamic of floral volatiles from V. cruziana flowers during two consecutive days of blooming and closing. A, representative flower at four stages during blooming. B, the emission dynamics of total volatiles. C, the emission dynamcis of benzenoids. D. the emission dynamics of methyl hexanoate. Different letters denote statistically significant differences among the means according to ANOVA analysis (P <0.05).	2021-11-30	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng		Zenodo	biologists	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng			
03EBF734A03016240125F9B0FD84F8AD.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8258220/files/figure.png	https://doi.org/10.5281/zenodo.8258220	Fig. 4. Multiple sequence alignment of VcSABATHs with selected known SABATHs. Conserved residues are in shade with the more conserved the darker. Residues indicated with “&” are S-adenosyl-L-methionine-binding residues. Residues indicated with “*” are residues that interact with the carboxyl moiety of substrate. CbSAMT, Clarkia breweri salicylic acid methyltransferase (accession No. AAF00108.1); NcDEMT, Nymphaea colorata decanoic acid methyltransferase (accession No. NC11G0120830).	Fig. 4. Multiple sequence alignment of VcSABATHs with selected known SABATHs. Conserved residues are in shade with the more conserved the darker. Residues indicated with “&” are S-adenosyl-L-methionine-binding residues. Residues indicated with “*” are residues that interact with the carboxyl moiety of substrate. CbSAMT, Clarkia breweri salicylic acid methyltransferase (accession No. AAF00108.1); NcDEMT, Nymphaea colorata decanoic acid methyltransferase (accession No. NC11G0120830).	2021-11-30	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng		Zenodo	biologists	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng			
03EBF734A03016240125F9B0FD84F8AD.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8258222/files/figure.png	https://doi.org/10.5281/zenodo.8258222	Fig. 5. GC chromatogram of product of methyl- transferase enzyme assays for VcSABATH1-3. The assay conducted with proteins expressed in E. coli with pET32a without any gene insert (empty vector) was used as a negative control. Also shown was the GC chromatogram of the authentic standard methyl hexanoate. Hexanoic acid was used as substrate. While no product was detected from the VcSABATH2 assay, both VcSABATH1 and VcSABATH3 catalyzed the formation of methyl hexanoate (peak 1).	Fig. 5. GC chromatogram of product of methyl- transferase enzyme assays for VcSABATH1-3. The assay conducted with proteins expressed in E. coli with pET32a without any gene insert (empty vector) was used as a negative control. Also shown was the GC chromatogram of the authentic standard methyl hexanoate. Hexanoic acid was used as substrate. While no product was detected from the VcSABATH2 assay, both VcSABATH1 and VcSABATH3 catalyzed the formation of methyl hexanoate (peak 1).	2021-11-30	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng		Zenodo	biologists	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng			
03EBF734A03016240125F9B0FD84F8AD.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8258224/files/figure.png	https://doi.org/10.5281/zenodo.8258224	Fig. 6. Gene expression pattern of VcSABATH1 (A) and VcSABATH3 (B) in four different parts of V. cruziana flowers. Gene transcript levels were measured using RT-qPCR with VcGADPH (glyceraldehyde-3-phosphate dehydrogenase) gene as the internal control. The reactions were performed with three biological repeats, and the data was calculated by 2 ΔΔCT method. The highest levels of expression for each gene were arbitrarily set as 1.0. Different letters denote statistically significant differences among the means according to ANOVA analysis (P <0.05).	Fig. 6. Gene expression pattern of VcSABATH1 (A) and VcSABATH3 (B) in four different parts of V. cruziana flowers. Gene transcript levels were measured using RT-qPCR with VcGADPH (glyceraldehyde-3-phosphate dehydrogenase) gene as the internal control. The reactions were performed with three biological repeats, and the data was calculated by 2 ΔΔCT method. The highest levels of expression for each gene were arbitrarily set as 1.0. Different letters denote statistically significant differences among the means according to ANOVA analysis (P <0.05).	2021-11-30	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng		Zenodo	biologists	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng			
03EBF734A03016240125F9B0FD84F8AD.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8258226/files/figure.png	https://doi.org/10.5281/zenodo.8258226	Fig. 7. The phylogenetic analysis of VcSABATHs with the SABATH genes identified from N. corolata, non-seed and model species. 39 full-length proteins starting with “Os” are from rice, 24 proteins starting with “At” are from Arabidopsis, seven full-length proteins starting with “NC” are from Nymphaea colorata, five proteins starting with “Pa” are from Picea abies, three proteins starting with “Pt” are from poplar and three proteins starting with “Vc” are from V. cruziana. IAMT: indole-3-acetic acid MT; SAMT: salicylic acid MT; JAMT: jasmonic acid MT; GAMT: gibberellic acid MT; BSMT: benzoic acid/salicylic acid MT; FAMT: farnesoic acid MT. PpSABATH1 from the moss Physcomitrella patterns (Zhao et al., 2012) was used as an outgroup. Bootstrap values of 50 % or higher are indicated. The water lily-specific cluster was shaded.	Fig. 7. The phylogenetic analysis of VcSABATHs with the SABATH genes identified from N. corolata, non-seed and model species. 39 full-length proteins starting with “Os” are from rice, 24 proteins starting with “At” are from Arabidopsis, seven full-length proteins starting with “NC” are from Nymphaea colorata, five proteins starting with “Pa” are from Picea abies, three proteins starting with “Pt” are from poplar and three proteins starting with “Vc” are from V. cruziana. IAMT: indole-3-acetic acid MT; SAMT: salicylic acid MT; JAMT: jasmonic acid MT; GAMT: gibberellic acid MT; BSMT: benzoic acid/salicylic acid MT; FAMT: farnesoic acid MT. PpSABATH1 from the moss Physcomitrella patterns (Zhao et al., 2012) was used as an outgroup. Bootstrap values of 50 % or higher are indicated. The water lily-specific cluster was shaded.	2021-11-30	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng		Zenodo	biologists	Jiang, Yifan;Liu, Guanhua;Zhang, Wanbo;Zhang, Chi;Chen, Xinlu;Chen, Yuchu;Yu, Cuiwei;Yu, Dongbei;Fu, Jianyu;Chen, Feng			
