taxonID	type	description	language	source
03DE879AFFEAFF8DFE8C6788CC33FDD7.taxon	description	Figures 1 – 8	en	Bolton, Samuel J., Klompen, Hans, Bauchan, Gary R., Ochoa, Ronald (2014): A new genus and species of Nematalycidae (Acari: Endeostigmata). Journal of Natural History (J. Nat. Hist.) 48 (23 - 24): 1359-1373, DOI: 10.1080/00222933.2013.859318, URL: http://dx.doi.org/10.1080/00222933.2013.859318
03DE879AFFEAFF8DFE8C6788CC33FDD7.taxon	diagnosis	Diagnosis Osperalycus is readily distinguished from other genera of Nematalycidae by the presence of simple setae of similar size along the opisthosoma (Figure 1 A, B); the simple setae next to the anal opening of Gordialycus and Nematalycus are at least three times as long as those along the rest of the opisthosoma, although the opisthosoma of Gordialycus and Nematalycus is often almost completely nude. Cunliffea and Psammolycus have bifurcate or trifurcate setae along the opisthosoma. Osperalycus is also readily distinguished from Gordialycus and Nematalycus by the baculiform solenidion on tarsus II – this solenidion has a distinctly swollen or bulbous tip in Gordialycus and Nematalycus. The most distinct characters of Osperalycus are to be found in the mouthparts (Figure 2 A, B). The vessel (Ve) – modified from the lateral lips – is a very unusual structure that appears to be unique to this genus (Figure 2 A). Markedly short chelicerae (Ch) (<15 µm) are found in both Osperalycus tenerphagus and Psammolycus delamarei, but not Nearctic cf. Psammolycus or any other genus of Nematalycidae. Osperalycus is also distinct from all other genera in having rutella (Ru) that overlap at the midline (Figure 2 A). Female General morphology. Idiosoma ≈ 600 µm long (≈ 12 times longer than wide) when fully extended (Figure 1 A, B). Cuticle with flat round projections, or palettes, sensu Haupt and Coineau (1999), lining the latitudinal annuli (Figure 3 A – D). Trachea and peritremes absent. Lyrifissures absent from idiosoma. Podocephalic canals terminating anteriorly between the bases of the chelicerae; extending back beyond coxae I. Distinct glands leading into podocephalic canals at coxae I. Long narrow oesophagus extends to proximity of metapodosoma. Prodorsum. Trichobothria absent; naso absent; eyes absent. Three pairs of setae (exa, in and le) and one unpaired rostral (ro) seta. All setae simple and close to the midline of the prodorsum (Figure 2 A). Setae ro, le and in subequal and short (4 – 8); exa long (16 – 22). Opisthosoma. All setae simple. Setae associated with all nine opisthonotal segments: c 1 – 4, d 1 – 2, e 1 – 2, f 1 – 3, h 1 – 2, ps 1 – 3, ad 1 – 3, an 1 – 3, pa 1 – 3. Frequent and noticeable asymmetry in the longitudinal positions of pairs of setae (Figure 1 A, B). In segmental remnants F-PA, ancestrally dorsolateral setae are displaced to a ventrolateral or ventral position that is usually noticeably more anterior than the dorsomedial setae of the same segment. Segment C very long; position of c 2 and c 3 much more posterior than c 1 and c 4. Setae on segments C – E generally shorter (5 – 15) than those on segments F-PA (10 – 19). Setae c 2 short (5 – 8); proximal setae c 3 comparatively long (9 – 13). Longest opisthosomal setae h 2 and ps 3 (15 – 19). From segments H to PA, ventral setae (h 2, ps 3, ad 3, an 3, pa 3) typically 1 to 4 µm longer than lateral and dorsal setae on the same segment; lateral and dorsal setae subequal. Distinct anal valves projecting from the anus (Figure 4 A – C). Ventral furrow, in which annular ridges terminate (Figure 4 D), extending from proximity of anal valves to genital region (Figure 4 A). Three pairs of genital (g) setae and two pairs of aggenital (ag) setae (Figure 1 C). Genital and aggenital setae short (3 – 7) compared with most other opisthosomal setae. Two pairs of bilobed genital papillae (Figure 1 D). Podosoma. Large gap separating legs II and III (Figure 1 B). Coxal fields I and II medially separated by intercoxal region (Figure 2 B). Single pair of intercoxal setae (between coxal fields II). Coxal fields III and IV medially fused (Figure 1 C). Each coxal field I with one bifurcate seta and one simple seta; each coxal field II with one bifurcate seta; coxal fields III with one bifurcate seta and two simple setae on each side of the fused coxal plate; coxal fields IV with one simple seta on each side of the fused coxal plate. Legs. Pretarsi I – IV each with two lateral claws and a ciliated empodium (Figure 5 A – E). Solenidial formulae femora – tarsi for individual legs I – IV: 0 - 1 - 2 - 1; 0 - 0 - 0 - 1; 0 - 0 - 0 - 0; 0 - 0 - 0 - 0. Longitudinal grooves of shortest solenidion phi (φ) (tibia I) restricted to bulbous tip (Figure 5 F). Other solenidia baculiform with longitudinal grooves extending almost all the way to the base. Solenidia omega (ω) on tarsi I and II subequal and noticeably larger than solenidion phi (φ) and sigma (σ) on, respectively, tibia I and genu I (Figure 6). Setae absent from trochanters I – IV. Setal formulae femora – tarsi for individual legs I – IV (excluding solenidia and famuli): 4 - 6 - 7 - 16; 2 - 2 - 2 - 9; 1 - 0 - 2 - 6; 1 - 0 - 2 - 5. All setae on femora – tibiae I – IV simple. Tarsi I – IV with various setae (see below). Femur I sometimes lacking the dorsal seta normally added in post-protonymphal instars – some individuals with three setae on one femur I and four on the other. Tarsus I (Figure 5 A). Seven pairs of setae (p, tc, ft, u, a, pv and pl) and two unpaired setae (s and d). Proral (p) setae conical and extending from turbercle. One tectal (tc ″) and one fastigial (ft ″) seta usually semi-bifurcate, with one branch short and barb-like – sometimes short and basal enough for the seta to qualify as simple (see aforementioned criteria in Material and methods section), or rarely long enough to be bifurcate. Other fastigial (ft') seta usually bifurcate, rarely semi-trifurcate or trifurcate. All other setae simple. Subunguinal (s) seta centred between pair of anterolateral (a) setae. Dorsal (d) seta proximal and posterior to base of solenidion ω. Small stubby famulus (ε) near tc ″. Dorsolateral lyrifissure next to posterior margin of tarsus (obscured by overlying integument when viewed under LT-SEM). Tarsus II (Figure 5 B, C). Four pairs of setae (p, tc, ft and u) and one unpaired seta (d). Setae p, tc and u bifurcate and projecting forward past base of ambulacrum; ft simple; d trifurcate. Long rod-like famulus near tc ″. Base of solenidion ω next to posterior margin of tarsus. Tarsus III (Figure 5 D). Two pairs of setae (p and u) and two unpaired setae (tc ″ and d). Setae p, u and tc ″ bifurcate and projecting forward past base of ambulacrum; d bifurcate. Tarsus IV (Figure 5 E). Identical to tarsus III but with tc ″ absent. Gnathosoma. Gnathosoma including a vessel (Ve) – modified from the lateral lips – into which the chelae (opposing digits) slot (Figure 2 A). Chelicerae (Ch) ≈ 12 µm long and slanting downwards, anteriorly, into the vessel. Single dorsal seta on each chelicera. Rutella (Ru) consisting of a swollen base with a narrow extending digit. The rutella lie against the anterior surface of the vessel and overlap at the midline, appearing as an inverted v – more discernible, under a light microscope, when the chelae are not within the vessel. Palps (Pa) ≈ 10 µm long and three-segmented, tentatively designated femur, genu and tarsus; solenidia absent; setal formula femur – tarsus for each palp: 0 - 1 - 6; distal tarsal seta with cupshaped tip; all other setae simple. Palp tarsus also with three very small cuticular protuberances (only visible under LT-SEM and omitted from Figure 2 A, B). Subcapitulum with four pairs of setae: m, a, or 1 – 2 (Figure 2 B). Adoral setae (or 1 – 2) typically unevenly tapering (or 2 sometimes evenly tapering); anterior pair (or 1) vestigial and broad. Ontogeny Idiosoma and appendages. From larva to adult, the idiosoma increases greatly in length compared with the appendages (Table 1; Figures 7, 8 A, B). Notably, there is no apparent increase in the length of the palp. Chaetotaxy. Aside from the legs and coxal fields (see below), setal additions only occur in the genital region and segments AD to PA (Table 2). Adults readily distinguished from tritonymphs by the presence of setae ag 2 (very posterior and close to f 3) and long pa 1 – 3 (Table 2). New segmental additions of setae to the posterior tip of the opisthosoma always short (3 – 7), increasing two- to four-fold in length at the next instar (Table 2). Several other opisthosomal setae noticeably increase in length from the larval to adult instar (Figures 1 A, B, 7): seta f 3 increases approximately four-fold (from 3 – 4) to be subequal to f 2 and f 1 (11 – 16); setae d 2 and e 2 increase by about 50 % (from 7 – 10 to 10 – 15). All other opisthosomal and prodorsal setae increase only slightly (<30 %) or not at all. Leg setation complete (including coxal fields) by the deutonymphal instar (Table 3). Setation of tarsus I is completed by the addition of an unpaired dorsal (d) seta in the protonymph. Chaetotaxy unchanging for tarsi II – IV. Numbers of solenidia, famuli and lyrifissures also unchanging for all leg segments. Gnathosoma without additions of setae or any other structures.	en	Bolton, Samuel J., Klompen, Hans, Bauchan, Gary R., Ochoa, Ronald (2014): A new genus and species of Nematalycidae (Acari: Endeostigmata). Journal of Natural History (J. Nat. Hist.) 48 (23 - 24): 1359-1373, DOI: 10.1080/00222933.2013.859318, URL: http://dx.doi.org/10.1080/00222933.2013.859318
03DE879AFFEAFF8DFE8C6788CC33FDD7.taxon	materials_examined	Material examined Holotype female (OSAL 015134), USA, Ohio, Franklin Co., Kinnear Road, 39.9990 N, 83.0468 W, silty clay loam from suburban prairie (including shrubs, grasses and small trees); collector: Samuel Bolton, May 2011, 40 cm deep (SB 11 - 05 - I). Same data: 9 F (OSAL 0103239, 0105137, 0105138, 0105147, 0105148, 0105149, 0105153, 0105155, 0105156), 1 TN / F? (OSAL 0105135), 5 TN (OSAL 0105143, 0105146, 0105151, 0105152, 0105157), 1 DN (OSAL 0105145), 1 PN (OSAL 0105150), 3 L (OSAL 0103237, 0105141, 0105142), 1 pharate (TN – F, OSAL 0105154). Same locality and collector, July 2010, 60 cm deep (SB 10 - 0724 - I): 4 F (OSAL 0103240, 0105136, 0105139, 0105140), 1 DN, (OSAL 0105144), 2 PN (OSAL 0103241, 0103242), 1 L (OSAL 0103245), 2 pharates (L-PN, OSAL 0103243, 0103244). August 2011 (LT-SEM material), 60 cm deep: 9 F, 1 TN / F?, 6 TN, 1 DN / TN?, 1 DN, 1 PN (used for SEM, not recovered). USA, Ohio, Ashtabula Co., West Main Road, 41.9246 N, 80.6138 W, sandy loam in small chestnut plantation, collector: Samuel Bolton, July 2011, 40 cm deep (SB 11 - 07 - IV): 1 F (OSAL 0105133), 1 TN (OSAL 0103238), 1 DN (OSAL 0105132). Type material and depositor Holotype female (OSAL 0105134) at Ohio State University Acarology Collection (OSAL), Columbus, Ohio, USA. Paratypes: US National Collection (USNM), housed at the Beltsville Agricultural Research Center, USDA, Beltsville, MD, USA: 2 F (OSAL 0105137, 0105147); Natural History Museum (BMNH), London, UK: 2 F (OSAL 0105140, 0105148); all other paratypes at OSAL.	en	Bolton, Samuel J., Klompen, Hans, Bauchan, Gary R., Ochoa, Ronald (2014): A new genus and species of Nematalycidae (Acari: Endeostigmata). Journal of Natural History (J. Nat. Hist.) 48 (23 - 24): 1359-1373, DOI: 10.1080/00222933.2013.859318, URL: http://dx.doi.org/10.1080/00222933.2013.859318
03DE879AFFEAFF8DFE8C6788CC33FDD7.taxon	etymology	Etymology Binomial. Osperalycus tenerphagus. Ospera - is a combination of the Latin terms for “ mouth ” (os) and “ purse / bag ” (pera) in reference to the soft and unsclerotized vessel of the gnathosoma; - lycus is a Latinized Greek ending given to three of the four other genera of Nematalycidae. The species name, tenerphagus, combines the Latin term for “ tender ” with the Greek-derived Latin suffix for “ feeding ”, referring to the delicate mechanism hypothesized to explain how this mite may carefully pick up small micro-organisms and place them into its feeding vessel without rupturing them (Bolton et al. in preparation). Systematic relationships Whereas rutella were thought to be absent from the Nematalycidae (Walter 2009), they are clearly present in Osperalycus tenerphagus. Rutella also appear to be present in Gordialycus sp. nov. and cf. Psammolycus sp. nov. (pers. obs.). The presence of rutella combined with the absence of a tracheal system more firmly places the Nematalycidae within the Endeostigmata. Other newly observed characters also suggest a closer relationship with the Endeostigmata than the Tydeoidea, e. g. presence of setae for all nine opisthosomal segments and more than two pairs of setae on the C and F segments. Key to the genera of the Nematalycidae	en	Bolton, Samuel J., Klompen, Hans, Bauchan, Gary R., Ochoa, Ronald (2014): A new genus and species of Nematalycidae (Acari: Endeostigmata). Journal of Natural History (J. Nat. Hist.) 48 (23 - 24): 1359-1373, DOI: 10.1080/00222933.2013.859318, URL: http://dx.doi.org/10.1080/00222933.2013.859318
