taxonID	type	description	language	source
8300DF3D4231A22CFF2BFF455A5E62DE.taxon	etymology	Etymology: — Referring to the host genus Juglans on which the fungus was collected.	en	Wang, Fei-Hu, Wang, Yan-Lin, Zeng, Qian, Xu, Xiu-Lan, Liu, Ying-Gao, Yang, Chun-Lin (2023): A novel species of Loculosulcatispora (Sulcatisporaceae, Pleosporales) on Juglans regia from Sichuan, China. Phytotaxa 620 (4): 264-272, DOI: 10.11646/phytotaxa.620.4.2, URL: http://dx.doi.org/10.11646/phytotaxa.620.4.2
8300DF3D4231A22CFF2BFF455A5E62DE.taxon	materials_examined	Type: — China, Sichuan Province, Neijiang City, 29 ° 48 ′ 30 ′′ N, 105 ° 6 ′ 36 ′′ E, 340 m, on dead twigs of Juglans regia (Juglandaceae), 17 May 2021, F. H. Wang, WFH 202105006, (SICAU 22 - 0078, holotype), ex-type culture SICAUCC 22 - 0079. Saprobic on dead twigs of Juglans regia. Sexual morph: Ascomata 370 – 470 × 410 – 520 µm (x = 380 × 430 µm, n = 10), solitary, scattered, or sometimes gregarious, immersed, erumpent at maturity, dome-shaped to ellipsoid or irregular, black, uni-loculate, glabrous, with a central ostiole. Ostioles 60 – 90 × 80 – 140 μm (x = 75 × 110 μm, n = 40), single, black, short papillate. Peridium 10 – 13 μm, thick, unequal thickness, outer layer comprising brown to black cells of textura angularis, internal wall thin and hyaline. Pseudoparaphyses 1 – 2.5 μm wide, dense, filamentous, septate, unbranched, hyaline, arising around inner wall of locules. Asci (46 –) 78 – 112 (– 135) × (7 –) 9 – 21 μm (x = 90 × 14 μm, n = 40), 4 - spored, bitunicate, fissitunicate, clavate, pedicellate, apically rounded with well-developed ocular chamber. Ascospores (20 –) 26 – 36 (– 34) × (8 –) 9 – 12 (– 14) μm (x = 31 × 10 μm, n = 50), overlapping, uni- to bi-seriate, hyaline, fusiform, broad fusiform with oval ends, 1 - septate, distinctly constricted at septum, smooth-walled, with two large droplets in each cell, surrounded by inapparent, thin, sheath at ends. Asexual morph: Undetermined. Culture characteristics: — Ascospores germinated in sterilized water within 24 h and producing germ tubes from both ends. The colonies on PDA exhibited slow growth, achieving a diameter of approximately 2.8 cm after 25 days at 25 ºC under a 12 - hour light / 12 - hour dark cycle, featuring circular, white-grey centers with white margins, floccose texture, slight elevation, smooth edges, white granular surface spots, circular cracks, and a red-brown ring on the reverse side. Notes: — Loculosulcatispora juglans forms a sister clade to L. hongheensis, L. thailandica, and Parasulcatispora clematidis with high statistical support (FIG. 1). Morphologically, L. juglans differs from the sexual descriptions of L. hongheensis and P. clematidis. A morphometric analysis revealed that the ascomata, asci, and ascospores of P. clematidis were comparatively smaller than those of L. hongheensis and L. juglans. Notably, P. clematidis (MFLUCC 17 - 2082) exhibited the unique trait of oil droplet production in culture, a characteristic not observed in L. hongheensis and L. juglans. A morphological comparison of L. juglans, L. hongheensis, and P. clematidis is presented in TABLE 2. Nucleotide comparisons of ITS, LSU, SSU, tef 1 - α, and rpb 2 sequences from our isolate showed discrepancies with sequences from L. hongheensis (HKAS 122920, holotype), with similarities of 91 % (697 / 762, 23 gaps), 98.98 % (877 / 886, 3 gaps), 99.3 % (996 / 1003, 1 gap), 96.8 % (877 / 906, 36 gaps), and 89.5 % (936 / 1046, 39 gaps), respectively. Comparisons with L. thailandica (MFLUCC 20 - 0108, holotype) revealed similarities of 97.4 % (742 / 762, 12 gaps), 96.3 % (853 / 886, 2 gaps), 88.4 % (887 / 1003, 1 gaps), 99.9 % (877 / 878, 38 gaps), and 71.9 % (752 / 1046, 22 gaps), respectively. Furthermore, comparisons with P. clematidis (MFLUCC 17 - 2082, holotype) indicated similarities of ITS 66.9 % (510 / 762, 53 gap), LSU 97.5 % (864 / 886, 5 gaps) and tef 1 - α 98.3 % (877 / 892, 39 gaps), respectively. Therefore, considering the observed morphological distinctions and strong phylogenetic support, we hereby propose the introduction of L. juglans sp. nov.	en	Wang, Fei-Hu, Wang, Yan-Lin, Zeng, Qian, Xu, Xiu-Lan, Liu, Ying-Gao, Yang, Chun-Lin (2023): A novel species of Loculosulcatispora (Sulcatisporaceae, Pleosporales) on Juglans regia from Sichuan, China. Phytotaxa 620 (4): 264-272, DOI: 10.11646/phytotaxa.620.4.2, URL: http://dx.doi.org/10.11646/phytotaxa.620.4.2
