identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
DE6387EA430D996140C5F8BE73B6835A.text	DE6387EA430D996140C5F8BE73B6835A.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amanita sharmae A. Kumar, Mehmood, Verma K & A. Ghosh 2023	<html xmlns:mods="http://www.loc.gov/mods/v3">
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            <p> Amanita sharmae A. Kumar, Mehmood, Verma K &amp; A. Ghosh sp. nov. Figs. 2, 3 </p>
            <p>MycoBank: MB 844382</p>
            <p> GenBank: ON679656 (nrITS, holotype), OP382962 (nrITS); ON679647 (nrLSU, holotype), OP391537 (nrLSU) . </p>
            <p> Diagnosis:—Distinct from all known species of  Amanita sect. Vaginatae by its dark grey to olive grey pileus at center, olive brown towards margin, inamyloid broadly ellipsoid to ellipsoid basidiospores measuring (10‒14 × 7‒10 μm), presence of clamp connections, its occurrence under coniferous forest dominated by  Abies pindrow and  Picea smithiana and 2- gene phylogenetic inference. </p>
            <p> Etymology:—‘  sharmae ’ refers to Prof. Yash Pal Sharma to honour his valuable contributions to the macrofungal flora of Jammu and Kashmir and Ladakh. </p>
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                  Typification:— INDIA. Jammu and Kashmir: Kishtwar district,  
                <a title="Search Plazi for locations around (long 75.76945/lat 33.31278)" href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=75.76945&amp;materialsCitation.latitude=33.31278">Chatroo</a>
                 , 33°18′46″N 75°46′10″E, Elev. 2068 m, 26 th August 2021, A  .  Kumar, AKA-13 (CAL 1882, holotype!) . 
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            <p>Description: —Basidiomata small to medium-sized. Pileus 30–70 mm diam., initially convex, becoming convex to plano-convex, sometimes umbonate or slightly depressed at center, greyish brown (7E3) to dark brown (6F5) at center, dark grey (1F1) to olive grey (1F1–2), olive brown (4E3–4) towards margin, slightly viscid when moist, smooth, covered with greyish brown (7E3) to olive grey (1F1–2) minute fibrils, mostly lacking universal veil remnants; margin short-striate up to 10–15 mm, non-appendiculate, initially incurved, uplifted at maturity; trama white (1A1), 2–4 mm thick, thinning evenly toward margin, pale cream, not bruising or staining. Lamellae free, crowded, white (1A1); lamellar edges white (1A1); lamellulae truncate, in 4–5 tiers. Stipe 70–110 mm × 7–8 mm (excluding bulb), slightly tapering upward, chalky white (1A1) covered with minute white fibrils, context white (1A1), hollow in center; basal bulb absent; volva saccate, 30–40 mm high × 15–20 mm wide, membranous, outer surface white (1A1) to greyish white (1BI), interior white (1A1). Annulus absent. Odor and taste not observed.</p>
            <p>Lamellar trama bilateral. Mediostratum 40–60 μm wide, composed of abundant, broadly ellipsoid to elongated, inflated cells (40–90 × 18–50 μm); filamentous undifferentiated hyphae abundant, 6–10 μm wide, thin-walled colorless, hyaline. clamp connections present. Lateral stratum composed of ellipsoid to cylindrical inflated cells (25‒45 × 10‒15 μm) with abundant interwoven filamentous hyphae which are 3‒6 μm in diam., clamp connections present. Subhymenium 30–55 μm thick, with 2‒4 layers, composed of subglobose to ovoid to ellipsoid cells 10–45 × 8–25 μm. Basidia 50‒73 × 10‒15 μm, 2‒4 spored, clavate, 4-spored, thin-walled; sterigmata 2–4 μm long, basal septa often clamped. Basidiospores [60/3/3] (9.0‒)10.0‒14.0(‒16) × (6.5‒)7.0‒10.0(‒11.0) μm, Q = (1.10‒)1.2‒1.57(‒1.66), Qm = 1.37, broadly ellipsoid to ellipsoid, inamyloid, colorless, thin-walled, smooth; apiculus 0.7 μm. Lamellar edge sterile, composed of ellipsoid cells (15–40 × 10‒30 μm), filamentous hyphae abundant, 3‒5 μm in diam., irregularly arranged, hyaline. Pileipellis 50–160 μm thick, two-layered; upper layer (30–65 μm) thick, gelatinized, composed of radially to parallelly arranged, thin-walled, colorless, filamentous undifferentiated hyphae 2–6 μm wide; lower layer (20–95 μm) thick composed of radially arranged, filamentous hyphae 5–14 μm wide, clamp connections present. Outer surface of volval remnants on stipe base composed of longitudinally arranged to interwoven elements; filamentous undifferentiated hyphae very abundant to dominant 2–8 μm wide, colorless, thin-walled 6–8 μm wide, branching, inflated cells scarce to scattered, subglobose to ellipsoid cells (60–90 × 45–60 μm), colorless, thin-walled, vascular hyphae scarce, clamp connections present. Interior of volval remnants on stipe base composed of longitudinally arranged to interwoven elements: filamentous hyphae very abundant to dominant, 2–8 μm wide, colorless, thin-walled, 6–8 μm wide, branching; inflated cells scarce to scattered, subglobose to ellipsoid cells (60–90 × 45–60 μm), colorless, thin-walled, vascular hyphae scarce, clamp connections present. Stipe trama composed of longitudinally arranged filamentous hyphae, 3‒7 μm wide, hyaline, clavate terminal cells 90‒290 × 15‒25 μm, clamp connections present.</p>
            <p> Habitat &amp; distribution:—Solitary to sub-gregarious in temperate coniferous forest dominated by  Abies pindrow and  Picea smithiana . </p>
            <p>Known distribution:—Only collected from northwestern Himalaya of Jammu Province, India.</p>
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                 Additional specimens examined:—   INDIA. Jammu and Kashmir: Kathua District,  
                <a title="Search Plazi for locations around (long 75.72892/lat 32.825752)" href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=75.72892&amp;materialsCitation.latitude=32.825752">Sarthal</a>
                 , 32°49’32.71”N, 75°43’44.12”E, Elev. 3059 m, 25 th September 2019, A. Kumar &amp; K. Verma, AKS-22; Udhampur, Latti, Elev. 1973 m., 5 th September 2021, A. Kumar &amp; K. Verma, AKS-37  . 
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	https://treatment.plazi.org/id/DE6387EA430D996140C5F8BE73B6835A	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Kumar, Anil;Verma, Komal;Ghosh, Aniket;Mehmood, Tahir	Kumar, Anil, Verma, Komal, Ghosh, Aniket, Mehmood, Tahir (2023): Morphological and molecular data reveal a new species of Amanita section Vaginatae (Amanitaceae) from India. Phytotaxa 584 (3): 186-196, DOI: 10.11646/phytotaxa.584.3.5, URL: http://dx.doi.org/10.11646/phytotaxa.584.3.5
