taxonID	type	description	language	source
038587B2FFB6FFB6FF2DFCA9EFA1FF05.taxon	description	Figs. 1 - 2	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB6FFB6FF2DFCA9EFA1FF05.taxon	materials_examined	Material examined — Holotype ♂ (RMNH), Iran, Golestan Province, IR 3 Shirabad near Khanbebin, stream, downstream near bridge, 36.967499 N, 55.027779 E, 27. viii. 2017 leg. Pešić, Zawal & Saboori, dissected and slidemounted. Paratypes: 2 ♂, 1 ♀, same place and data as holotype (RMNH); 1 ♂ sequenced [CCDB 38361 F 03]; 1 ♀ dissected and slide-mounted (RMNH).	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB6FFB6FF2DFCA9EFA1FF05.taxon	diagnosis	Diagnosis — Integument papillae rounded. Genital plates with 40 – 51 pairs of Ac in at most four longitudinal rows. Numbers of leg swimming setae: II-L-posterior 1; III-L- 4 posterior 6 - 7; III-L- 5 posterior 5; IV-L- 4 anterior 6 - 8, posterior 6 - 7; IV-L- 5 anterior 2 - 3, posterior 4 - 5.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB6FFB6FF2DFCA9EFA1FF05.taxon	description	Description. Both sexes: Integument papillae rounded (Fig. 1 C). Cx-I + II medially separated by a fine membranous line, with a row of long fine setae at medial margins of Cx-I, and posterior margins of Cx-I, Cx-II, - III, and – IV. Genital flaps with rounded lateral and concave medial margins (Figs. 1 A-B). Excretory pore sclerotized. Leg claws with a dorsal clawlet. Male (holotype; in parentheses some measurements of the paratype specimen [CCDB 38361 F 03]): Idiosoma L 1130, W 1090. Coxal field L 527; Cx-III W 559, L Cx-I + II 266, Cx-III + IV 278; coxal setae numbers: Cx-I, 24 (23); Cx-II, 23 (22); Cx-III, 14 (18); Cx-IV 11 (19). Genital plate (Fig. 1 A) L 259 (238), on each plate Ac number 49 - 51 (49), with 36 - 40 setae, all hollow. Ejaculatory complex L 198. Gnathosoma vL 228; chelicera (Fig. 2 D) total L 297, L basal segment 238, claw 63, L ratio basal segment / claw 3.8. Palp (Figs. 2 A-B) total L 444, dL / H, dL / H ratio: P- 1, 55 / 45, 1.23; P- 2, 73 / 56, 1.3; P- 3, 50 / 53, 0.94; P- 4, 186 / 47, 4.0; P- 5, 80 / 22, 3.6; L ratio P- 2 / P- 4, 0.4. dL IV-L: 128, 150, 200, 309, 313, 291. Female (paratype, n = 1; juvenile specimen ([CCDB 38361 F 05]): Idiosoma L 790. Coxal field L 486; Cx-III W 494, L Cx-I + II 256, Cx-III + IV 281; coxal setae numbers: Cx-I, 18; Cx-II, 18; Cx-III, 13; Cx-IV 12. Genital plate large, L 210 - 217, on each plate Ac number 40 - 42, with 30 setae. Gnathosoma vL 228; chelicera total L 297, L basal segment 233, claw 63, L ratio basal segment / claw 3.7. Palp total L 446, dL / H, dL / H ratio: P- 1, 53 / 45, 1.19; P- 2, 77 / 57, 1.34; P- 3, 52 / 55, 0.94; P- 4, 189 / 42, 4.5; P- 5, 75 / 22, 3.4; L ratio P- 2 / P- 4, 0.41. dL IV-L: 125, 150, 205, 309, 328, 272. Species delimitation using DNA-barcodes — The final alignment for species delimitation using COI sequence data of ten Hydrodroma specimens listed in Table 2 and one outgroup, Protzia cabardinica (BOLD: 8. IR 8 2017 H 2) from Iran to root the tree. The NJ tree is presented in Fig. 3. The COI tree sequence retrieved from a specimen of H. golestanica sp. nov. from Iran appeared as a sister taxa of H. torrenticola (Fig. 3). The average K 2 P genetic distance between the COI sequence of one specimen of H. golestanica sp. nov. from Iran and a specimen of H. torrenticola of from Montenegro was estimated to 10.1 % (Table 3).	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB6FFB6FF2DFCA9EFA1FF05.taxon	etymology	Etymology — Named after the province where the new species was collected.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB6FFB6FF2DFCA9EFA1FF05.taxon	discussion	Discussion — The new species from Golestan Province closely resembles Hydrodroma torrenticola, a species widely distributed in the Palaearctic (Di Sabatino et al. 2010). It shares the presence of only one short swimming seta on II-L- 5 and 2 - 3 swimming setae on anterior IV-L- 5. Only one specimen of the new species could be acquired for use in molecular analyses, so intraspecific differences in COI sequence could not be examined. The phylogenetic analysis based on COI data reveals that the new species is most similar to H. torrenticola. Level of COI differentiation between the new species from Iran and H. torrenticola from Montenegro was 10.1 % K 2 P divergence. From a morphological point of view, H. torrenticola can be separated from the new species by the shape of the integument papillae (pointed vs. distally rounded in H. golestanica sp. nov.).	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB6FFB6FF2DFCA9EFA1FF05.taxon	distribution	Distribution — Iran.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB2FFB5FF2DFED8EF94FB81.taxon	materials_examined	Material examined — Iran, Mazandaran Province, IR 7 small stream along road to Kandelous village, 36.426388 N, 51.470001 E, 28. viii. 2017 leg. Pešić, Zawal & Saboori, 3 ♀ (sequenced [CCDB 38233 F 09, - F 11, - G 01]).	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB2FFB5FF2DFED8EF94FB81.taxon	discussion	Remarks — Sperchon amuzgari was described by Bader & Sepasgosarian (1979) from a karstic spring in Lorestan Province, Iran. Later on, Asadi et al. (2010) re-examined the holotype of S. amuzgari and found that in view of the similar shape of the palp and dorsum, III / IV-L- 3 – 5 with numerous pinnate dorsal setae and sclerotized excretory pore the later species matches the morphology of S. hispidus Koenike, 1895, a species widely distributed in the Western Palaearctic (Di Sabatino et al. 2010). As a result, Asadi et al. (2010) proposed to place S. amuzgari in synonymy with S. hispidus. During our 2017 survey in Northern Iran we collected and successfully barcoded three specimens of hispidus - like mites that match the original description of S. amuzgari. According to the original description, S. amuzgari can be separate from S. hispidus by having a parallelogram-shaped Cx-IV (Bader & Sepasgosarian 1979). The final alignment for species delimitation using COI sequence data comprised sequences of 34 Sperchon specimens listed in Table 4 and one outgroup, Sperchonopsis verrucosa (Protz, 1896) [BOLD 46. M 19 _ 16 B _ 1 _ G 10] from Montenegro to root the tree. The neighbor-joining (NJ) tree is presented in Fig. 4. The COI tree sequence recovered S. amuzgari as a sister branch to the clade grouping COI sequences found in S. hispidus (Fig. 4). The average genetic distance between the COI sequence of the three specimens of S. amuzgari from Iran and two specimens of S. hispidus from Montenegro was estimated to 16.5 % K 2 P indicating a long independent history of these two species. Therefore, we propose to resurrect Sperchon amuzgari Bader & Sepasgosarian, 1979 as a valid species distinct from S. hispidus. Distribution — Iran.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB3FFBBFF2DF953ECF3FCC5.taxon	materials_examined	Material examined — Sperchon papillosus Thor, 1901. Iran, Mazandaran Province, IR 7 small stream along road to Kandelous village, 36.426388 N, 51.470001 E, 28. viii. 2017 leg. Pešić, Zawal & Saboori, 2 ♂, 1 ♀ (2 ♂ sequenced [DCCDB 072 - 21, IRANM 001 - 20]). Sperchon compactilis Koenike, 1911. Golestan Province, IR 2 Kaboud-val waterfall, 36.869720 N, 54.889168 E, 26. viii. 2017 leg. Pešić, Zawal & Saboori, 1 ♂ (sequenced [IRANM 002 - 20], dissected and slide mounted [5. IR 2 2017 G 12]).	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFB3FFBBFF2DF953ECF3FCC5.taxon	discussion	Remarks — Asadi et al. (2010) re-examined the holotype of Sperchon bispinosus Bader & Sepasgosarian, 1982 a species described by Bader & Sepasgosarian (1982) from Elburs Mountain, North Iran and placed this species into synonymy of S. papillosus Thor, 1901. Sperchon papillosus matches the original description of S. bispinosus in having integument sculpture made up of a network of fine denticles combined with additional larger papillae and the similar shape of dorsum and palp (Asadi et al. 2010). During our 2017 survey we collected and successfully barcoded three specimens from the S. papillosus / S. compactilis - complex from North Iran. Two specimens from a small stream along the road to Kandelous village match the description of S. bispinosus / S. papillosus: integument sculpture made up of a network of fine denticles combined with additional larger papillae and P- 3 with two ventral setae. One specimen from Kaboud-val waterfall lacks the ventral setae on P- 3 and we assigned it to S. compactilis. In our COI tree (Fig. 4) the sequence of the S. compactilis specimen from Iran represents a unique BIN (BOLD: AEI 4363) and falls within a clade alongside the sequences from S. bispinosus specimens from Iran, which were grouped in a separate BIN (BOLD: AED 2135). The examined specimens from Iran form a clade that is sister to the clade containing S. compactilis specimens (BIN BOLD: AEI 4363) from the Netherlands, all nested within a clade that also includes a specimen of Sperchon papillosus from Montenegro that represent a unique BIN (BOLD: AED 2134). The genetic distance between COI sequence groups (BINs) of examined specimens of S. papillosus / S. compactilis complex ranged from 1.79 % K 2 P between Iranian specimens of S. papillosus and S. compactilis, to 5.11 % K 2 P between S. papillosus specimen from Montenegro and specimens of S. compactilis from the Netherlands (see Table 5). Further studies integrating more markers and additional sampling is necessary to clarify the taxonomy of species of the S. papillosus / S. compactilis complex.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFBDFFB9FF2DF9E2EFA1FEBD.taxon	materials_examined	Material examined — Iran, Mazandaran Province, IR 13 area of Ramsar city, Elburs Mountain, small waterfall, 36.8897 N, 50.5681 E, 29. viii. 2017 leg. Pešić, Zawal & Saboori, 2 ♀ (sequenced [CCDB 38233 F 07, - F 08]).	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFBDFFB9FF2DF9E2EFA1FEBD.taxon	discussion	Remarks — Monatractides persicus Pešić, 2004 was described from a stream in Elburs mountain of North Iran (Pešić & Saboori 2004). Later on, this species was placed in synonymy with M. stadleri (Walter, 1924) by Asadi et al. (2010). The latter authors felt that some differences such as the presence of knob-shaped protrusions at the margin of the gnathosomal bay are not suitable for separating the two species. The specimen from Northern Iran used in this study for molecular analysis perfectly matches the description of M. persicus. The latter species can be distinguished from M. stadleri on the basis of a relatively thicker chelicera (ratio L / H 5.6 – 5.9 vs. 6.2 – 6.4, data taken from Pešić & Saboori 2004). Based on our COI tree, Monatractides persicus is a sister taxon of M. stadleri (see Fig. 6). The average K 2 P genetic distance between M. persicus and M. stadleri was estimated to 9.35 %. Thus, the morphological and molecular data suggest that M. persicus is a valid species distinct from M. stadleri.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFBDFFB9FF2DF9E2EFA1FEBD.taxon	distribution	Distribution — Iran.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFBEFFB8FF2DF88CEFA1F9AC.taxon	materials_examined	Material examined — Iran, Golestan Province, Shirabad near Khanbebin, stream, downstream near bridge, gravel, 36.967499 N, 55.027779 E, 27. viii. 2017 leg. Pešić, Zawal & Saboori, 3 ♂ (1 ♂ sequenced [DCDDJ 067 - 21], dissected and slide mounted [CCDB 38361 F 07]).	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFBEFFB8FF2DF88CEFA1F9AC.taxon	discussion	Remarks — Torrenticola baueri was described by Bader & Sepasgozarian (1987) from a stream in Elburs mountain of North Iran. The authors compared T. baueri with the morphologically distant T. anomala, but were not aware of the close similarity to T. barsica. Laster on, Asadi et al. (2010) re-examined the holotype of T. baueri and found that this species matches the morphology and measurement ranges of Torrenticola barsica (Szalay, 1933), a species widely distributed in the Western Palaearctic. The only difference was found in a slightly shorter medial suture Cx-II + III in male (97 µm in T. baueri vs. 105 - 110 µm in T. barsica). As a result the latter authors synonymized T. baueri with T. barsica. During our 2017 survey in Northern Iran we collected several specimens of barsica - like mites which morphologically match the original description of T. baueri and measurements given by Asadi et al. (2010). One specimen [CCDB 38361 F 07] was successfully barcoded and used for species delimitation. For the final alignment of species delimitation using COI sequence data we used the 34 Torrenticola specimens listed in Table 7 and one outgroup, Monatractides persicus Pešić, 2004 to root the tree. The neighbor-joining (NJ) tree is presented in Fig. 7. The COI sequence retrieved from the Torrenticola baueri specimen collected in Iran was recovered as sister to T. barsica (Fig. 7). The average K 2 P genetic distance between the COI sequence of the specimen of T. baueri from Iran and two specimens of T. barsica from Montenegro was 17.4 % suggesting a long independent history of these two species. Therefore, we resurrected T. baueri as a valid species distinct from T. barsica.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
038587B2FFBEFFB8FF2DF88CEFA1F9AC.taxon	distribution	Distribution — Iran.	en	Pešić, Vladimir, Zawal, Andrzej, Saboori, Alireza, Smit, Harry (2021): New records of water mites (Acari, Hydrachnidia) from Iran with the description of one new species based on morphology and DNA barcodes. Zootaxa 5082 (5): 425-440, DOI: https://doi.org/10.11646/zootaxa.5082.5.2
