identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03F1879F521C8D03FF040B9DFE53312B.text	03F1879F521C8D03FF040B9DFE53312B.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Microseira minor Geng et Yu 2021	<html xmlns:mods="http://www.loc.gov/mods/v3">
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            <p> Microseira minor Geng et Yu sp. nov. (Fig. 1) </p>
            <p> Diagnosis: —This species is morphologically similar to the genus  Microseira but differs from  M. wollei in the lack of false branching, much narrower filaments and trichomes, smaller ratio of cell length and width under the light microscopy. The phylogenetic analysis based on 16S rRNA gene, partial 16S-23S rRNA operon and nifH gene showed that this species was formed a well-supported clade, closest to other  Microseira species , far away from other close genera. In addition, the non-producing of neurotoxins and hepatotoxins is also a major difference. </p>
            <p>Description: —Filaments unbranched, long, 13.73–(16.04)–18.58 μm broad, forming densely aggregations, blueblack, brown-black, yellowed when old, straight or slightly flexuous. Trichomes cylindrical, isopolar, not attenuated to the ends, not or slightly constricted at the cross-walls. Cells discoid, 3.12–(5.03)–7.70 μm long, 9.02–(11.05)–13.33 μm broad, 2–3 × broader than long, with granular content, not aerotopes. Apical cells rounded, without calyptra or thickened outer cell wall. Sheath usually present, colorless, hyaline, firm, lamellated when old, and open at the apex. Reproduction by motile hormogonia formed by help of necridia. Thylakoids irregularly arranged.</p>
            <p> Etymology: —The name of species “  minor ” was chosen because of its small and thin discoid cells. </p>
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                 Type locality: —   
                <a title="Search Plazi for locations around (long 116.11061/lat 28.235472)" href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=116.11061&amp;materialsCitation.latitude=28.235472">In</a>
                 the Ganfu Channel, Jiangxi Province, China. (August 2019, site one 28°11’8.2” N, 116°10’2.56” E and October 2019,  site two 28°14’7.70” N, 116°6’38.21” E) . 
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            <p>  Holotype here designated: —Dry material of strain CHAB 4136 was stored at the Freshwater Algae Biology Herbarium (HBI),  Institute of Hydrobiology , Chinese Academy of Science, Wuhan, Hubei Province, China, as specimen No. JXGF201901. </p>
            <p>Reference strain: — CHAB 4136 and CHAB 4163 (the additional strain) were deposited at the CHAB, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, China.</p>
            <p>Habitat: —Rock surface in freshwater rivers and channels in subtropical region.</p>
            <p> Molecular and phylogeny analyses: —The 16S rRNA gene sequences of  Microseira minor strains CHAB 4136 and CHAB 4163 obtained in this study shared 98.90%–99.92% similarities among all six clones by calculating p-distance with pairwise deletion of gaps using MEGA software v7.0. These clones shared 95.00%–96.01% with other  Microseira species , less than 97% which was regarded as the cutoff for bacterial species delimitation. These clones had a maximum similarity of 16S rRNA gene sequences as 95.50% with their closest filamentous genera (sharing similarities to Potamosiphon,  Aerosakkonema ,  Phormidium, Potamolinea and  Oscillatoria as 93.98%–95.50%, 92.78%–92.95%, 92.28%–92.44%, 92.11%–92.44% and 91.92%–92.60%, respectively) (Table 1). The 16S rRNA gene phylogenetic trees based on ML and BI methods showed that the six clones of  Microseira minor clustered a unique clade (cluster A) together with other  Microseira species (Fig. 3 and Fig. 4), supported by ML and BI calculations with high bootstrap values of 100% and 1.000. </p>
            <p> In the nifH phylogeny (Fig. 5 and Fig. 6), the eight clones obtained from the two strains of  Microseira minor in this study formed a well-supported freshwater clade together with all environmental clones of  Microseira wollei (cluster M, cluster N and cluster L). The nifH gene sequences of CHAB 4136 (cluster S1) and CHAB 4163 (cluster S2) divided into two distinct sub-groups with cluster M and cluster N, and sequence similarities were 94.28%–95.96% and 96.97%–98.32%, respectively (Table 2). The whole clade formed by  Microseira clones including the two strains of  Microseira minor were supported by ML and BI methods with high bootstrap values of 85% and 0.90, supporting the two strains as a new species  Microseira minor within the genus  Microseira . </p>
            <p> Genes associated with four types of toxins of microcystins, cylindrospermopsin, paralytic shellfish toxins and anatoxin were not detected in any of these  Microseira minor strains. </p>
            <p> Analyses of ITS between 16S and 23S rRNA gene: —The ITS sequences of the six clones from the two strains of  Microseira minor , shared 96.05-96.26% similarities with each other, and contained both tRNA Ile and tRNA Ala, ranging from 485 to 499 bp. </p>
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	https://treatment.plazi.org/id/03F1879F521C8D03FF040B9DFE53312B	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Geng, Ruozhen;Wen, Qianzhi;Wang, Yilang;Yang, Ping;Dai, Guofei;Li, Renhui;Yu, Gongliang	Geng, Ruozhen, Wen, Qianzhi, Wang, Yilang, Yang, Ping, Dai, Guofei, Li, Renhui, Yu, Gongliang (2021): A novel filamentous cyanobacterium Microseira minor sp. nov. (Oscillatoriaceae, Cyanobacteria) from the Ganfu Channel, Jiangxi, China. Phytotaxa 524 (3): 178-190, DOI: 10.11646/phytotaxa.524.3.2
