taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349636/files/figure.png	https://doi.org/10.5281/zenodo.8349636	FIGURE 8 Shells of Semisulcospira niponica and S. fuscata. A–F,Lectotype (A–C), NHM 1875.11.24.11/1, paralectotypes (D, E), NHM 1875.11.24.11/2,1875.11.24.11/3 and the label of S.niponica (F). G–K.Holotype of S.biwae, SMF 291511.L–P, Holotype of S.fuscata, LBM 13-1. Q–S, S.niponica from Oura Port, KUZ Z4091.T–W, S.niponica from Nagahama Port, KUZ Z4093.X–Z, S.niponica from Iso, KUZ Z4095. AA–AC, S.niponica from Kitakomatsu Port, KUZ Z4097. AD–AE, S.niponica from Kitakomatsu Beach, KUZ Z4098.AF–AH, S. niponica from Katata Port, KUZ Z4099.AI–AK, S. niponica from Otsu Port, KUZ Z3766.AL–AN, S.niponica from Nango, KUZ Z4101.AO–AQ, S.fuscata from Lake Yogo, KUZ Z4103. AR–AT, S.fuscata from Oura, KUZ Z4105.AU–AX, S.fuscata from Nihonmatsu, KUZ Z4107.AY–BA, Putative hybrid between S.fuscata and S.watanabei sp. nov.from Nihonmatsu, KUZ Z4140.A–E, G–I, L–N, Q, T, X, AA, AD, AF, AI, AL, AO, AR, AU, AY, Adult shell.R, U, Y, AB, AE, AG, AJ, AM, AP, AS, AW, AZ, Operculum.S, W, Z, AC, AH, AK, AN, AQ, AT, AX, BA, Embryonic shell. F,J, K, O, P, Label. Scale bars: 10 mm (A–E, G–I, L–N,Q, R, T,U, X,Y,AA, AB, AD–AG, AI, AJ, AL, AM, AO, AP, AR, AS, AU, AW, AY, AZ), 1 mm (S, W,Z, AC, AH, AK, AN, AQ, AT, AX, BA). Newly collected specimens (Q–BA) were treated with 3% sodium hypochlorite	FIGURE 8 Shells of Semisulcospira niponica and S. fuscata. A–F,Lectotype (A–C), NHM 1875.11.24.11/1, paralectotypes (D, E), NHM 1875.11.24.11/2,1875.11.24.11/3 and the label of S.niponica (F). G–K.Holotype of S.biwae, SMF 291511.L–P, Holotype of S.fuscata, LBM 13-1. Q–S, S.niponica from Oura Port, KUZ Z4091.T–W, S.niponica from Nagahama Port, KUZ Z4093.X–Z, S.niponica from Iso, KUZ Z4095. AA–AC, S.niponica from Kitakomatsu Port, KUZ Z4097. AD–AE, S.niponica from Kitakomatsu Beach, KUZ Z4098.AF–AH, S. niponica from Katata Port, KUZ Z4099.AI–AK, S. niponica from Otsu Port, KUZ Z3766.AL–AN, S.niponica from Nango, KUZ Z4101.AO–AQ, S.fuscata from Lake Yogo, KUZ Z4103. AR–AT, S.fuscata from Oura, KUZ Z4105.AU–AX, S.fuscata from Nihonmatsu, KUZ Z4107.AY–BA, Putative hybrid between S.fuscata and S.watanabei sp. nov.from Nihonmatsu, KUZ Z4140.A–E, G–I, L–N, Q, T, X, AA, AD, AF, AI, AL, AO, AR, AU, AY, Adult shell.R, U, Y, AB, AE, AG, AJ, AM, AP, AS, AW, AZ, Operculum.S, W, Z, AC, AH, AK, AN, AQ, AT, AX, BA, Embryonic shell. F,J, K, O, P, Label. Scale bars: 10 mm (A–E, G–I, L–N,Q, R, T,U, X,Y,AA, AB, AD–AG, AI, AJ, AL, AM, AO, AP, AR, AS, AU, AW, AY, AZ), 1 mm (S, W,Z, AC, AH, AK, AN, AQ, AT, AX, BA). Newly collected specimens (Q–BA) were treated with 3% sodium hypochlorite	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349640/files/figure.png	https://doi.org/10.5281/zenodo.8349640	FIGURE 10 Shells of Semisulcospira nakanoi sp. nov. A–G,Holotype, KUZ Z4122.H–J, Paratype, KUZ Z4125. K–L, Paratype, KUZ Z4126.M–Q, Specimens from Onoe Port, KUZ Z4129, Z4130.A–C, H, K, M, P, Adult shell. A–C, H, M, Female.K, P,Male.D, I, L, N, Q, Operculum.E–G, J, O, Embryonic shell.Scale bars: 10 mm (A–D, H–I, K–L, M–N, P–Q), 1 mm (E–G, J, O). All specimens were treated with 3% sodium hypochlorite	FIGURE 10 Shells of Semisulcospira nakanoi sp. nov. A–G,Holotype, KUZ Z4122.H–J, Paratype, KUZ Z4125. K–L, Paratype, KUZ Z4126.M–Q, Specimens from Onoe Port, KUZ Z4129, Z4130.A–C, H, K, M, P, Adult shell. A–C, H, M, Female.K, P,Male.D, I, L, N, Q, Operculum.E–G, J, O, Embryonic shell.Scale bars: 10 mm (A–D, H–I, K–L, M–N, P–Q), 1 mm (E–G, J, O). All specimens were treated with 3% sodium hypochlorite	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349626/files/figure.png	https://doi.org/10.5281/zenodo.8349626	FIGURE 5 Phylogenetic relationships of five Semisulcospira species estimated by the Neighbor-Net reconstructed based on uncorrected p-distances of 738 SNPs	FIGURE 5 Phylogenetic relationships of five Semisulcospira species estimated by the Neighbor-Net reconstructed based on uncorrected p-distances of 738 SNPs	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349630/files/figure.png	https://doi.org/10.5281/zenodo.8349630	FIGURE 6 Map of Lake Biwa indicating geographical variation in the frequency of sculpture types in the adult females of five Semisulcospira species.Colours of locality names correspond to the colour coding in fig.1	FIGURE 6 Map of Lake Biwa indicating geographical variation in the frequency of sculpture types in the adult females of five Semisulcospira species.Colours of locality names correspond to the colour coding in fig.1	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349632/files/figure.png	https://doi.org/10.5281/zenodo.8349632	FIGURE 7 Results of Random Forest analyses conducted for five Semisulcospira species. Euclidean distances generated from proximities among individuals are plotted. A, Female.B, Male	FIGURE 7 Results of Random Forest analyses conducted for five Semisulcospira species. Euclidean distances generated from proximities among individuals are plotted. A, Female.B, Male	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349620/files/figure.png	https://doi.org/10.5281/zenodo.8349620	FIGURE 3 Results of principal components analysis based on 738 SNPs conducted for five Semisulcospira species. A, Principal component (PC) 1 vs PC2.B, PC2 vs PC3. C, PC3 vs PC4. D, PC4 vs PC5	FIGURE 3 Results of principal components analysis based on 738 SNPs conducted for five Semisulcospira species. A, Principal component (PC) 1 vs PC2.B, PC2 vs PC3. C, PC3 vs PC4. D, PC4 vs PC5	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349638/files/figure.png	https://doi.org/10.5281/zenodo.8349638	FIGURE 9 Shells of Semisulcospira watanabei sp. nov.A–G, Holotype, KUZ Z4109. H–J, Paratype, KUZ Z4110.K–L, Paratype, KUZ Z4114.M–N.Specimen from Oura, KUZ Z4117.O–Q, Specimen from Nihonmatsu, KUZ Z4118.R–T, Specimen from Horikiri Port, KUZ Z4120.A–C, H, K, M, O, R, Adult shell.A–C, H, O, R, Female.K, Male. M, Juvenile.D, I, L, N, P, S, Operculum. E–G, J, Q,T, Embryonic shell. Scale bars: 10mm (A–D, H–I, K–L, M–N, O–P, R–S), 1 mm (E–G, J, Q, T). All specimens were treated with 3% sodium hypochlorite	FIGURE 9 Shells of Semisulcospira watanabei sp. nov.A–G, Holotype, KUZ Z4109. H–J, Paratype, KUZ Z4110.K–L, Paratype, KUZ Z4114.M–N.Specimen from Oura, KUZ Z4117.O–Q, Specimen from Nihonmatsu, KUZ Z4118.R–T, Specimen from Horikiri Port, KUZ Z4120.A–C, H, K, M, O, R, Adult shell.A–C, H, O, R, Female.K, Male. M, Juvenile.D, I, L, N, P, S, Operculum. E–G, J, Q,T, Embryonic shell. Scale bars: 10mm (A–D, H–I, K–L, M–N, O–P, R–S), 1 mm (E–G, J, Q, T). All specimens were treated with 3% sodium hypochlorite	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349616/files/figure.png	https://doi.org/10.5281/zenodo.8349616	FIGURE 1 Map of Lake Biwa (A) and Oura Bay (B) showing 15 sampling localities:blue, Semisulcospira niponica; green, S.fuscata; purple, S.watanabei sp.nov.; red, S.nakanoi sp.nov.; orange, S.salebrosa sp.nov; black, putative hybrid between S.fuscata and S.watanabei sp.nov.	FIGURE 1 Map of Lake Biwa (A) and Oura Bay (B) showing 15 sampling localities:blue, Semisulcospira niponica; green, S.fuscata; purple, S.watanabei sp.nov.; red, S.nakanoi sp.nov.; orange, S.salebrosa sp.nov; black, putative hybrid between S.fuscata and S.watanabei sp.nov.	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349618/files/figure.png	https://doi.org/10.5281/zenodo.8349618	FIGURE 2 Schematic drawings representing shell measurements of Semisulcospira species in this study. A, Spire angle (SA) and aperture swell length (ASL) of adult shell. B–D, Criteria for types of sculptures	FIGURE 2 Schematic drawings representing shell measurements of Semisulcospira species in this study. A, Spire angle (SA) and aperture swell length (ASL) of adult shell. B–D, Criteria for types of sculptures	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349624/files/figure.png	https://doi.org/10.5281/zenodo.8349624	FIGURE 4 Results of ADMIXTURE analysis based on 738 SNPs conducted for five Semisulcospira species.Bar colours of the species in K = 5 correspond to the colour coding in fig.3	FIGURE 4 Results of ADMIXTURE analysis based on 738 SNPs conducted for five Semisulcospira species.Bar colours of the species in K = 5 correspond to the colour coding in fig.3	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFFFAD5938DFF66FECD8C85C.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349644/files/figure.png	https://doi.org/10.5281/zenodo.8349644	FIGURE 12 Radulae of Semisulcospira species.A–G, Semisulcospira niponica: A, Oura Port, KUZ Z4091; B, Nagahama Port, KUZ Z4093; C, Iso, KUZ Z4095; D, Kitakomatsu Port, KUZ Z4097; E, Katata Port, KUZ Z4099; F, Otsu Port, KUZ Z4101; G, Nango, KUZ Z4103.H–J, Semisulcospira fuscata: H, Lake Yogo, KUZ Z4103; I, Oura, KUZ Z4105; J, Nihonmatsu, KUZ Z4107.K–L, Semisulcospira watanabei sp. nov.:K, Kitakomatsu Port, KUZ Z4115;L, Horikiri Port, KUZ Z4120.M–N, Semisulcospira nakanoi sp. nov.: M, Onoe Port, KUZ Z4129;N, Chikubu-shima Island, KUZ Z4127.O–P, Semisulcospira salebrosa sp. nov.:O, Take-shima Island, KUZ Z4138;P, Shiraishi-jima Island, KUZ Z4136.Scale bars:100 Μm	FIGURE 12 Radulae of Semisulcospira species.A–G, Semisulcospira niponica: A, Oura Port, KUZ Z4091; B, Nagahama Port, KUZ Z4093; C, Iso, KUZ Z4095; D, Kitakomatsu Port, KUZ Z4097; E, Katata Port, KUZ Z4099; F, Otsu Port, KUZ Z4101; G, Nango, KUZ Z4103.H–J, Semisulcospira fuscata: H, Lake Yogo, KUZ Z4103; I, Oura, KUZ Z4105; J, Nihonmatsu, KUZ Z4107.K–L, Semisulcospira watanabei sp. nov.:K, Kitakomatsu Port, KUZ Z4115;L, Horikiri Port, KUZ Z4120.M–N, Semisulcospira nakanoi sp. nov.: M, Onoe Port, KUZ Z4129;N, Chikubu-shima Island, KUZ Z4127.O–P, Semisulcospira salebrosa sp. nov.:O, Take-shima Island, KUZ Z4138;P, Shiraishi-jima Island, KUZ Z4136.Scale bars:100 Μm	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF5AD5A38DFF162EF88C909.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349638/files/figure.png	https://doi.org/10.5281/zenodo.8349638	FIGURE 9 Shells of Semisulcospira watanabei sp. nov.A–G, Holotype, KUZ Z4109. H–J, Paratype, KUZ Z4110.K–L, Paratype, KUZ Z4114.M–N.Specimen from Oura, KUZ Z4117.O–Q, Specimen from Nihonmatsu, KUZ Z4118.R–T, Specimen from Horikiri Port, KUZ Z4120.A–C, H, K, M, O, R, Adult shell.A–C, H, O, R, Female.K, Male. M, Juvenile.D, I, L, N, P, S, Operculum. E–G, J, Q,T, Embryonic shell. Scale bars: 10mm (A–D, H–I, K–L, M–N, O–P, R–S), 1 mm (E–G, J, Q, T). All specimens were treated with 3% sodium hypochlorite	FIGURE 9 Shells of Semisulcospira watanabei sp. nov.A–G, Holotype, KUZ Z4109. H–J, Paratype, KUZ Z4110.K–L, Paratype, KUZ Z4114.M–N.Specimen from Oura, KUZ Z4117.O–Q, Specimen from Nihonmatsu, KUZ Z4118.R–T, Specimen from Horikiri Port, KUZ Z4120.A–C, H, K, M, O, R, Adult shell.A–C, H, O, R, Female.K, Male. M, Juvenile.D, I, L, N, P, S, Operculum. E–G, J, Q,T, Embryonic shell. Scale bars: 10mm (A–D, H–I, K–L, M–N, O–P, R–S), 1 mm (E–G, J, Q, T). All specimens were treated with 3% sodium hypochlorite	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF5AD5A38DFF162EF88C909.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349644/files/figure.png	https://doi.org/10.5281/zenodo.8349644	FIGURE 12 Radulae of Semisulcospira species.A–G, Semisulcospira niponica: A, Oura Port, KUZ Z4091; B, Nagahama Port, KUZ Z4093; C, Iso, KUZ Z4095; D, Kitakomatsu Port, KUZ Z4097; E, Katata Port, KUZ Z4099; F, Otsu Port, KUZ Z4101; G, Nango, KUZ Z4103.H–J, Semisulcospira fuscata: H, Lake Yogo, KUZ Z4103; I, Oura, KUZ Z4105; J, Nihonmatsu, KUZ Z4107.K–L, Semisulcospira watanabei sp. nov.:K, Kitakomatsu Port, KUZ Z4115;L, Horikiri Port, KUZ Z4120.M–N, Semisulcospira nakanoi sp. nov.: M, Onoe Port, KUZ Z4129;N, Chikubu-shima Island, KUZ Z4127.O–P, Semisulcospira salebrosa sp. nov.:O, Take-shima Island, KUZ Z4138;P, Shiraishi-jima Island, KUZ Z4136.Scale bars:100 Μm	FIGURE 12 Radulae of Semisulcospira species.A–G, Semisulcospira niponica: A, Oura Port, KUZ Z4091; B, Nagahama Port, KUZ Z4093; C, Iso, KUZ Z4095; D, Kitakomatsu Port, KUZ Z4097; E, Katata Port, KUZ Z4099; F, Otsu Port, KUZ Z4101; G, Nango, KUZ Z4103.H–J, Semisulcospira fuscata: H, Lake Yogo, KUZ Z4103; I, Oura, KUZ Z4105; J, Nihonmatsu, KUZ Z4107.K–L, Semisulcospira watanabei sp. nov.:K, Kitakomatsu Port, KUZ Z4115;L, Horikiri Port, KUZ Z4120.M–N, Semisulcospira nakanoi sp. nov.: M, Onoe Port, KUZ Z4129;N, Chikubu-shima Island, KUZ Z4127.O–P, Semisulcospira salebrosa sp. nov.:O, Take-shima Island, KUZ Z4138;P, Shiraishi-jima Island, KUZ Z4136.Scale bars:100 Μm	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF5AD5A38DFF162EF88C909.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349616/files/figure.png	https://doi.org/10.5281/zenodo.8349616	FIGURE 1 Map of Lake Biwa (A) and Oura Bay (B) showing 15 sampling localities:blue, Semisulcospira niponica; green, S.fuscata; purple, S.watanabei sp.nov.; red, S.nakanoi sp.nov.; orange, S.salebrosa sp.nov; black, putative hybrid between S.fuscata and S.watanabei sp.nov.	FIGURE 1 Map of Lake Biwa (A) and Oura Bay (B) showing 15 sampling localities:blue, Semisulcospira niponica; green, S.fuscata; purple, S.watanabei sp.nov.; red, S.nakanoi sp.nov.; orange, S.salebrosa sp.nov; black, putative hybrid between S.fuscata and S.watanabei sp.nov.	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF5AD5A38DFF162EF88C909.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349626/files/figure.png	https://doi.org/10.5281/zenodo.8349626	FIGURE 5 Phylogenetic relationships of five Semisulcospira species estimated by the Neighbor-Net reconstructed based on uncorrected p-distances of 738 SNPs	FIGURE 5 Phylogenetic relationships of five Semisulcospira species estimated by the Neighbor-Net reconstructed based on uncorrected p-distances of 738 SNPs	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF7AD5C38DFF12AEED1C821.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349640/files/figure.png	https://doi.org/10.5281/zenodo.8349640	FIGURE 10 Shells of Semisulcospira nakanoi sp. nov. A–G,Holotype, KUZ Z4122.H–J, Paratype, KUZ Z4125. K–L, Paratype, KUZ Z4126.M–Q, Specimens from Onoe Port, KUZ Z4129, Z4130.A–C, H, K, M, P, Adult shell. A–C, H, M, Female.K, P,Male.D, I, L, N, Q, Operculum.E–G, J, O, Embryonic shell.Scale bars: 10 mm (A–D, H–I, K–L, M–N, P–Q), 1 mm (E–G, J, O). All specimens were treated with 3% sodium hypochlorite	FIGURE 10 Shells of Semisulcospira nakanoi sp. nov. A–G,Holotype, KUZ Z4122.H–J, Paratype, KUZ Z4125. K–L, Paratype, KUZ Z4126.M–Q, Specimens from Onoe Port, KUZ Z4129, Z4130.A–C, H, K, M, P, Adult shell. A–C, H, M, Female.K, P,Male.D, I, L, N, Q, Operculum.E–G, J, O, Embryonic shell.Scale bars: 10 mm (A–D, H–I, K–L, M–N, P–Q), 1 mm (E–G, J, O). All specimens were treated with 3% sodium hypochlorite	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF7AD5C38DFF12AEED1C821.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349644/files/figure.png	https://doi.org/10.5281/zenodo.8349644	FIGURE 12 Radulae of Semisulcospira species.A–G, Semisulcospira niponica: A, Oura Port, KUZ Z4091; B, Nagahama Port, KUZ Z4093; C, Iso, KUZ Z4095; D, Kitakomatsu Port, KUZ Z4097; E, Katata Port, KUZ Z4099; F, Otsu Port, KUZ Z4101; G, Nango, KUZ Z4103.H–J, Semisulcospira fuscata: H, Lake Yogo, KUZ Z4103; I, Oura, KUZ Z4105; J, Nihonmatsu, KUZ Z4107.K–L, Semisulcospira watanabei sp. nov.:K, Kitakomatsu Port, KUZ Z4115;L, Horikiri Port, KUZ Z4120.M–N, Semisulcospira nakanoi sp. nov.: M, Onoe Port, KUZ Z4129;N, Chikubu-shima Island, KUZ Z4127.O–P, Semisulcospira salebrosa sp. nov.:O, Take-shima Island, KUZ Z4138;P, Shiraishi-jima Island, KUZ Z4136.Scale bars:100 Μm	FIGURE 12 Radulae of Semisulcospira species.A–G, Semisulcospira niponica: A, Oura Port, KUZ Z4091; B, Nagahama Port, KUZ Z4093; C, Iso, KUZ Z4095; D, Kitakomatsu Port, KUZ Z4097; E, Katata Port, KUZ Z4099; F, Otsu Port, KUZ Z4101; G, Nango, KUZ Z4103.H–J, Semisulcospira fuscata: H, Lake Yogo, KUZ Z4103; I, Oura, KUZ Z4105; J, Nihonmatsu, KUZ Z4107.K–L, Semisulcospira watanabei sp. nov.:K, Kitakomatsu Port, KUZ Z4115;L, Horikiri Port, KUZ Z4120.M–N, Semisulcospira nakanoi sp. nov.: M, Onoe Port, KUZ Z4129;N, Chikubu-shima Island, KUZ Z4127.O–P, Semisulcospira salebrosa sp. nov.:O, Take-shima Island, KUZ Z4138;P, Shiraishi-jima Island, KUZ Z4136.Scale bars:100 Μm	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF7AD5C38DFF12AEED1C821.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349620/files/figure.png	https://doi.org/10.5281/zenodo.8349620	FIGURE 3 Results of principal components analysis based on 738 SNPs conducted for five Semisulcospira species. A, Principal component (PC) 1 vs PC2.B, PC2 vs PC3. C, PC3 vs PC4. D, PC4 vs PC5	FIGURE 3 Results of principal components analysis based on 738 SNPs conducted for five Semisulcospira species. A, Principal component (PC) 1 vs PC2.B, PC2 vs PC3. C, PC3 vs PC4. D, PC4 vs PC5	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF7AD5C38DFF12AEED1C821.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349624/files/figure.png	https://doi.org/10.5281/zenodo.8349624	FIGURE 4 Results of ADMIXTURE analysis based on 738 SNPs conducted for five Semisulcospira species.Bar colours of the species in K = 5 correspond to the colour coding in fig.3	FIGURE 4 Results of ADMIXTURE analysis based on 738 SNPs conducted for five Semisulcospira species.Bar colours of the species in K = 5 correspond to the colour coding in fig.3	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF7AD5C38DFF12AEED1C821.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349630/files/figure.png	https://doi.org/10.5281/zenodo.8349630	FIGURE 6 Map of Lake Biwa indicating geographical variation in the frequency of sculpture types in the adult females of five Semisulcospira species.Colours of locality names correspond to the colour coding in fig.1	FIGURE 6 Map of Lake Biwa indicating geographical variation in the frequency of sculpture types in the adult females of five Semisulcospira species.Colours of locality names correspond to the colour coding in fig.1	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF7AD5C38DFF12AEED1C821.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349626/files/figure.png	https://doi.org/10.5281/zenodo.8349626	FIGURE 5 Phylogenetic relationships of five Semisulcospira species estimated by the Neighbor-Net reconstructed based on uncorrected p-distances of 738 SNPs	FIGURE 5 Phylogenetic relationships of five Semisulcospira species estimated by the Neighbor-Net reconstructed based on uncorrected p-distances of 738 SNPs	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF7AD5C38DFF12AEED1C821.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349616/files/figure.png	https://doi.org/10.5281/zenodo.8349616	FIGURE 1 Map of Lake Biwa (A) and Oura Bay (B) showing 15 sampling localities:blue, Semisulcospira niponica; green, S.fuscata; purple, S.watanabei sp.nov.; red, S.nakanoi sp.nov.; orange, S.salebrosa sp.nov; black, putative hybrid between S.fuscata and S.watanabei sp.nov.	FIGURE 1 Map of Lake Biwa (A) and Oura Bay (B) showing 15 sampling localities:blue, Semisulcospira niponica; green, S.fuscata; purple, S.watanabei sp.nov.; red, S.nakanoi sp.nov.; orange, S.salebrosa sp.nov; black, putative hybrid between S.fuscata and S.watanabei sp.nov.	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF1AD5E38DFF041EF5ACF87.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349642/files/figure.png	https://doi.org/10.5281/zenodo.8349642	FIGURE 11 Shells of Semisulcospira salebrosa sp.nov.A–G, Holotype, KUZ Z4131.H–J, Paratype, KUZ Z4133.K–L, Paratype, KUZ Z4135.M–O, Specimen from Take-shima Island, KUZ Z4138.A–C, H, K, M, Adult shell. A–C, H, M, Female.K, Male. D, I, L, N, Operculum.E–G, J, O, Embryonic shell. Scale bars:10 mm (A–D, H–I, K–L,M–N), 1 mm (E–G, J, O). All specimens were treated with 3% sodium hypochlorite	FIGURE 11 Shells of Semisulcospira salebrosa sp.nov.A–G, Holotype, KUZ Z4131.H–J, Paratype, KUZ Z4133.K–L, Paratype, KUZ Z4135.M–O, Specimen from Take-shima Island, KUZ Z4138.A–C, H, K, M, Adult shell. A–C, H, M, Female.K, Male. D, I, L, N, Operculum.E–G, J, O, Embryonic shell. Scale bars:10 mm (A–D, H–I, K–L,M–N), 1 mm (E–G, J, O). All specimens were treated with 3% sodium hypochlorite	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF1AD5E38DFF041EF5ACF87.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349644/files/figure.png	https://doi.org/10.5281/zenodo.8349644	FIGURE 12 Radulae of Semisulcospira species.A–G, Semisulcospira niponica: A, Oura Port, KUZ Z4091; B, Nagahama Port, KUZ Z4093; C, Iso, KUZ Z4095; D, Kitakomatsu Port, KUZ Z4097; E, Katata Port, KUZ Z4099; F, Otsu Port, KUZ Z4101; G, Nango, KUZ Z4103.H–J, Semisulcospira fuscata: H, Lake Yogo, KUZ Z4103; I, Oura, KUZ Z4105; J, Nihonmatsu, KUZ Z4107.K–L, Semisulcospira watanabei sp. nov.:K, Kitakomatsu Port, KUZ Z4115;L, Horikiri Port, KUZ Z4120.M–N, Semisulcospira nakanoi sp. nov.: M, Onoe Port, KUZ Z4129;N, Chikubu-shima Island, KUZ Z4127.O–P, Semisulcospira salebrosa sp. nov.:O, Take-shima Island, KUZ Z4138;P, Shiraishi-jima Island, KUZ Z4136.Scale bars:100 Μm	FIGURE 12 Radulae of Semisulcospira species.A–G, Semisulcospira niponica: A, Oura Port, KUZ Z4091; B, Nagahama Port, KUZ Z4093; C, Iso, KUZ Z4095; D, Kitakomatsu Port, KUZ Z4097; E, Katata Port, KUZ Z4099; F, Otsu Port, KUZ Z4101; G, Nango, KUZ Z4103.H–J, Semisulcospira fuscata: H, Lake Yogo, KUZ Z4103; I, Oura, KUZ Z4105; J, Nihonmatsu, KUZ Z4107.K–L, Semisulcospira watanabei sp. nov.:K, Kitakomatsu Port, KUZ Z4115;L, Horikiri Port, KUZ Z4120.M–N, Semisulcospira nakanoi sp. nov.: M, Onoe Port, KUZ Z4129;N, Chikubu-shima Island, KUZ Z4127.O–P, Semisulcospira salebrosa sp. nov.:O, Take-shima Island, KUZ Z4138;P, Shiraishi-jima Island, KUZ Z4136.Scale bars:100 Μm	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF1AD5E38DFF041EF5ACF87.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349620/files/figure.png	https://doi.org/10.5281/zenodo.8349620	FIGURE 3 Results of principal components analysis based on 738 SNPs conducted for five Semisulcospira species. A, Principal component (PC) 1 vs PC2.B, PC2 vs PC3. C, PC3 vs PC4. D, PC4 vs PC5	FIGURE 3 Results of principal components analysis based on 738 SNPs conducted for five Semisulcospira species. A, Principal component (PC) 1 vs PC2.B, PC2 vs PC3. C, PC3 vs PC4. D, PC4 vs PC5	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF1AD5E38DFF041EF5ACF87.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349616/files/figure.png	https://doi.org/10.5281/zenodo.8349616	FIGURE 1 Map of Lake Biwa (A) and Oura Bay (B) showing 15 sampling localities:blue, Semisulcospira niponica; green, S.fuscata; purple, S.watanabei sp.nov.; red, S.nakanoi sp.nov.; orange, S.salebrosa sp.nov; black, putative hybrid between S.fuscata and S.watanabei sp.nov.	FIGURE 1 Map of Lake Biwa (A) and Oura Bay (B) showing 15 sampling localities:blue, Semisulcospira niponica; green, S.fuscata; purple, S.watanabei sp.nov.; red, S.nakanoi sp.nov.; orange, S.salebrosa sp.nov; black, putative hybrid between S.fuscata and S.watanabei sp.nov.	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
BE0D87E1FFF1AD5E38DFF041EF5ACF87.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/8349626/files/figure.png	https://doi.org/10.5281/zenodo.8349626	FIGURE 5 Phylogenetic relationships of five Semisulcospira species estimated by the Neighbor-Net reconstructed based on uncorrected p-distances of 738 SNPs	FIGURE 5 Phylogenetic relationships of five Semisulcospira species estimated by the Neighbor-Net reconstructed based on uncorrected p-distances of 738 SNPs	2022-08-22	Sawada, Naoto;Fuke, Yusuke		Zenodo	biologists	Sawada, Naoto;Fuke, Yusuke			
