taxonID	type	description	language	source
03FD141FFF8FFFD4FCAF2809FC21F9E1.taxon	description	http: // lsid. speciesfile. org / urn: lsid: Plecoptera. speciesfile. org: TaxonName: 501137 (Figs. 1 – 20)	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF8FFFD4FCAF2809FC21F9E1.taxon	materials_examined	Material examined. Holotype ♂ U. S. A. – Virginia: Grayson Co., Helton Creek, Rte 783 at gated trail by pond, N 36.62816, W 81.56684, 13 May 2017, C. Verdone, B. C. Kondratieff (INHS, Insect Collection 659585). Paratypes: North Carolina: Avery Co., Shantee Creek, Adams Apple Dr., SW of Foscoe, N 36.12439, W 81.82068, 13 May 2010, B. C. Kondratieff, R. E. Zuellig, D. R. Lenat, R. F. Kirchner, ♂ (CSUIC). Macon Co., Tellico Creek, Tellico Rd. ~ 0.5 mi. W of Sugar Cove Rd., N 35.27688, W 83.54347, 15 May 2017, C. Verdone, B. C. Kondratieff, ♂ (CSUIC). Swain Co., Beech Flats Prong, GSMNP, Hwy 441, N 35.60227, W 83.41534, 16 May 2017, C. Verdone, B. C. Kondratieff, ♂ (CSUIC). Watauga Co., Boone Fork, CR 1561, W of Boone Fork, [N 36.12310, W 81.77006], 2 May 2005, B. C. Kondratieff, R. E. Zuellig, D. R. Lenat, R. F. Kirchner, ♂, 2 ♀ (CSUIC). Tennessee: Sevier Co., trib. to Le Conte Creek, GSMNP, Twin Creeks Uplands Research Lab, N 35.68706, W 83.50096, 16 May 2017, C. Verdone, B. C. Kondratieff, 2 ♂, 2 ♀ (CSUIC). Virginia, Franklin Co., Grassy Fork, [Co. Rd. 619, 2 mi. SE Snow Creek Rd.], [N 36.81097, W 79.74516], 21 May 2012, B. C. Kondratieff, R. F. Kirchner, ♂ (CSUIC). Grayson Co., Same location as holotype, 12 May 2017, C. Verdone, B. C. Kondratieff, J. Richards, R. Agbalog, 4 ♂, 17 ♀ (USNM); Same location as holotype, 13 May 2017, C. Verdone, B. C. Kondratieff, 6 ♂, 71 ♀ (CSUIC); Same location as holotype, 17 May 2017, C. Verdone, B. C. Kondratieff, ♀ (INHS, Insect Collection 659584).	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF8FFFD4FCAF2809FC21F9E1.taxon	distribution	Distribution. U. S. A. – NC, TN, VA (Fig. 22).	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF8FFFD4FCAF2809FC21F9E1.taxon	etymology	Etymology. The new species is named in honor of Dr. R. Edward DeWalt, Illinois Natural History Survey, Champaign, Illinois, for his dedication and numerous contributions to the study of stoneflies. The proposed common name is the “ DeWalt Stripetail ”.	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF8FFFD4FCAF2809FC21F9E1.taxon	description	Male. Macropterous; forewing length 10.6 – 11.3 mm (n = 5). Body length 9.6 – 10.0 mm (n = 5). Head and pronotum pale yellow with contrasting pigmentation. General body color pale yellow with light brown to brown markings. (Fig. 1). Dorsal background of head pale yellow with medium brown bands connecting ocelli; bands widest anteriorly; bands truncate at anterior ocellus; triangular dusky spot anterior to anterior ocellus (Fig. 2). Antennal scape pale yellow with a diffuse diagonal brown stripe; flagellum segments 1 – 7 pale; remaining segments medium brown (Figs. 1 – 2). Pronotum with a wide medial pale stripe; rugosities irregular, raised, medium brown; anterolateral margins medium brown; posterolateral margins pale (Fig. 2). Femora light brown dorsally, covered with uniformly spaced, short, brown setae; femora pale ventrally; tibia light brown, covered with uniformly spaced, short brown setae; tarsi medium brown (Fig. 1). Meso- and metanota light brown (Fig. 1). Wings hyaline, veins of forewing light basally, brown distally (Fig. 1). Terga light brown, posterior ⅓ medium brown; terga 1 – 8 with uniformly spaced brown setae. Sterna pale yellow to light brown; sternum 8 with a light brown recessed vesicle, 0.65 X as wide as long; evenly rounded, extending to the posterior margin of sternum 8 (Fig. 3). Paraprocts short, triangular, slightly incurved, moderately sclerotized; trichoid sensilla scattered over dorsal surface, absent on apex; apex heavily sclerotized; tips acutely pointed (Fig. 4). Cerci brown, setose; cercal segments with a single, long, stout, ventral hair at posterior margin. Aedeagus with a long basal stalk, a highly reduced (vestigial) posteroventral membranous aedeagal tube, a posteroventral lobe, paired posterolateral lobes, a dorsal lobe, and an anterior lobe (Figs. 5 – 10); posteroventral membranous aedeagal tube highly reduced (vestigial), appearing only occasionally as a small lobe at the base of the basal stalk (Fig. 5); aedeagus with a posteromedial rectangular patch of dense, stout, rust colored spines, and a single posterior sclerotized arrowhead shaped aedeagal spine plate with upturned distal stout spine (Figs. 8 – 10); apex of structure upturned with approximately 10 large stout rust colored spines (Fig. 11); base of plate with broad flat sclerotized plates with numerous small apical finger-like projections (Fig. 11); basal stalk with short sharp spinulae (Fig. 12); posterolateral lobes with sparse scale like plates medially; posteromedial area with shallow shelf-like scales with apical hair-like projections (Fig. 13); lateral area with dense short sharp spinulae (Fig. 14). Female. Macropterous; forewing wing length 11.3 – 12.6 mm (n = 5). Body length 9.1 – 10.9 mm (n = 5). Body coloration and morphology similar to male. Sternum 8 with variable subgenital plate extending approximately ⅓ – ½ over sternum 9; subgenital plate broadly rounded (Fig. 15), or broadly triangular, truncate with shallow posteromedian emargination (Fig. 16), or broadly triangular (Fig. 17). Ovum. Length 226.3 – 234.4 μm (n = 2); width 184.9 – 192.5 μm (n = 2). General shape oblong; cross section triangular, distinct cross shaped ridges dividing anterior and posterior poles; anterior end concave (Fig. 18). Color pale brown. Collar well-developed, flared apically with elevated longitudinal ridges; base not offset from egg body (Fig. 19). Hexagonal follicle cell impressions well-developed with thickened ridges and with numerous small mounds between reticulations (Figs. 18, 20). Eclosion line absent, micropyles arranged singularly or in threes, positioned on top of cross ridges near anterior ⅓ of egg (Figs. 18, 20). Larva. Unknown.	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF8FFFD4FCAF2809FC21F9E1.taxon	diagnosis	Diagnosis. Isoperla dewalti is placed in the I. montana Group based on the presence of a single posterior sclerotized arrowhead shaped aedeagal spine plate with an upturned distal stout spine, dark brown bars connecting the ocelli, and – albeit vestigial – a posteroventral membranous aedeagal tube. The new species is easily distinguished from I. montana and I. nelsoni by the lack of a well-developed posteroventral membranous aedeagal tube. Several paratype specimens of I. dewalti that are deposited in the C. P. Gillette Museum of Arthropod Diversity had previously been identified as I. montana. The two species are superficially similar and it was likely thought that the posteroventral membranous aedeagal tube did not evert. However, it is the experience of the authors that when a welldeveloped aedeagal tube is present it is the first structure to evert. Additionally, differences in the posteromedial patch of spines distinguish the two species. In I. dewalti the posteromedial patch of spines is broad, appearing as a uniform rectangular patch (Figs. 8 – 10). Whereas, in I. montana the posteromedial patch of spines is narrow, appearing as a thin ridge (figs. 33.15 – 33.16, Szczytko & Kondratieff 2015). Furthermore, I. montana possesses a dorsal rounded mound of dense, long, stout, rust colored spines above the posteromedial patch of spines (figs. 33.15 – 33.16, Szczytko & Kondratieff 2015), which the new species lacks. Females of the new species are most similar to I. montana and I. kirchneri Szczytko & Kondratieff, 2015. Females of the new species have a subgenital plate that extends approximately ⅓ – ½ over sternum 9 and appears broadly rounded (Fig. 15), or broadly triangular, truncate with shallow posteromedian emargination (Fig. 16), or broadly triangular (Fig. 17), and have a dorsal head pattern with brown bands connecting the ocelli that are truncate at the anterior ocellus with a triangular dusky spot anterior to the anterior ocellus (Fig. 2). Females of I. montana and I. kirchneri also have a subgenital plate that is variable, appearing broadly triangular usually with a shallow posteromedian emargination or pointed (figs. 25.5, 33.7, Szczytko & Kondratieff 2015) and have dorsal head patterns with brown bands connecting the ocelli. In I. montana the bands extend anteriorly to near the frons (occasionally pale brown or faint), and without a medium brown anterior patch near the frons (fig. 33.1, Szczytko & Kondratieff 2015). In I. kirchneri the bands sometimes extend anteriorly from the anterior ocellus, with a well-defined to diffuse spot on frons (fig. 25.1, Szczytko & Kondratieff 2015). The variation in subgenital plate morphology and dorsal head pattern exhibited by these species make the separation of isolated females very difficult. Ova of the new species are similar to I. kirchneri, I. montana, I. nelsoni, and I. signata (Banks, 1902), all of which are oblong and triangular in cross section with distinct cross shaped ridges dividing anterior and posterior poles. Ova of the new species are distinguished from other similar ova by the interreticulate follicle cell impressions, which are developed as mounds in the new species (Figs. 18, 20) and punctations in the other similar species (figs. 25.19 – 25.22, 33.25 – 33.28, 36.9 – 36.11, 50.20 – 50.23, Szczytko & Kondratieff 2015).	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF8FFFD4FCAF2809FC21F9E1.taxon	biology_ecology	Biological notes. There is no information about the biology or life cycle of I. dewalti. Based on the above records, the emergence period appears to be in early to mid-May, and as with most eastern Isoperla, a univoltine life cycle is presumed. The type locality, Helton Creek, has a unique stonefly fauna, species with generally northern distributions (e. g. Alloperla concolor Ricker, 1936) and species with generally southern distributions (e. g. Haploperla parkeri Kirchner & Kondratieff, 2005) have been documented from this location. From the limited records available it is inferred that the new species also has a generally southern distribution, occurring in 1 st – 3 rd order streams in the Piedmont Plateau and the southern Blue Ridge physiographic provinces (Fig. 22).	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF84FFD0FC452F6EFB4DFE04.taxon	description	http: // lsid. speciesfile. org / urn: lsid: Plecoptera. speciesfile. org: TaxonName: 468740	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF84FFD0FC452F6EFB4DFE04.taxon	vernacular_names	Montane Stripetail	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF84FFD0FC452F6EFB4DFE04.taxon	materials_examined	Holotype ♂ (MCWC), Mount W ashington, Coos Co., New Hampshire.	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF84FFD0FC452F6EFB4DFE04.taxon	materials_examined	Material examined. U. S. A. – New York: Orange Co., spring trib. to Ramapo River, Rt. 17 N Arden Rd., N 41.27410, W 74.15320, 21 May 2008, L. Myers, B. C. Kondratieff, R. W. Baumann, 23 ♂, 3 ♀, 3 N (CSUIC). Oswego Co., Little Sandy Creek, Hwy 13, S Locana, [N 43.64889, W 76.006814], 17 June 1987, B. C. Kondratieff, R. W. Baumann, ♂, ♀ (CSUIC); Salmon River, CR 17, upstream of reservoir, N 43.58740, W 75.84780, 28 May 2008, L. Myers, B. C. Kondratieff, 2 ♂, 2 ♀ (CSUIC). Rensselaer Co., Dill Brook, Dill Brook Rd., W Stillham, N 42.76300, W 73.35490, 19 April 2009 (emerged 28 April 2009), L. Myers, B. C. Kondratieff, 2 ♂, ♀ (CSUIC). Saratoga Co., Alplaus Kill, Rt. 67, N 42.97420, W 74.01970, 26 May 2009, L. Myers, ♂ (CSUIC). Ulster Co., Lucas Kill, Ralph Vedder Rd., [N 42.140024, W 74.022707], 21 May 2008, B. C. Kondratieff, 4 ♂, 3 ♀ (CSUIC). Virginia: Montgomery Co., Mill Creek, Rt. 785, [N 37.26133, W 80.34061], 12 April 1979, R. Wallace, ♂ (CSUIC); North Fork Roanoke River, Rt. 603, [N 37.18667, W 80.352603], 18 May 1980, M. Spencer, ♂, 2 ♀ (CSUIC). Russell Co., Loop Creek, Rte 619, [N 36.890811, W 81.953779], 12 April 1980, B. C. Kondratieff, ♂ (CSUIC). Smyth Co., North Fork Holston River, Rt. 42, Saltville, [N 36.89084, W 81.749537], 20 May 1993, B. C. Kondratieff, R. F. Kirchner, ♀ (CSUIC). Tazewell Co., headwaters Station Spring, MBC Ranch off Rt. 666, Burkes Garden, [N 37.094939, W 81.38785], 8 May 1982, B. C. Kondratieff, 4 ♂, 3 ♀ (CSUIC); Station Spring Creek, MBC Ranch, [N 37.100225, W 81.370093], 28 May 1992, B. C. Kondratieff, 5 ♂, 8 ♀ (CSUIC); Same location, 17 May 1994, B. C. Kondratieff, R. F. Kirchner, 8 ♂, 7 ♀ (CSUIC); Station Spring Creek, Rt. 623, Moore Bros. Farm, [N 37.104181, W 81.346915], 18 May 1979, B. C. Kondratieff, 10 ♂, 18 ♀ (CSUIC). West Virginia: Webster Co., Back Fork Elk River, Webster Spring, [N 38.480435, W 80.413394], 9 May 1980, J. Hill, ♂, ♀ (CSUIC). New Records. Canada – New Brunswick: Albert Co., Kennebecasis River, Rte 895, [N 45.826391, W 65.205347], 3 June 1998, B. C. Kondratieff, R. W. Baumann, 3 ♀ (CSUIC). Northumberland Co., Big Eskedelloc River, Hwy 8, [N 47.28819, W 65.405769], 17 June 1999, B. C. Kondratieff, R. W. Baumann, ♂ (CSUIC). Nova Scotia: Antigonish Co., Tracadie River, Hwy 16, S Monastery, [N 45.576041, W 61.586629], 22 June 1993, B. C. Kondratieff, R. W. Baumann, ♂ (CSUIC). Colchester Co., Baileys Brook, Rt. 256, W The Falls, [N 45.640165, W 63.250553], 3 June 1998, B. C. Kondratieff, R. W. Baumann, ♂, 6 ♀ (CSUIC). Iverness Co., N of Melford, Glenn Brook, Hwy 105, [N 45.877216, W 61.25576], 11 June 1998, B. C. Kondratieff, R. W. Baumann, ♂ (CSUIC). U. S. A. – New Hampshire: Strafford Co., Mad River, 2 km SW Farmington, [N 43.37762, W 71.093572], 5 June 2011, D. S. Chandler, ♂, ♀ (CSUIC). New York: Clinton Co., spring, Recore Rd., N 44.81860, W 73.54110, 16 March 2009 (emerged 15 April 2009), L. Myers, ♂, ♀ (CSUIC); trib. to True Brook, Clark Hill Rd., N 44.65880, W 73.80220, 1 June 2007, J. Mihuc, ♂ (CSUIC); Same location, 13 – 18 June 2008, J. Mihuc, ♀ (CSUIC); Same location, 30 May – 8 June 2008, J. Mihuc, ♂, ♀ (CSUIC); True Brook, True Brook Rd., [N 44.63703, W 73.769705], 16 May 2006, L. Myers, ♀ (CSUIC). Cortland Co., Gridley Creek, Rt. 392 nr. East Virgil, N 42.4944, W 76.1062, 19 February 2008, L. Myers, ♂, 2 ♀ (CSUIC). Delaware Co., spring-fed trib. to Emory Brook, Rt. 28 Fleischmanns, N 42.15110, W 74.52240, 27 May 2009, L. Myers, B. C. Kondratieff, ♀ (CSUIC). Essex Co., Pettigrew Brook, Bonnieview Rd. nr. Willington, [N 44.43083, W 73.800231], 26 June 2007, L. Myers, B. C. Kondratieff, ♂, 2 ♀ (CSUIC). Greene Co., [Shingle Kill], Maple Lawn Rd., [N 42.265848, W 74.032405], 24 June 2007, L. Myers, B. C. Kondratieff, ♂, ♀ (CSUIC); Kiskatom Brook, Rt. 32 nr. Kiskatom, N 42.22170, W 73.98720, 26 May 2009, L. Myers, B. C. Kondratieff, ♀ (CSUIC). Saratoga Co., small stream, Alpine Meadows Rd., N 43.17860, W 73.90450, 12 February 2009 (emerged 20 April 2009), L. Myers, ♂ (CSUIC). Warren Co., Glen Creek, Glen Creek Rd., N 43.58510, W 73.87130, 15 May 2008, L. Myers, E. White, ♀ (CSUIC). Pennsylvania: Clinton Co., Fishing Creek, Sieg Conference Center, Marrow Rd., [N 40.988602, W 77.497564], 8 June 2013, B. C. Kondratieff, J. Sandberg, 27 ♀ (CSUIC); Fishing Creek, Sieg Conference Center, Marrow Rd., [N 40.988602, W 77.497564], 9 June 2013, B. C. Kondratieff, J. Sandberg, 3 ♂ CSUIC). Crawford Co., trib. to French Creek, Hwy 19, 1 mi. S Cambridge Springs, [N 41.796281, W 80.077902], 17 April 1979, S. W. Szczytko, K. W. Stewart, ♂, 2 ♀ (CSUIC). Mifflin Co., Honey Creek, Reeds Gap State Park, Lancaster Valley Rd., [N 40.766351, W 77.370555], 7 June 2013, B. C. Kondratieff, J. Sandberg, 2 ♀ (CSUIC). Virginia: Bland Co., Kimberling Creek, Hwy 42, Rte 606, N 37.16646, W 80.90034, 7 May 2017, C. Verdone, B. C. Kondratieff, S. Roble, ♂ (CSUIC); Wolf Creek, Grapefield Rd., Stephen Levitt Property, N 37.14702, W 81.26314, 4 June 2016, C. Verdone, B. C. Kondratieff, 3 ♂, 4 ♀ (CSUIC); Same location, 6 June 2016, C. Verdone, B. C. Kondratieff, 5 ♂, 6 ♀ (CSUIC); Same location, 8 June 2016, C. Verdone, B. C. Kondratieff, 3 ♀ (CSUIC); Same location, 23 May 2017, C. Verdone, ♂, 2 ♀ (CSUIC); Same location, 25 May 2017, C. Verdone, D. Fuller, ♂, 2 ♀ (CSUIC); Same location, 27 May 2017, C. Verdone, D. Fuller, ♂ (CSUIC); Wolf Creek, Rte 614, Grapefield Rd., Wolf Creek Picnic Area, N 37.18026, W 81.19496, 7 May 2017, C. Verdone, B. C. Kondratieff, S. Roble, 2 ♀ (CSUIC). Botetourt Co., North Creek, Jefferson National Forest, [N 37.541442, W 79.586821], 27 April 1981, J. Widlack, ♀ (CSUIC). Montgomery Co., Mill Creek, Rt. 785, [N 37.26133, W 80.34061], 4 April 1978 (emerged 15 April 1978), B. C. Kondratieff, 2 ♂, 3 ♀ (CSUIC); Same location, 15 April 1980, B. C. Kondratieff, ♀ (CSUIC); Same location, 26 April 1980, B. C. Kondratieff, 2 ♂, 7 ♀ (CSUIC); North Fork Roanoke River, Rt. 603, [N 37.18667, W 80.352603], 30 April 1978, B. C. Kondratieff, ♀ (CSUIC); small trib. to South Fork Roanoke River, Rte 637, [No GPS], 5 June 1981, A. Beck, 25 ♀ (CSUIC). Rappahannock Co., Rocky Run, Rt. 707, [N 38.577655, W 78.28196], 7 July 1979, Firth, ♀ (CSUIC). Smyth Co., North Fork Holston River, Co. Rd. 620, jct. Co. Rd. 716, [N 36.94807, W 81.49062], 27 May 1992, B. C. Kondratieff, ♂, 2 ♀ (CSUIC); Same location, 20 May 1993, B. C. Kondratieff, ♂, 5 ♀ (CSUIC); Same location, 16 May 2004, B. C. Kondratieff, R. F. Kirchner, R. E. Zuellig, ♂, ♀ (CSUIC). Tazewell Co., Cove Creek, Rte 662, off Hwy 61, 37.17837, W 81.29900, 6 June 2016, C. Verdone, B. C. Kondratieff, ♂ (CSUIC); Same location, 7 May 2017, C. Verdone, B. Kondratieff, S. Roble, 4 ♂, 10 ♀ (CSUIC); spring nr. spring house, Cove Creek, Rte, N 37.17989, W 81.30067, 7 May 2017, C. Verdone, B. Kondratieff, S. Roble, 2 ♀ (CSUIC).	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF84FFD0FC452F6EFB4DFE04.taxon	distribution	Distribution. Canada – NS, ON, PQ. U. S. A. – CT, DE, IN, KY, ME, MN, NC, NH, NJ, NY, OH, PA, VA, WV (Szczytko & Kondratieff 2015).	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF84FFD0FC452F6EFB4DFE04.taxon	biology_ecology	Biological notes. Isoperla montana is the most widespread species of the I. montana Group occurring from New Brunswick, Canada to North Carolina and west to Indiana (Szczytko & Kondratieff 2015). The majority of Virginia records are from the Ridge and Valley Physiographic Province with few records east of this province, particularly in the southern part of the state. Szczytko and Kondratieff (2015) reported I. montana from North Carolina based on two females collected by the authors (CJV, BCK). As noted above, species determination of isolated females is difficult, especially in the southern Appalachians where the ranges of I. montana and I. kirchneri overlap, as such this record should be regarded as tentative. In addition to the material listed above, we also examined specimens of I. montana reported by Grubbs (2016) from Alabama. These specimens appear to represent an undescribed taxon based on details of the aedeagus and ovum, but more specimens with the aedeagus everted and mature ova are needed for confirmation.	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF80FFD3FCAC2B05FB41FE53.taxon	description	http: // lsid. speciesfile. org / urn: lsid: Plecoptera. speciesfile. org: TaxonName: 468741	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF80FFD3FCAC2B05FB41FE53.taxon	vernacular_names	Nelson Stripetail	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF80FFD3FCAC2B05FB41FE53.taxon	materials_examined	Holotype ♂ (USNM), Clear Creek, Morgan Co., Tennessee.	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF80FFD3FCAC2B05FB41FE53.taxon	materials_examined	Holotype ♂ (USNM), 3 mi. NE Mathias, 38 ° 55 ’ N, 78 ° 49 ’ W, Hardy Co., West Virginia. Syn. n. Material examined. U. S. A. – Georgia: Dade Co., [Daniel Creek], Rt. 136, 9.1 mi. W jct. 176 / 193, just past entrance Cloudland State Park, [N 34.816158, W 85.491197], 8 May 1980, C. H. Nelson, 3 ♂, ♀ (CSUIC, paratypes). North Carolina: Macon Co., Bridal Veil Falls, nr. Highlands, [N 35.071766, W 83.229483], 8 May 2000, B. Stark, B. Bryan, ♂ (CSUIC, paratype). Tennessee: Hamilton Co., Shoal Creek, Shoal Creek Rd. off Rt. 127, Signal Mountain, [N 35.117311, W 85.353965], 29 May 1981, C. H. Nelson, 3 ♂, 3 ♀ (CSUIC, paratypes). Sequatchie Co., Brush Creek, N Dunlap, [N 35.404253, W 85.369517], 12 May 2000, B. C. Kondratieff, R. F. Kirchner, ♂ (CSUIC, paratype). Virginia: Montgomery Co., small trib. Craig Creek, 0.6 mi. E Hwy 460, [N 37.29070, W 80.45221, 1 May 1992, B. C. Kondratieff, 4 ♂, 7 ♀ (CSUIC, paratypes, I. smithi); spring-fed stream, Rte 621, ~ 0.5 off State Rte 460, [N 37.29070, W 80.45221], 29 May 1979, B. C. Kondratieff, 5 ♂ (CSUIC). Tazewell Co., springs into Station Spring Creek, Burkes Garden, [N 37.097691, W 81.373222], 18 May 1979, B. C. Kondratieff, 2 ♂ (CSUIC, paratypes); West Virginia: Hardy Co., Lower Cove Run, 3 mi. NE of Mathias, N 38.916667, W 78.816667, 16 – 31 May 2000, D. Smith, 4 ♂, 7 ♀ (CSUIC, paratypes, I. smithi). Kanawha Co., Buzzard Branch, I- 64 / 77, [N 38.11827, W 81.38546], 16 May 1994, B. C. Kondratieff, R. F. Kirchner, 2 ♀ (CSUIC, paratypes, I. smithi). New Records. Kentucky: Rowan Co., Triplett Creek, 0.4 mi. N of I- 64, Conley Rd., (No GPS), 25 May 1994, B. C. Kondratieff, R. F. Kirchner, 1 ♂ (CSUIC). Tennessee: Cumberland Co., 3 mi. N of Adams Bridge Obed River, N 36.10321, W 84.94164, 22 May 1994, B. C. Kondratieff, R. F. Kirchner, 2 ♂ (CSUIC, paratypes). Franklin Co., seeps on Hwy 64, 4 mi. E Cowan, [N 35.167581, W 86.074139], 28 May 1994, B. Stark, ♂, ♀ (CSUIC). Virginia: Bland Co., Wolf Creek, Rte 614, Stephen Levitt Property, N 37.14702, W 81.26314, 25 May 2017, C. Verdone, D. Fuller, ♂, ♀ (CSUIC). Giles Co., spring, Rte 613 NE side of Mountain Lake, N 37.37012, W 80.52918, 28 May 2016, C. Verdone, B. C. Kondratieff, ♂, ♀ (CSUIC). Scott Co., Laurel Branch, FR 700,> 1 km from FR 701, N 36.87556, W 82.48100, 6 May 2017, C. Verdone, B. C. Kondratieff, S. Roble, ♂ (CSUIC); Laurel Branch and trib., FR 700, N 36.87844, W 82.46630, 6 May 2017, C. Verdone, B. C. Kondratieff, S. Roble, 4 ♂ (CSUIC). Tazewell Co., Station Spring Creek, MBC Ranch Rd., Burkes Garden, [N 37.100362, W 81.370206], 17 May 1994, B. C. Kondratieff, R. F. Kirchner, 2 ♂ (CSUIC). West Virginia: Tucker Co., Fernow Experimental Forest, [N 39.054807, W 79.669890], 20 June 1990, emergence trap, DPE, 4 ♂, 4 ♀ (CSUIC); Same location, 16 – 23 May 1990, emergence trap DPE, 2 ♀ (CSUIC); Same location, 20 – 26 July 1990, emergence trap, DPE, 1 ♂ 2 ♀ (CSUIC); Same location, 21 May – 5 June 1990, emergence trap, DPE, 1 ♂ (CSUIC). Distribution. U. S. A. – AL, GA, KY, NC, TN, VA, WV (Szczytko & Kondratieff 2015). Biological notes. The paratype series of I. smithi from the type locality included a male with the aedeagus everted and females with mature ova, which was previously unknown. These specimens were examined and were determined to be conspecific with I. nelsoni based on details of the aedeagus, female subgenital plate and ova, which were examined using SEM (Figs. 23 – 25). With the synonymy of these species the range of I. nelsoni is extended northward to include locations in Hardy, Kanawha, and Tucker counties, West Virginia. Isoperla nelsoni occurs in pristine springs to medium – large streams from the Interior Low Plateau of south central Tennessee, east to the Blue Ridge in North Carolina and north to the Appalachian Plateau of West Virginia. Although uncommonly collected, I. nelsoni may be more common than records indicate given the wide range of habitats this species can occur.	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF80FFD3FCAC2B05FB41FE53.taxon	distribution	Distribution. U. S. A. – AL, GA, KY, NC, TN, VA, WV (Szczytko & Kondratieff 2015).	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
03FD141FFF80FFD3FCAC2B05FB41FE53.taxon	biology_ecology	Biological notes. The paratype series of I. smithi from the type locality included a male with the aedeagus everted and females with mature ova, which was previously unknown. These specimens were examined and were determined to be conspecific with I. nelsoni based on details of the aedeagus, female subgenital plate and ova, which were examined using SEM (Figs. 23 – 25). With the synonymy of these species the range of I. nelsoni is extended northward to include locations in Hardy, Kanawha, and Tucker counties, West Virginia. Isoperla nelsoni occurs in pristine springs to medium – large streams from the Interior Low Plateau of south central Tennessee, east to the Blue Ridge in North Carolina and north to the Appalachian Plateau of West Virginia. Although uncommonly collected, I. nelsoni may be more common than records indicate given the wide range of habitats this species can occur.	en	Verdone, Chris J., Kondratieff, Boris C. (2017): A New Species Of Isoperla Banks (Plecoptera: Perlodidae) From The Southern Appalachians, With Notes On The I. Montana Group. Illiesia 13 (12): 111-126, DOI: http://doi.org/10.5281/zenodo.4757785
